Objective
Wolbachia is a widespread, intracellular symbiont of arthropods and filarial nematodes, inducing reproductive distortions and antiviral protection in insects. Wolbachia is currently being deployed in disease vector control programs and is also a target in the treatment of lymphatic filariasis. Despite its prevalence, fascinating phenotypes, and applied importance, Wolbachia biology remains poorly explored, as it cannot be cultured or genetically manipulated. To date, antisense RNA and a transfection reagent that knock down Wolbachia gene expression have been deployed in cell culture, but the effect was modest and likely very transient. In order to achieve a robust and lasting antisense inhibition in Wolbachia, applicable in an in vivo system, I will utilize developments from drug delivery and gene manipulation in other members of the Rickettsiales. This will involve the use of nuclease resistant nucleic acid analogs, which can strongly inhibit transcripts for many days. This technology will be combined with attachment to different transporter molecules, known to deliver nucleic acids to other intracellular bacteria and parasites within host cells and organisms. I will then use this system to interrogate the genes putatively involved in host-microbe interactions in cell culture. I will focus on genes underlying symbiosis and those directly conferring the trait of antiviral resistance. The technology created will be widely applicable in studies of host-symbiont interactions, and in determining the mechanisms underlying the diverse phenotypes and symbioses observed. This project will thus both enable discovery science and allow better human disease prevention and treatment.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences biological sciences zoology entomology
- medical and health sciences basic medicine pharmacology and pharmacy drug resistance multidrug resistance
- natural sciences biological sciences zoology invertebrate zoology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2017
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
L69 7ZX LIVERPOOL
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.