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Deciphering of Root and Rhizosphere microbiome to increase host fitness in the Fusarium oxysporum- plant interaction

Objective

Fungi have a devastating impact on human nutrition and health. Fungal pathogens provoke huge yield losses in crops, destroying around 23% of the agricultural production annually. A major proportion of this loss is caused by pathogenic root-colonizing microbes that have evolved sophisticated strategies to outcompete the benign microbial competitors in the rhizosphere and to actively suppress the plant immune response. The fungal pathogen Fusarium oxysporum (Fox) is a soil-borne root infecting fungus that provokes vascular wilt disease in over a hundred different agricultural crops, and represents a serious threat world-wide. The current control methods for this pathogen depend upon extensive use of chemical pesticides, which are highly unsustainable in the food-chain. Here we propose to use the Fox-tomato interaction as a model to molecularly dissect the role of the root (including endophytic) and rhizosphere microbiota in modulating vascular wilt disease. We will use a barcoded pyrosequencing approach to taxonomically define the root and rhizosphere microbiome in tomato. Next, we will dissect the impact of the microbiome on fungal host sensing, using an established collection of isogenic signalling mutants of Fox available in the host lab. We will carry out in vivo Fox- bacterial interaction assays to generate a dataset for identification of bacterial communities exerting an antagonistic effect on pathogen colonization. The project will thus produce new insights into the interplay between the root microbiome and pathogen colonization. The, generated knowledge platform will be used to reassemble a synthetic ‘SynCom’ microbiome that will be used to control Vascular Wilt, thereby advancing the development of sustainable agriculture.

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-CAR - CAR – Career Restart panel

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2017

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Coordinator

UNIVERSIDAD DE CORDOBA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 170 121,60
Address
AVENIDA DE MEDINA AZAHARA 5
14005 CORDOBA
Spain

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Region
Sur Andalucía Córdoba
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 170 121,60
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