Novel Testing Strategies for Endocrine Disruptors in the Context of Developmental NeuroToxicity

Deliverables

Websites, patent fillings, videos etc. (3)

1st animation

First animation video showing highlights of the project results

2nd animation

Second animation video showing highlights of the project results

Launch of Website, logo and flyer

Documents, reports (1)

Set of test compounds

Final list of the set of chemicals tested in WP2 and WP3.

Publications

Peer reviewed articles (28)

A human stem cell-derived test system for agents modifying neuronal N -methyl- d -aspartate-type glutamate receptor Ca 2+ -signalling

Author(s): Stefanie Klima; Markus Brüll; Anna-Sophie Spreng; Ilinca Suciu; Tjalda Falt; Jens Christian Schwamborn; Tanja Waldmann; Christiaan Karreman; Marcel Leist
Published in: Archives of Toxicology, 6, 2021, ISSN 1432-0738
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03024-0

Human neuronal signaling and communication assays to assess functional neurotoxicity.

Author(s): Dominik Loser; Jasmin Schaefer; Timm Danker; Clemens Möller; Markus Brüll; Ilinca Suciu; Anna-Katharina Ückert; Stefanie Klima; Marcel Leist; Udo Kraushaar
Published in: Archives of Toxicology, 5, 2021, ISSN 1432-0738
Publisher: Springer Verlag
DOI: 10.1007/s00204-020-02956-3

https://www.altex.org/index.php/altex/article/view/1339

Author(s): Alice Krebs, Tanja Waldmann, Martin F. Wilks, Barbara M. A. van Vugt-Lussenburg, Bart van der Burg, Andrea Terron, Thomas Steger-Hartmann, Joelle Ruegg, Costanza Rovida, Emma Pedersen, Giorgia Pallocca, Mirjam Luijten, Sofia B. Leite, Stefan Kustermann, Hennicke Kamp, Julia Hoeng, Philip Hewitt, Matthias Herzler, Jan G. Hengstler, Tuula Heinonen, Thomas Hartung, Barry Hardy, Florian Gantner, Ellen
Published in: ALTEX, 36(4), 2019, Page(s) 682-699, ISSN 1868-596X
Publisher: Springer-Verlag Gmb
DOI: 10.14573/altex.1909271

CaFFEE: A program for evaluating time courses of Ca2+ dependent signal changes of complex cells loaded with fluorescent indicator dyes

Author(s): Christiaan Karreman
Published in: ALTEX, 37(2), 2020, Page(s) 332-336, ISSN 1868-596X
Publisher: Springer-Verlag GmbH
DOI: 10.14573/altex.2003191

Acute effects of the imidacloprid metabolite desnitro-imidacloprid on human nACh receptors relevant for neuronal signaling.

Author(s): Dominik Loser; Dominik Loser; Karin Grillberger; Maria G. Hinojosa; Jonathan Blum; Yves Haufe; Timm Danker; Ylva Johansson; Clemens Möller; Annette Nicke; Susanne Hougaard Bennekou; Iain Gardner; Caroline Bauch; Paul Walker; Anna Forsby; Gerhard F. Ecker; Udo Kraushaar; Marcel Leist
Published in: Archives of Toxicology, 7, 2021, ISSN 1432-0738
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03168-z

Environmental influences on brain aging

Author(s): Barbara A. Demeneix
Published in: Aging Brain,Volume 1, 2021, 100003, 7, 2021, ISSN 2589-9589
Publisher: Elsevier
DOI: 10.1016/j.nbas.2020.100003

Determination of benchmark concentrations and their statistical uncertainty for cytotoxicity test data and functional in vitro assays

Author(s): Alice Krebs
Published in: ALTEX, 37(1), 2019, Page(s) 155-163, ISSN 1868-596X
Publisher: Springer -Verlag GmbH
DOI: 10.14573/altex.1912021

Autism spectrum disorder at the crossroad between genes and environment: contributions, convergences, and interactions in ASD developmental pathophysiology

Author(s): Cristina Cheroni; Cristina Cheroni; Nicolò Caporale; Nicolò Caporale; Giuseppe Testa; Giuseppe Testa
Published in: Molecular Autism, Vol 11, Iss 1, Pp 1-18 (2020), 2, 2020, ISSN 2040-2392
Publisher: BioMed Central
DOI: 10.1186/s13229-020-00370-1

Neurotoxicity and underlying cellular changes of 21 mitochondrial respiratory chain inhibitors

Author(s): Johannes Delp; Andrea Cediel-Ulloa; Andrea Cediel-Ulloa; Ilinca Suciu; Petra Kranaster; Barbara M.A. van Vugt-Lussenburg; Vesna Munic Kos; Wanda van der Stel; Giada Carta; Susanne Hougaard Bennekou; Paul Jennings; Bob van de Water; Anna Forsby; Anna Forsby; Marcel Leist
Published in: Delp , J , Cediel-Ulloa , A , Suciu , I , Kranaster , P , van Vugt-Lussenburg , B M , Munic Kos , V , van der Stel , W , Carta , G , Bennekou , S H , Jennings , P , van de Water , B , Forsby , A & Leist , M 2021 , ' Neurotoxicity and underlying cellular changes of 21 mitochondrial respiratory chain inhibitors ' , Archives of Toxicology , vol. 95 , pp. 591–615 . https://doi.org/10.1007/s00204-020, 5, 2021, ISSN 1432-0738
Publisher: Springer
DOI: 10.1007/s00204-020-02970-5

DNA methylation at GRIN2B partially mediates the association between prenatal bisphenol F exposure and cognitive functions in 7-year-old children in the SELMA study

Author(s): Elin Engdahl, Katherine Svensson, Ping-I Daniel Lin, Ali Alavian-Ghavaninic, Christian Lindh, Joëlle Rüegg, Carl-Gustaf Bornehag
Published in: Environment International, Volume 156, November 2021, 106617, 2021, ISSN 0160-4120
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2021.106617

Functional alterations by a subgroup of neonicotinoid pesticides in human dopaminergic neurons.

