Nuclear magnetic resonance spectroscopy of liquid-liquid phase separation

Project Information

LLPS-NMR

Grant agreement ID: 787679

DOI

10.3030/787679

Project closed

EC signature date 17 April 2018

Start date 1 September 2018

End date 31 August 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 460 278,00

EU contribution

€ 2 460 278,00

Coordinated by

DEUTSCHES ZENTRUM FUR NEURODEGENERATIVE ERKRANKUNGEN EV
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (42)

A NAC domain mutation (E83Q) unlocks the pathogenicity of human alpha-synuclein and recapitulates its pathological diversity

Author(s): Senthil T. Kumar; Anne-Laure Mahul-Mellier; Ramanath Narayana Hegde; Gwladys Rivière; Rani Moons; Alain Ibáñez de Opakua; Pedro Magalhães; Iman Rostami; Sonia Donzelli; Frank Sobott; Markus Zweckstetter; Hilal A. Lashuel
Published in: Science Advances, Issue 1, 2022, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/sciadv.abn0044

Driving forces behind phase separation of the carboxy-terminal domain of RNA polymerase II

Author(s): David Flores-Solis, Irina P. Lushpinskaia, Anton A. Polyansky, Arya Changiarath, Marc Boehning, Milana Mirkovic, James Walshe, Lisa M. Pietrek, Patrick Cramer, Lukas S. Stelzl, Bojan Zagrovic, Markus Zweckstetter
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-41633-8

Proteasomal degradation of the intrinsically disordered protein tau at single-residue resolution

Author(s): T. Ukmar-Godec, P. Fang, A. Ibáñez de Opakua, F. Henneberg, A. Godec, K.-T. Pan, M.-S. Cima-Omori, A. Chari, E. Mandelkow, H. Urlaub, M. Zweckstetter
Published in: Science Advances, Issue 6/30, 2020, Page(s) eaba3916, ISSN 2375-2548
Publisher: American Association for the Advancement of Science
DOI: 10.1126/sciadv.aba3916

Solid-phase fluorescent BODIPY–peptide synthesis via in situ dipyrrin construction

Author(s): Yue Wu, Wing-Sze Tam, Ho-Fai Chau, Simranjeet Kaur, Waygen Thor, Wei Shen Aik, Wai-Lun Chan, Markus Zweckstetter, Ka-Leung Wong
Published in: Chemical Science, Issue 11/41, 2020, Page(s) 11266-11273, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc04849f

Contributions of the N‐terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA‐induced phase separation

Author(s): Milan Zachrdla; Adriana Savastano; Alain Ibáñez de Opakua; Maria‐Sol Cima‐Omori; Markus Zweckstetter
Published in: Protein Science, Issue 1, 2022, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4409

Disease‐Associated Tau Phosphorylation Hinders Tubulin Assembly within Tau Condensates

Author(s): Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Published in: Angewandte Chemie International Edition, Issue 60/2, 2021, Page(s) 726-730, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202011157

Impact of distinct FG nucleoporin repeats on Nup98 self-association

Author(s): Alain Ibáñez de Opakua, Christian F. Pantoja, Maria-Sol Cima-Omori, Christian Dienemann, Markus Zweckstetter
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-48194-4

Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding

Author(s): Dajun Sang; Tong Shu; Christian F. Pantoja; Alain Ibáñez de Opakua; Markus Zweckstetter; Liam J. Holt
Published in: Molecular Cell, Issue 1, 2022, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2022.08.016

Global Structure of the Intrinsically Disordered Protein Tau Emerges from its Local Structure

Author(s): Lukas S. Stelzl; Lisa M. Pietrek; A. Holla; J. S. Oroz; M. Sikora; J. Köfinger; Benjamin Schuler; Markus Zweckstetter; Gerhard Hummer
Published in: JACS Au, Issue 1, 2022, ISSN 2691-3704
Publisher: American Chemical Society
DOI: 10.1101/2021.11.23.469691

