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New Super Resolution microscopy solution for living cell imaging

Periodic Reporting for period 2 - LiveCODIM (New Super Resolution microscopy solution for living cell imaging)

Reporting period: 2019-07-01 to 2020-10-31

In the last years, major advances in imaging have significantly contributed to the improvement of EU citizens’ quality of life, from research to diagnostic and therapy. Super Resolution microscopy (SR) is a milestone discovery, which goes beyond the limits of optics, typically around 250nm, or ¼ of a μm (one can see inside a virus!). Nevertheless, SR has yet to become a standard imaging modality, like the confocal or the widefield microscopes. Dissemination is still slowed by three critical challenges:
• Damage to the biological tissue – it’s almost impossible to observe live cells
• Technical complexity – only highly skilled operators can use the instruments
• Cost of ownership – typically around half million euros for a complete solution.

The project LiveCODIM by BioAxial is leveraging the CODIM family of SR microscopes, using proprietary and well-patented technology.

The general objectives of this project aim at scaling up SR microscopy, with a breakthrough solution:
• Specifically designed for the study of living cells
• A plug and play system requiring neither extensive, multi-skill staff training nor complex sample preparation
• 3x more cost-effective than the few other comparable solutions, with higher resolution
• Turning any fluorescence microscope into the latest generation SR system.
The first project period focused on:
• The validation of the LiveCODIM design against user requirements
• Developing the control system and acquisition module
• Validating the hardware module
• Improving the software architecture and reconstruction algorithms

Measurable super resolution results have been obtained with various sample types, meeting target specifications. The LiveCODIM project is on track with many challenges remaining to be addressed during the second project period.
Not just an evolution, but also a genuine revolution, LiveCODIM initially addresses the life science imaging market, a 1Bn€ playground, and has many other potential applications, in diagnostic (human tissue) and outside life science.
This project brings multiple benefits to EU citizens, by its contribution to life and environmental improvement.

The second reporting period will focus on the obtaining feedback of the LiveCODIM system developed so far, improvements, deployment and successful dissemination into the life science community.
The LiveCODIM add-on module installed on a widefield microscope