Nuclear magnetic resonance spectroscopy of liquid-liquid phase separation

Informazioni relative al progetto

LLPS-NMR

ID dell’accordo di sovvenzione: 787679

DOI

10.3030/787679

Progetto chiuso

Data della firma CE 17 Aprile 2018

Data di avvio 1 Settembre 2018

Data di completamento 31 Agosto 2024

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 460 278,00

Contributo UE

€ 2 460 278,00

Coordinato da

DEUTSCHES ZENTRUM FUR NEURODEGENERATIVE ERKRANKUNGEN EV
Germany

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (42)

A NAC domain mutation (E83Q) unlocks the pathogenicity of human alpha-synuclein and recapitulates its pathological diversity

Autori: Senthil T. Kumar; Anne-Laure Mahul-Mellier; Ramanath Narayana Hegde; Gwladys Rivière; Rani Moons; Alain Ibáñez de Opakua; Pedro Magalhães; Iman Rostami; Sonia Donzelli; Frank Sobott; Markus Zweckstetter; Hilal A. Lashuel
Pubblicato in: Science Advances, Numero 1, 2022, ISSN 2375-2548
Editore: American Association for the Advancement of Science
DOI: 10.1126/sciadv.abn0044

Driving forces behind phase separation of the carboxy-terminal domain of RNA polymerase II

Autori: David Flores-Solis, Irina P. Lushpinskaia, Anton A. Polyansky, Arya Changiarath, Marc Boehning, Milana Mirkovic, James Walshe, Lisa M. Pietrek, Patrick Cramer, Lukas S. Stelzl, Bojan Zagrovic, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-41633-8

Proteasomal degradation of the intrinsically disordered protein tau at single-residue resolution

Autori: T. Ukmar-Godec, P. Fang, A. Ibáñez de Opakua, F. Henneberg, A. Godec, K.-T. Pan, M.-S. Cima-Omori, A. Chari, E. Mandelkow, H. Urlaub, M. Zweckstetter
Pubblicato in: Science Advances, Numero 6/30, 2020, Pagina/e eaba3916, ISSN 2375-2548
Editore: American Association for the Advancement of Science
DOI: 10.1126/sciadv.aba3916

Solid-phase fluorescent BODIPY–peptide synthesis via in situ dipyrrin construction

Autori: Yue Wu, Wing-Sze Tam, Ho-Fai Chau, Simranjeet Kaur, Waygen Thor, Wei Shen Aik, Wai-Lun Chan, Markus Zweckstetter, Ka-Leung Wong
Pubblicato in: Chemical Science, Numero 11/41, 2020, Pagina/e 11266-11273, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d0sc04849f

Contributions of the N‐terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA‐induced phase separation

Autori: Milan Zachrdla; Adriana Savastano; Alain Ibáñez de Opakua; Maria‐Sol Cima‐Omori; Markus Zweckstetter
Pubblicato in: Protein Science, Numero 1, 2022, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4409

Disease‐Associated Tau Phosphorylation Hinders Tubulin Assembly within Tau Condensates

Autori: Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Pubblicato in: Angewandte Chemie International Edition, Numero 60/2, 2021, Pagina/e 726-730, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202011157

Impact of distinct FG nucleoporin repeats on Nup98 self-association

Autori: Alain Ibáñez de Opakua, Christian F. Pantoja, Maria-Sol Cima-Omori, Christian Dienemann, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-48194-4

Condensed-phase signaling can expand kinase specificity and respond to macromolecular crowding

Autori: Dajun Sang; Tong Shu; Christian F. Pantoja; Alain Ibáñez de Opakua; Markus Zweckstetter; Liam J. Holt
Pubblicato in: Molecular Cell, Numero 1, 2022, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2022.08.016

Global Structure of the Intrinsically Disordered Protein Tau Emerges from its Local Structure

Autori: Lukas S. Stelzl; Lisa M. Pietrek; A. Holla; J. S. Oroz; M. Sikora; J. Köfinger; Benjamin Schuler; Markus Zweckstetter; Gerhard Hummer
Pubblicato in: JACS Au, Numero 1, 2022, ISSN 2691-3704
Editore: American Chemical Society
DOI: 10.1101/2021.11.23.469691

