Objective
Human cells display a staggering repertoire of different sialic acid-carrying glycans (sialoglycans) at the surface - the Sialome. The Sialome regulates numerous biological processes including cell communication and migration and is involved in diseases such as infections , autoimmunity and cancer. However, most biological interactions and functions of the Sialome remain elusive, because suitable methods to address the Sialome as an ‘Ome’ and to dissect specific sialoglycan functions in the cell context are lacking. Therefore, I propose to generate a cell-based sialoglycan array for the display of all human sialoglycans in the natural context of individual glycoconjugates at the cell surface in an arrayable format. Using precision genome editing (CRISPR/Cas9) genes involved in sialoglycan synthesis will be targeted to create a library of isogenic human cells each displaying a unique part of the Sialome. These isogenic cells together form the cell-based sialoglycan array and will be used for high-throughput screening and discovery of binding specificities of sialoglycan-binding proteins such as Siglecs, selectins and factor H. Finally, the cell-based sialoglycan array will be used for the production of a representative panel of recombinant glycoproteins carrying homogenous N-linked or O-linked sialoglycans. The proposed research will take place at the at the Copenhagen Center for Glycomics a world leader in Glycomics and pioneer in precision genome editing in Glycobiology where I will be trained in precision genome editing, glycoengineering and state-of-the-art glycan mass spectrometry. Altogether, the cell-based sialoglycan array will be indefinitely sustainable, addressable by any multiplex assay, and serve as production platform for recombinant sialoglycoproteins. Ultimately, I expect that the proposed cell-based sialoglycan array will open the door for wide discovery of biological interactions and functions and of the human Sialome.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2017
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1165 KOBENHAVN
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.