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Investigating the functional architecture of microbial genomes with synthetic approaches

Objective

The folding of eukaryotic and prokaryotic chromosomes consists of an assortment of intertwined structural features. The resulting complex networks of contacts is highly dynamic and interacts functionally with, or regulates metabolic processes ranging from gene expression to chromosome segregation. Some higher order structures involve evolutionary conserved molecular players, such as structural maintenance of chromosomes (SMC) proteins, while others depend on phylum specific proteins. The functional organization of yeast and bacteria chromosomes are actively investigated, with multiple folded structures uncovered in recent years. However, disambiguating the intermingled structures is a difficult task, limiting their functional characterization. In addition, current technologies are limited and are unable to track the genome-wide folding of duplicated sister chromatids (SC) molecules, limiting the study of genome folding during replication and mitosis.

The overall aim of the SynarchiC project is to characterize, through innovative derivatives of the chromosome conformation capture technology combined with synthetic chromosomes, the folding patterns of microbial genomes during the entire cell cycle, including those of SCs. By reverse engineering chromosomes in bacteria and yeast, we will discriminate the different layers of topological structures and their associated molecular players. We will then investigate how these 3D structures affect SC folding, individualization, and segregation. Finally, we will investigate the interplay between a pathogen and its hosts during an infectious process. How the bacteria redirects its host chromosome metabolism in stressful environment will be addressed from the perspective of genome organization and segregation. Technologies and results from SynarchiC will provide fundamental insights on the cell cycle, and should appeal broadly to scientists working on various aspects genome functional organization in any clade.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-COG - Consolidator Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2017-COG

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Host institution

INSTITUT PASTEUR
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 995 557,00
Address
RUE DU DOCTEUR ROUX 25-28
75724 Paris
France

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Region
Ile-de-France Ile-de-France Hauts-de-Seine
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 995 557,00

Beneficiaries (1)

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