Phase Separation in Biology and Disease; Current Perspectives and Open Questions

Author(s): Steven Boeynaems, Shasha Chong, Jörg Gsponer, Liam Holt, Dragomir Milovanovic, Diana M Mitrea, Oliver Mueller-Cajar, Bede Portz, John F Reilly, Christopher D Reinkemeier, Benjamin R Sabari, Serena Sanulli, James Shorter, Emily Sontag, Lucia Strader, Jeanne Stachowiak, Stephanie C Weber, Michael White, Huaiying Zhang, Markus Zweckstetter, Shana Elbaum-Garfinkle, Richard Kriwacki
Published in: Journal of Molecular Biology, 2023, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2023.167971

Multivalent Tau/PSD-95 interactions arrest in vitro condensates and clusters mimicking the postsynaptic density

Author(s): Zheng Shen, Daxiao Sun, Adriana Savastano, Sára Joana Varga, Maria-Sol Cima-Omori, Stefan Becker, Alf Honigmann, Markus Zweckstetter
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-42295-2

Dynamic Aha1 co‐chaperone binding to human Hsp90

Author(s): Javier Oroz, Laura J. Blair, Markus Zweckstetter
Published in: Protein Science, Issue 28/9, 2019, Page(s) 1545-1551, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3678

Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex

Author(s): Javier Oroz, Bliss J. Chang, Piotr Wysoczanski, Chung-Tien Lee, Ángel Pérez-Lara, Pijush Chakraborty, Romina V. Hofele, Jeremy D. Baker, Laura J. Blair, Jacek Biernat, Henning Urlaub, Eckhard Mandelkow, Chad A. Dickey, Markus Zweckstetter
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06880-0

Multimodal interactions drive chromatin phase separation and compaction

Author(s): Tina Ukmar-Godec, Maria-Sol Cima-Omori, Zhadyra Yerkesh, Karthik Eswara, Taekyung Yu, Reshma Ramesh, Gwladys Riviere, Alain Ibanez de Opakua, Wolfgang Fischle, Markus Zweckstetter
Published in: Proceedings of the National Academy of Sciences, Issue 120, 2024, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2308858120

Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions

Author(s): Rosa Antón, Miguel Á. Treviño, David Pantoja-Uceda, Sara Félix, María Babu, Eurico J. Cabrita, Markus Zweckstetter, Philip Tinnefeld, Andrés M. Vera, Javier Oroz
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-46236-5

Liquid–liquid phase separation of tau: From molecular biophysics to physiology and disease

Author(s): Sandeep K. Rai, Adriana Savastano, Priyanka Singh, Samrat Mukhopadhyay, Markus Zweckstetter
Published in: Protein Science, 2021, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4093

Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils

Author(s): Pijush Chakraborty, Gwladys Rivière, Shu Liu, Alain Ibáñez de Opakua, Rıza Dervişoğlu, Alina Hebestreit, Loren B. Andreas, Ina M. Vorberg, Markus Zweckstetter
Published in: Nature Communications, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-24362-8

Lysine/RNA-interactions drive and regulate biomolecular condensation

Author(s): Tina Ukmar-Godec, Saskia Hutten, Matthew P. Grieshop, Nasrollah Rezaei-Ghaleh, Maria-Sol Cima-Omori, Jacek Biernat, Eckhard Mandelkow, Johannes Söding, Dorothee Dormann, Markus Zweckstetter
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-10792-y

Aromaticity at position 39 in α‐synuclein: A modulator of amyloid fibril assembly and membrane‐bound conformations

Author(s): Fiamma A. Buratti; Nicola Boeffinger; Hugo A. Garro; Jesica S. Flores; Francisco J. Hita; Phelippe do Carmo Gonçalves; Federico dos Reis Copello; Leonardo Lizarraga; Giulia Rossetti; Paolo Carloni; Markus Zweckstetter; Tiago F. Outeiro; Stefan Eimer; Christian Griesinger; Claudio O. Fernández
Published in: Protein science 31(7), e4360 (2022). doi:10.1002/pro.4360, Issue 1, 2022, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4360

Potent Tau aggregation inhibitor D-peptides selected against Tau-repeat 2 using mirror image phage display

Author(s): Marwa Nidal Malhis; Senthilvelrajan Kaniyappan; Isabelle Aillaud; Ram Reddy Chandupatla; Lisa Marie Ramirez; Markus Zweckstetter; Anselm H. C. Horn; Anselm H. C. Horn; Eckhard Mandelkow; Heinrich Sticht; Susanne Aileen Funke
Published in: ChemBioChem, Issue 1, 2021, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100287

NMR hawk-eyed view of AlphaFold2 structures.