Phase Separation in Biology and Disease; Current Perspectives and Open Questions

Autori: Steven Boeynaems, Shasha Chong, Jörg Gsponer, Liam Holt, Dragomir Milovanovic, Diana M Mitrea, Oliver Mueller-Cajar, Bede Portz, John F Reilly, Christopher D Reinkemeier, Benjamin R Sabari, Serena Sanulli, James Shorter, Emily Sontag, Lucia Strader, Jeanne Stachowiak, Stephanie C Weber, Michael White, Huaiying Zhang, Markus Zweckstetter, Shana Elbaum-Garfinkle, Richard Kriwacki
Pubblicato in: Journal of Molecular Biology, 2023, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2023.167971

Multivalent Tau/PSD-95 interactions arrest in vitro condensates and clusters mimicking the postsynaptic density

Autori: Zheng Shen, Daxiao Sun, Adriana Savastano, Sára Joana Varga, Maria-Sol Cima-Omori, Stefan Becker, Alf Honigmann, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-42295-2

Dynamic Aha1 co‐chaperone binding to human Hsp90

Autori: Javier Oroz, Laura J. Blair, Markus Zweckstetter
Pubblicato in: Protein Science, Numero 28/9, 2019, Pagina/e 1545-1551, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3678

Structure and pro-toxic mechanism of the human Hsp90/PPIase/Tau complex

Autori: Javier Oroz, Bliss J. Chang, Piotr Wysoczanski, Chung-Tien Lee, Ángel Pérez-Lara, Pijush Chakraborty, Romina V. Hofele, Jeremy D. Baker, Laura J. Blair, Jacek Biernat, Henning Urlaub, Eckhard Mandelkow, Chad A. Dickey, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06880-0

Multimodal interactions drive chromatin phase separation and compaction

Autori: Tina Ukmar-Godec, Maria-Sol Cima-Omori, Zhadyra Yerkesh, Karthik Eswara, Taekyung Yu, Reshma Ramesh, Gwladys Riviere, Alain Ibanez de Opakua, Wolfgang Fischle, Markus Zweckstetter
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 120, 2024, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2308858120

Alternative low-populated conformations prompt phase transitions in polyalanine repeat expansions

Autori: Rosa Antón, Miguel Á. Treviño, David Pantoja-Uceda, Sara Félix, María Babu, Eurico J. Cabrita, Markus Zweckstetter, Philip Tinnefeld, Andrés M. Vera, Javier Oroz
Pubblicato in: Nature Communications, Numero 15, 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-024-46236-5

Liquid–liquid phase separation of tau: From molecular biophysics to physiology and disease

Autori: Sandeep K. Rai, Adriana Savastano, Priyanka Singh, Samrat Mukhopadhyay, Markus Zweckstetter
Pubblicato in: Protein Science, 2021, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4093

Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils

Autori: Pijush Chakraborty, Gwladys Rivière, Shu Liu, Alain Ibáñez de Opakua, Rıza Dervişoğlu, Alina Hebestreit, Loren B. Andreas, Ina M. Vorberg, Markus Zweckstetter
Pubblicato in: Nature Communications, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-24362-8

Lysine/RNA-interactions drive and regulate biomolecular condensation

Autori: Tina Ukmar-Godec, Saskia Hutten, Matthew P. Grieshop, Nasrollah Rezaei-Ghaleh, Maria-Sol Cima-Omori, Jacek Biernat, Eckhard Mandelkow, Johannes Söding, Dorothee Dormann, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-10792-y

Aromaticity at position 39 in α‐synuclein: A modulator of amyloid fibril assembly and membrane‐bound conformations

Autori: Fiamma A. Buratti; Nicola Boeffinger; Hugo A. Garro; Jesica S. Flores; Francisco J. Hita; Phelippe do Carmo Gonçalves; Federico dos Reis Copello; Leonardo Lizarraga; Giulia Rossetti; Paolo Carloni; Markus Zweckstetter; Tiago F. Outeiro; Stefan Eimer; Christian Griesinger; Claudio O. Fernández
Pubblicato in: Protein science 31(7), e4360 (2022). doi:10.1002/pro.4360, Numero 1, 2022, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4360

Potent Tau aggregation inhibitor D-peptides selected against Tau-repeat 2 using mirror image phage display

Autori: Marwa Nidal Malhis; Senthilvelrajan Kaniyappan; Isabelle Aillaud; Ram Reddy Chandupatla; Lisa Marie Ramirez; Markus Zweckstetter; Anselm H. C. Horn; Anselm H. C. Horn; Eckhard Mandelkow; Heinrich Sticht; Susanne Aileen Funke
Pubblicato in: ChemBioChem, Numero 1, 2021, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202100287

NMR hawk-eyed view of AlphaFold2 structures.

