Microbial genome defence pathways: from molecular mechanisms to next-generation molecular tools

Project Information

CRISPR2.0

Grant agreement ID: 820152

DOI

10.3030/820152

Project closed

EC signature date 7 March 2019

Start date 1 May 2019

End date 31 October 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 996 525,00

EU contribution

€ 1 996 525,00

Coordinated by

UNIVERSITAT ZURICH
Switzerland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (12)

Past, present, and future of CRISPR genome editing technologies

Author(s): Martin Pacesa, Oana Pelea, Martin Jinek
Published in: Cell, Issue 187, 2024, Page(s) 1076-1100, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2024.01.042

Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA

Author(s): Giada Finocchio; Balwina Koopal; Ana Potocnik; Clint Heijstek; Adrie H Westphal; Martin Jinek; Daan C Swarts
Published in: Nucleic Acids Research, 2024, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad1248

Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor

Author(s): Kristina Makasheva; Louise C. Bryan; Carolin Anders; Sherin Panikulam; Martin Jinek; Beat Fierz
Published in: Journal of the American Chemical Society, Issue 143, 2021, Page(s) 16313–16319, ISSN 1520-5126
Publisher: American Chemical Society
DOI: 10.5167/uzh-210326

DNA end sensing and cleavage by the Shedu anti-phage defense system

Author(s): Luuk Loeff, Alexander Walter, Gian Tizio Rosalen, Martin Jinek
Published in: Cell, Issue 3, 2025, Page(s) 721-733, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1101/2023.08.10.552762

Substrate selectivity and catalytic activation of the type III CRISPR ancillary nuclease Can2

Author(s): Kenny Jungfer, Annina Sigg, Martin Jinek
Published in: Nucleic Acids Research, 2024, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad1102

Molecular mechanism of plasmid elimination by the DdmDE defense system

Author(s): Luuk Loeff, David W. Adams, Christelle Chanez, Sandrine Stutzmann, Laurie Righi, Melanie Blokesch, Martin Jinek
Published in: Science, Issue 385, 2024, Page(s) 188-194, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.adq0534

Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation

Author(s): Luuk Loeff, Alena Kroupova, Igor Asanović, Franziska M. Boneberg, Moritz M. Pfleiderer, Luca Riermeier, Alexander Leitner, Andrè Ferdigg, Fabian Ackle, Javier Martinez, Martin Jinek
Published in: Nature Structural & Molecular Biology, Issue 32, 2025, Page(s) 1205-1212, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-025-01516-6

Structural basis for the assembly of the type V CRISPR-associated transposon complex

Author(s): Michael Schmitz, Irma Querques, Seraina Oberli, Christelle Chanez, Martin Jinek
Published in: Cell, Issue 185, 2022, Page(s) 4999-5010, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2022.11.009

Mechanistic determinants and dynamics of cA6 synthesis in type III CRISPR-Cas effector complexes

Author(s): Kenny Jungfer, Štefan Moravčík, Carmela Garcia-Doval, Anna Knörlein, Jonathan Hall, Martin Jinek
Published in: Nucleic Acids Research, Issue 53, 2025, Page(s) gkae1277, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkae1277

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

Author(s): Carmela Garcia-Doval, Frank Schwede, Christian Berk, Jakob T. Rostøl, Ole Niewoehner, Oliver Tejero, Jonathan Hall, Luciano A. Marraffini, Martin Jinek
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-15334-5

Target site selection and remodelling by type V CRISPR-transposon systems

Author(s): Irma Querques, Michael Schmitz, Seraina Oberli, Christelle Chanez, Martin Jinek
Published in: Nature, Issue 599, 2021, Page(s) 497-502, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-021-04030-z

Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons

Author(s): Giada Finocchio, Irma Querques, Christelle Chanez, Katarzyna J Speichert, Martin Jinek
Published in: Nucleic Acids Research, Issue 53, 2025, Page(s) gkaf149, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaf149

Other (1)

Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons

Author(s): Michael Schmitz, Irma Querques, Seraina Oberli, Christelle Chanez, Martin Jinek
Published in: 2022
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.06.17.496590