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Directed Evolution of Novel Artificial Metalloenzyme Platforms for Biocatalysis

Objective

Artificial metalloenzymes have emerge as a fusion of synthetic and biological catalysts for environmentally friendly synthesis. These hybrid catalysts are created by tethering a catalytic metal ion or cofactor to a protein scaffold. Optimized artificial metalloenzymes have the potential to harness the advantages of both systems, inheriting the versatility of synthetic catalysts and the efficiency and robustness of enzymes. New tools, however, are required to realize the potential of artificial metalloenzymes. The objective of the ArtMetBio project is to create novel platforms for artificial metalloenzymes that optimize catalytic performance and versatility via increased cooperation between the synthetic and biological components.

Now, more than ever, green catalysis is a crucial global endeavor. Our growing ability to evolve enzymes enables the use of new, unexpected protein scaffolds, opening the door to new innovative platforms. Taking advantage of directed evolution methods, this proposal outlines a strategy for repurposing two well characterized protein scaffolds to create artificial metalloenzymes for in vivo metathesis, a canonical reaction in synthetic chemistry not found in nature. Aim 1 of the project is to optimize metathesis-catalyzing enzymes, coined metathases, through directed evolution. Aim 2 will apply these metathases to engineer a biocatalytic cascade for in vivo synthesis of high-value privileged compounds. The proposed systems differ from the current state-of-the art—streptavidin and heme artificial metalloenzymes—because they are monomeric, non-toxic, and highly modular, enabling wide catalytic versatility. The proposed action has the potential to provide unique, versatile tools for synthesis and biocatalysis.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2017

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Coordinator

UNIVERSITAT BASEL
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 175 419,60
Address
PETERSPLATZ 1
4051 Basel
Switzerland

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Region
Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Stadt
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 175 419,60
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