Autori: Markus Zweckstetter; Markus Zweckstetter
Pubblicato in: Protein Science : A Publication of the Protein Society, Numero 1, 2021, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4175

Peptidyl Prolyl Isomerase A Modulates the Liquid–Liquid Phase Separation of Proline-Rich IDPs

Autori: Maria Babu; Filippo Favretto; Marija Rankovic; Markus Zweckstetter
Pubblicato in: Journal of the American Chemical Society, Numero 1, 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.2c07149

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

Autori: Adriana Savastano, Alain Ibáñez de Opakua, Marija Rankovic, Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 11/1, 2020, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-020-19843-1

Methylene blue accelerates liquid-to-gel transition of tau condensates impacting tau function and pathology

Autori: Yongqi Huang, Jitao Wen, Lisa-Marie Ramirez, Eymen Gümüşdil, Pravin Pokhrel, Viet H. Man, Haiqiong Ye, Yue Han, Yunfei Liu, Ping Li, Zhengding Su, Junmei Wang, Hanbin Mao, Markus Zweckstetter, Sarah Perrett, Si Wu, Meng Gao
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-41241-6

Interplay between tau and α-synuclein liquid-liquid phase separation.

Autori: Anna Siegert; Marija Rankovic; Filippo Favretto; Tina Ukmar-Godec; Timo Strohäker; Stefan Becker; Markus Zweckstetter; Markus Zweckstetter
Pubblicato in: Protein Science : A Publication of the Protein Society, Numero 1, 2021, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.4025

Solid-state NMR investigation of the involvement of the P2 region in tau amyloid fibrils

Autori: Savastano, Adriana; Jaipuria, Garima; Andreas, Loren; Mandelkow, Eckhard; Zweckstetter, Markus
Pubblicato in: Crossref, Numero 1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-78161-0

The pathogenic R5L mutation disrupts formation of Tau complexes on the microtubule by altering local N-terminal structure

Autori: Alisa Cario, Adriana Savastano, Neil B. Wood, Zhu Liu,Michael J. Previs, Adam G. Hendricks, Markus Zweckstetter, Christopher L. Berger
Pubblicato in: Proceedings of the National Academy of Sciences, 2022, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.2114215119

Quaternary structure of patient-homogenate amplified α-synuclein fibrils modulates seeding of endogenous α-synuclein

Autori: Benedikt Frieg; James A. Geraets; Timo Strohäker; Christian Dienemann; Panagiota Mavroeidi; Byung Chul Jung; Woojin S. Kim; Seung-Jae Lee; Maria Xilouri; Markus Zweckstetter; Gunnar F. Schröder
Pubblicato in: Communications biology 5(1), 1040 (2022). doi:10.1038/s42003-022-03948-y, Numero 1, 2022, ISSN 2399-3642
Editore: Springer
DOI: 10.1038/s42003-022-03948-y

Molecular interactions of FG nucleoporin repeats at high resolution

Autori: Alain Ibáñez de Opakua, James A Geraets, Benedikt Frieg, Christian Dienemann, Adriana Savastano, Marija Rankovic, Maria-Sol Cima-Omori, Gunnar F Schröder, Markus Zweckstetter
Pubblicato in: Nature Chemistry, 2022, ISSN 1755-4330
Editore: Nature Publishing Group
DOI: 10.1038/s41557-022-01035-7

Residue-specific identification of phase separation hot spots of Alzheimer's-related protein tau

Autori: Susmitha Ambadipudi, Jithender G. Reddy, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Pubblicato in: Chemical Science, Numero 10/26, 2019, Pagina/e 6503-6507, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/c9sc00531e

Biomolecular condensation of the microtubule-associated protein tau

Autori: Tina Ukmar-Godec, Susanne Wegmann, Markus Zweckstetter
Pubblicato in: Seminars in Cell & Developmental Biology, Numero 99, 2020, Pagina/e 202-214, ISSN 1084-9521
Editore: Academic Press
DOI: 10.1016/j.semcdb.2019.06.007

RNA polymerase II clustering through carboxy-terminal domain phase separation

Autori: Marc Boehning, Claire Dugast-Darzacq, Marija Rankovic, Anders S. Hansen, Taekyung Yu, Herve Marie-Nelly, David T. McSwiggen, Goran Kokic, Gina M. Dailey, Patrick Cramer, Xavier Darzacq, Markus Zweckstetter
Pubblicato in: Nature Structural & Molecular Biology, Numero 25/9, 2018, Pagina/e 833-840, ISSN 1545-9993
Editore: Nature Publishing Group
DOI: 10.1038/s41594-018-0112-y

Conformational Dynamics of Intrinsically Disordered Proteins Regulate Biomolecular Condensate Chemistry.