Author(s): Markus Zweckstetter; Markus Zweckstetter
Published in: Protein Science : A Publication of the Protein Society, Issue 1, 2021, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4175

Peptidyl Prolyl Isomerase A Modulates the Liquid–Liquid Phase Separation of Proline-Rich IDPs

Author(s): Maria Babu; Filippo Favretto; Marija Rankovic; Markus Zweckstetter
Published in: Journal of the American Chemical Society, Issue 1, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c07149

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

Author(s): Adriana Savastano, Alain Ibáñez de Opakua, Marija Rankovic, Markus Zweckstetter
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-19843-1

Methylene blue accelerates liquid-to-gel transition of tau condensates impacting tau function and pathology

Author(s): Yongqi Huang, Jitao Wen, Lisa-Marie Ramirez, Eymen Gümüşdil, Pravin Pokhrel, Viet H. Man, Haiqiong Ye, Yue Han, Yunfei Liu, Ping Li, Zhengding Su, Junmei Wang, Hanbin Mao, Markus Zweckstetter, Sarah Perrett, Si Wu, Meng Gao
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-41241-6

Interplay between tau and α-synuclein liquid-liquid phase separation.

Author(s): Anna Siegert; Marija Rankovic; Filippo Favretto; Tina Ukmar-Godec; Timo Strohäker; Stefan Becker; Markus Zweckstetter; Markus Zweckstetter
Published in: Protein Science : A Publication of the Protein Society, Issue 1, 2021, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4025

Solid-state NMR investigation of the involvement of the P2 region in tau amyloid fibrils

Author(s): Savastano, Adriana; Jaipuria, Garima; Andreas, Loren; Mandelkow, Eckhard; Zweckstetter, Markus
Published in: Crossref, Issue 1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-78161-0

The pathogenic R5L mutation disrupts formation of Tau complexes on the microtubule by altering local N-terminal structure

Author(s): Alisa Cario, Adriana Savastano, Neil B. Wood, Zhu Liu,Michael J. Previs, Adam G. Hendricks, Markus Zweckstetter, Christopher L. Berger
Published in: Proceedings of the National Academy of Sciences, 2022, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2114215119

Quaternary structure of patient-homogenate amplified α-synuclein fibrils modulates seeding of endogenous α-synuclein

Author(s): Benedikt Frieg; James A. Geraets; Timo Strohäker; Christian Dienemann; Panagiota Mavroeidi; Byung Chul Jung; Woojin S. Kim; Seung-Jae Lee; Maria Xilouri; Markus Zweckstetter; Gunnar F. Schröder
Published in: Communications biology 5(1), 1040 (2022). doi:10.1038/s42003-022-03948-y, Issue 1, 2022, ISSN 2399-3642
Publisher: Springer
DOI: 10.1038/s42003-022-03948-y

Molecular interactions of FG nucleoporin repeats at high resolution

Author(s): Alain Ibáñez de Opakua, James A Geraets, Benedikt Frieg, Christian Dienemann, Adriana Savastano, Marija Rankovic, Maria-Sol Cima-Omori, Gunnar F Schröder, Markus Zweckstetter
Published in: Nature Chemistry, 2022, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-022-01035-7

Residue-specific identification of phase separation hot spots of Alzheimer's-related protein tau

Author(s): Susmitha Ambadipudi, Jithender G. Reddy, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Published in: Chemical Science, Issue 10/26, 2019, Page(s) 6503-6507, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9sc00531e

