Dissecting and Overcoming Innate Immune Barriers for Therapeutically Efficient Hematopoietic Stem Cell Gene Engineering

Project Information

ImmunoStem

Grant agreement ID: 819815

DOI

10.3030/819815

Project closed

EC signature date 18 February 2019

Start date 1 March 2019

End date 28 February 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 994 375,00

EU contribution

€ 1 994 375,00

Coordinated by

UNIVERSITA DEGLI STUDI DI PAVIA
Italy

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Non-peer reviewed articles (3)

AAV vectors trigger DNA damage responses and STING-dependent inflammation in human CNS cells

Author(s): Helena Costa-Verdera, Vasco Meneghini, Zachary Fitzpatrick, Monah Abou Alezz, Emily Fabyanic, Xin Huang, Yulia Dzhashiashvili, Avantika Ahiya, Elisabeth Mangiameli, Erika Valeri, Giovanni Crivicich, Ivan Cuccovillo, Roberta Caccia, Berangere Bertin, Giuseppe Ronzitti, Esteban Engel, Ivan Merelli, Federico Mingozzi, Angela Gritti, Klaudia Kuranda, Anna Kajaste-Rudnitski
Published in: Research square, 2024, ISSN 2693-5015
Publisher: Research Square Company
DOI: 10.21203/rs.3.rs-4171795/v1

Antiviral immunity lassoed down by excess RNA

Author(s): Erika Valeri, Anna Kajaste-Rudnitski
Published in: Journal of Experimental Medicine, Issue 222, 2024, ISSN 0022-1007
Publisher: Rockefeller University Press
DOI: 10.1084/jem.20241743

The lysine-rich intracellular loop and cell type-specific co-factors are required for IFITM3 antiviral immunity in hematopoietic stem cells

Author(s): G. Unali, A.M.S Giordano, I. Cuccovillo, Abou Alezz M., L. Apolonia, I. Merelli, M. H. Malim, C. Petrillo, A. Kajaste-Rudnitski
Published in: bioRxiv, 2021, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratories
DOI: 10.1101/2021.04.06.438585

Peer reviewed articles (8)

Antiviral immunity and nucleic acid sensing in haematopoietic stem cell gene engineering

Author(s): Francesco Piras, Anna Kajaste-Rudnitski
Published in: Gene Therapy, 2020, ISSN 0969-7128
Publisher: Nature Publishing Group
DOI: 10.1038/s41434-020-0175-3

AAV vectors trigger DNA damage response-dependent pro-inflammatory signalling in human iPSC-derived CNS models and mouse brain

Author(s): Helena Costa-Verdera, Vasco Meneghini, Zachary Fitzpatrick, Monah Abou Alezz, Emily Fabyanic, Xin Huang, Yulia Dzhashiashvili, Avantika Ahiya, Elisabeth Mangiameli, Erika Valeri, Giovanni Crivicich, Silvia Piccolo, Ivan Cuccovillo, Roberta Caccia, Ying Kai Chan, Bérangère Bertin, Giuseppe Ronzitti, Esteban A. Engel, Ivan Merelli, Federico Mingozzi, Angela Gritti, Klaudia Kuranda, Anna Kajaste-Ru
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-58778-3

A novel STING variant triggers endothelial toxicity and SAVI disease

Author(s): Erika Valeri, Sara Breggion, Federica Barzaghi, Monah Abou Alezz, Giovanni Crivicich, Isabel Pagani, Federico Forneris, Claudia Sartirana, Matteo Costantini, Stefania Costi, Achille Marino, Eleonora Chiarotto, Davide Colavito, Rolando Cimaz, Ivan Merelli, Elisa Vicenzi, Alessandro Aiuti, Anna Kajaste-Rudnitski
Published in: Journal of Experimental Medicine, Issue 221, 2024, ISSN 0022-1007
Publisher: Rockefeller University Press
DOI: 10.1084/jem.20232167

Laboratory-scale lentiviral vector production and purification for enhanced ex vivo and in vivo genetic engineering

Author(s): Monica Soldi, Lucia Sergi Sergi, Giulia Unali, Thomas Kerzel, Ivan Cuccovillo, Paola Capasso, Andrea Annoni, Mauro Biffi, Paola Maria Vittoria Rancoita, Alessio Cantore, Angelo Lombardo, Luigi Naldini, Mario Leonardo Squadrito, Anna Kajaste-Rudnitski
Published in: Molecular Therapy - Methods & Clinical Development, 2020, ISSN 2329-0501
Publisher: Cell Press
DOI: 10.1016/j.omtm.2020.10.009

Interferon‐inducible phospholipids govern <scp>IFITM3</scp>‐dependent endosomal antiviral immunity

Author(s): Giulia Unali, Giovanni Crivicich, Isabel Pagani, Monah Abou‐Alezz, Filippo Folchini, Erika Valeri, Vittoria Matafora, Julie A Reisz, Anna Maria Sole Giordano, Ivan Cuccovillo, Giacomo M Butta, Lorena Donnici, Angelo D'Alessandro, Raffaele De Francesco, Lara Manganaro, Davide Cittaro, Ivan Merelli, Carolina Petrillo, Angela Bachi, Elisa Vicenzi, Anna Kajaste‐Rudnitski
Published in: The EMBO Journal, Issue 42, 2023, ISSN 0261-4189
Publisher: Nature Publishing Group
DOI: 10.15252/embj.2022112234

Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cells

Author(s): Samuele Ferrari, Aurelien Jacob, Daniela Cesana, Marianne Laugel, Stefano Beretta, Angelica Varesi, Giulia Unali, Anastasia Conti, Daniele Canarutto, Luisa Albano, Andrea Calabria, Valentina Vavassori, Carlo Cipriani, Maria Carmina Castiello, Simona Esposito, Chiara Brombin, Federica Cugnata, Oumeya Adjali, Eduard Ayuso, Ivan Merelli, Anna Villa, Raffaella Di Micco, Anna Kajaste-Rudnitski, Eugenio
Published in: Cell Stem Cell, Issue 29, 2022, Page(s) 1428-1444.e9, ISSN 1934-5909
Publisher: Cell Press
DOI: 10.1016/j.stem.2022.09.001

Removal of innate immune barriers allows efficient transduction of quiescent human hematopoietic stem cells

Author(s): Erika Valeri, Giulia Unali, Francesco Piras, Monah Abou-Alezz, Giulia Pais, Fabrizio Benedicenti, Maria Rosa Lidonnici, Ivan Cuccovillo, Ilaria Castiglioni, Sergio Arévalo, Giulio Spinozzi, Ivan Merelli, Rayk Behrendt, Adrian Oo, Baek Kim, Nathaniel R. Landau, Giuliana Ferrari, Eugenio Montini, Anna Kajaste-Rudnitski
Published in: Molecular Therapy, Issue 32, 2024, Page(s) 124-139, ISSN 1525-0016
Publisher: Nature Publishing Group
DOI: 10.1016/j.ymthe.2023.11.020

Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking

Author(s): Samuele Ferrari, Aurelien Jacob, Stefano Beretta, Giulia Unali, Luisa Albano, Valentina Vavassori, Davide Cittaro, Dejan Lazarevic, Chiara Brombin, Federica Cugnata, Anna Kajaste-Rudnitski, Ivan Merelli, Pietro Genovese, Luigi Naldini
Published in: Nature Biotechnology, 2020, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-020-0551-y