Autori: Anton Abyzov; Martin Blackledge; Markus Zweckstetter
Pubblicato in: Crossref, Numero 1, 2022, ISSN 0009-2665
Editore: American Chemical Society
DOI: 10.1021/acs.chemrev.1c00774

Contributions of the N-terminal intrinsically disordered region of the SARS-CoV-2 nucleocapsid protein to RNA-induced phase separation

Autori: Milan Zachrdla, Adriana Savastano, Alain Ibáñez de Opakua, Maria-Sol Cima-Omori, Markus Zweckstetter
Pubblicato in: Protein Science, 2022, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1101/2022.03.30.486418

Reorientational Dynamics of Amyloid-β from NMR Spin Relaxation and Molecular Simulation

Autori: Nasrollah Rezaei-Ghaleh, Giacomo Parigi, Markus Zweckstetter
Pubblicato in: The Journal of Physical Chemistry Letters, Numero 10/12, 2019, Pagina/e 3369-3375, ISSN 1948-7185
Editore: American Chemical Society
DOI: 10.1021/acs.jpclett.9b01050

Chaperoning of specific tau structure by immunophilin FKBP12 regulates the neuronal resilience to extracellular stress

Autori: Lulu Jiang, Pijush Chakraborty , Lushuang Zhang , Melissa Wong, Shannon E Hill, Chelsea Joy Webber , Jenna Libera, Laura J Blair, Benjamin Wolozin , Markus Zweckstetter
Pubblicato in: Science Advances, 2023, ISSN 2375-2548
Editore: American Association for the Advancement of Science’s
DOI: 10.1126/sciadv.add9789

Hsp multichaperone complex buffers pathologically modified Tau.

Autori: Antonia Moll; Lisa Marie Ramirez; Momchil Ninov; Juliane Schwarz; Henning Urlaub; Markus Zweckstetter
Pubblicato in: Nature Communications, Numero 1, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-31396-z

Molecular Basis of Small-Molecule Binding to α-Synuclein

Autori: Paul Robustelli; Alain Ibanez-de-Opakua; Cecily Campbell-Bezat; Fabrizio Giordanetto; Stefan Becker; Markus Zweckstetter; Albert C. Pan; David E. Shaw
Pubblicato in: Crossref, Numero 1, 2022, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.1c07591

Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR

Autori: Christian F Pantoja, Alain Ibáñez de Opakua, Maria-Sol Cima-Omori, Markus Zweckstetter
Pubblicato in: Angewandte Chemie International Edition, 2023, ISSN 0044-8249
Editore: Wiley-VCH
DOI: 10.1002/anie.202218078

Die krankheitsassoziierte Tau‐Phosphorylierung behindert die Tubulinpolymerisation in Tau‐Kondensaten

Autori: Adriana Savastano, David Flores, Harindranath Kadavath, Jacek Biernat, Eckhard Mandelkow, Markus Zweckstetter
Pubblicato in: Angewandte Chemie, Numero 133/2, 2021, Pagina/e 737-741, ISSN 0044-8249
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202011157

Molecular basis of the interaction of Hsp90 with its co‐chaperone Hop

Autori: Antonia Lott, Javier Oroz, Markus Zweckstetter
Pubblicato in: Protein Science, Numero 29/12, 2020, Pagina/e 2422-2432, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3969

Local and Global Dynamics in Intrinsically Disordered Synuclein

Autori: Nasrollah Rezaei‐Ghaleh, Giacomo Parigi, Andrea Soranno, Andrea Holla, Stefan Becker, Benjamin Schuler, Claudio Luchinat, Markus Zweckstetter
Pubblicato in: Angewandte Chemie International Edition, Numero 57/46, 2018, Pagina/e 15262-15266, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201808172