Biomolecular condensation of the microtubule-associated protein tau

Author(s): Tina Ukmar-Godec, Susanne Wegmann, Markus Zweckstetter
Published in: Seminars in Cell & Developmental Biology, Issue 99, 2020, Page(s) 202-214, ISSN 1084-9521
Publisher: Academic Press
DOI: 10.1016/j.semcdb.2019.06.007

RNA polymerase II clustering through carboxy-terminal domain phase separation

Author(s): Marc Boehning, Claire Dugast-Darzacq, Marija Rankovic, Anders S. Hansen, Taekyung Yu, Herve Marie-Nelly, David T. McSwiggen, Goran Kokic, Gina M. Dailey, Patrick Cramer, Xavier Darzacq, Markus Zweckstetter
Published in: Nature Structural & Molecular Biology, Issue 25/9, 2018, Page(s) 833-840, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-018-0112-y

Conformational Dynamics of Intrinsically Disordered Proteins Regulate Biomolecular Condensate Chemistry.

Author(s): Anton Abyzov; Martin Blackledge; Markus Zweckstetter
Published in: Crossref, Issue 1, 2022, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.1c00774

Contributions of the N-terminal intrinsically disordered region of the SARS-CoV-2 nucleocapsid protein to RNA-induced phase separation

Author(s): Milan Zachrdla, Adriana Savastano, Alain Ibáñez de Opakua, Maria-Sol Cima-Omori, Markus Zweckstetter
Published in: Protein Science, 2022, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1101/2022.03.30.486418

Reorientational Dynamics of Amyloid-β from NMR Spin Relaxation and Molecular Simulation

Author(s): Nasrollah Rezaei-Ghaleh, Giacomo Parigi, Markus Zweckstetter
Published in: The Journal of Physical Chemistry Letters, Issue 10/12, 2019, Page(s) 3369-3375, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.9b01050

Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress

Author(s): Lulu Jiang, Pijush Chakraborty , Lushuang Zhang , Melissa Wong, Shannon E Hill, Chelsea Joy Webber , Jenna Libera, Laura J Blair, Benjamin Wolozin , Markus Zweckstetter
Published in: Science Advances, 2023, ISSN 2375-2548
Publisher: American Association for the Advancement of Science’s
DOI: 10.1126/sciadv.add9789

Hsp multichaperone complex buffers pathologically modified Tau.

Author(s): Antonia Moll; Lisa Marie Ramirez; Momchil Ninov; Juliane Schwarz; Henning Urlaub; Markus Zweckstetter
Published in: Nature Communications, Issue 1, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-31396-z

Molecular Basis of Small-Molecule Binding to α-Synuclein

Author(s): Paul Robustelli; Alain Ibanez-de-Opakua; Cecily Campbell-Bezat; Fabrizio Giordanetto; Stefan Becker; Markus Zweckstetter; Albert C. Pan; David E. Shaw
Published in: Crossref, Issue 1, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.1c07591

Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR

Author(s): Christian F Pantoja, Alain Ibáñez de Opakua, Maria-Sol Cima-Omori, Markus Zweckstetter
Published in: Angewandte Chemie International Edition, 2023, ISSN 0044-8249
Publisher: Wiley-VCH
DOI: 10.1002/anie.202218078

Die krankheitsassoziierte Tau‐Phosphorylierung behindert die Tubulinpolymerisation in Tau‐Kondensaten

Author(s): Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Published in: Angewandte Chemie, Issue 133/2, 2021, Page(s) 737-741, ISSN 0044-8249
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202011157

Molecular basis of the interaction of Hsp90 with its co‐chaperone Hop

Author(s): Antonia Lott, Javier Oroz, Markus Zweckstetter
Published in: Protein Science, Issue 29/12, 2020, Page(s) 2422-2432, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3969

Local and Global Dynamics in Intrinsically Disordered Synuclein

Author(s): Nasrollah Rezaei‐Ghaleh, Giacomo Parigi, Andrea Soranno, Andrea Holla, Stefan Becker, Benjamin Schuler, Claudio Luchinat, Markus Zweckstetter
Published in: Angewandte Chemie International Edition, Issue 57/46, 2018, Page(s) 15262-15266, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201808172