Author(s): Dominik Loser; Maria G. Hinojosa; Jonathan Blum; Jasmin Schaefer; Markus Brüll; Ylva Johansson; Ilinca Suciu; Karin Grillberger; Timm Danker; Clemens Möller; Iain Gardner; Gerhard F. Ecker; Susanne Hougaard Bennekou; Anna Forsby; Udo Kraushaar; Marcel Leist
Published in: Archives of Toxicology, 1, 2021, ISSN 1432-0738
Publisher: Springer Verlag
DOI: 10.1007/s00204-021-03031-1

Identification of mitochondrial toxicants by combined in silico and in vitro studies : a structure-based view on the Adverse Outcome Pathway

Author(s): Florentina Troger; Johannes Delp; Melina Funke; Wanda van der Stel; Claire Colas; Marcel Leist; Bob van de Water; Gerhard F. Ecker
Published in: Computational Toxicology, 14, 8, 2020, ISSN 2468-1113
Publisher: Elsevier
DOI: 10.1016/j.comtox.2020.100123

Development of a neural rosette formation assay (RoFA) to identify neurodevelopmental toxicants and to characterize their transcriptome disturbances

Author(s): Nadine Dreser, Katrin Madjar, Anna-Katharina Holzer, Marion Kapitza, Christopher Scholz, Petra Kranaster, Simon Gutbier, Stefanie Klima, David Kolb, Christian Dietz, Timo Trefzer, Johannes Meisig, Christoph van Thriel, Margit Henry, Michael R. Berthold, Nils Blüthgen, Agapios Sachinidis, Jörg Rahnenführer, Jan G. Hengstler, Tanja Waldmann, Marcel Leist
Published in: Archives of Toxicology, 94/1, 2020, Page(s) 151-171, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-019-02612-5

Evidenced-Based Approaches to Support the Development of Endocrine-Mediated Adverse Outcome Pathways: Challenges and Opportunities

Author(s): Karine Audouze, Elias Zgheib, Khaled Abass, Asma H. Baig, Isabel Forner-Piquer, Henrik Holbech, Dries Knapen, Pim E. G. Leonards, Diana I. Lupu, Saranya Palaniswamy, Arja Rautio, Maria Sapounidou, Olwenn V. Martin
Published in: Front. Toxicol, 2021, ISSN 2673-3080
Publisher: Frontiers
DOI: 10.3389/ftox.2021.787017

Profiling of Human Neural Crest Chemoattractant Activity as a Replacement of Fetal Bovine Serum for In Vitro Chemotaxis Assays

Author(s): Xenia Dolde; Christiaan Karreman; Marianne Wiechers; Stefan Schildknecht; Marcel Leist
Published in: International Journal of Molecular Sciences, Vol 22, Iss 10079, p 10079 (2021), 9, 2021, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221810079

Novel in vitro Experimental Approaches to Study Myelination and Remyelination in the Central Nervous System

Author(s): Davide Marangon, Nicolò Caporale, Marta Boccazzi, Maria P. Abbracchio, Giuseppe Testa, Davide Lecca
Published in: Frontiers in cellular neuroscience, 2021, ISSN 1662-5102
Publisher: Frontiers Research Foundation
DOI: 10.3389/fncel.2021.748849

Pesticides With Potential Thyroid Hormone-Disrupting Effects: A Review of Recent Data

Author(s): Michelle Leemans, Stephan Couderq, Barbara Demeneix, Jean-Baptiste Fini
Published in: Frontiers in Endocrinology, 10, 2019, ISSN 1664-2392
Publisher: Frontiers
DOI: 10.3389/fendo.2019.00743

The ENDpoiNTs Project: Novel Testing Strategies for Endocrine Disruptors Linked to Developmental Neurotoxicity

Author(s): Diana Lupu, Patrik Andersson, Carl-Gustaf Bornehag, Barbara Demeneix, Ellen Fritsche, Chris Gennings, Walter Lichtensteiger, Marcel Leist, Pim E. G. Leonards, Anne-Louise Ponsonby, Martin Scholze, Giuseppe Testa, Jesus A. F. Tresguerres, Remco H. S. Westerink, Bernard Zalc, Joëlle Rüegg
Published in: International Journal of Molecular Sciences, 21/11, 2020, Page(s) 3978, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21113978

Evidence for Prenatal Exposure to Thyroid Disruptors and Adverse Effects on Brain Development

Author(s): Barbara A. Demeneix
Published in: European Thyroid Journal, 8/6, 2019, Page(s) 283-292, ISSN 2235-0640
Publisher: S. Karger AG
DOI: 10.1159/000504668

TBBPA targets converging key events of human oligodendrocyte development resulting in two novel AOPs

Author(s): Jördis Klose, Julia Tigges, Stefan Masjosthusmann, Katharina Schmuck, Farina Bendt, Ulrike Hübenthal, Patrick Petzsch, Karl Köhrer, Katharina Koch, Ellen Fritsche
Published in: ALTEX, 2021, ISSN 1868-8551
Publisher: Springer spectrum
DOI: 10.14573/altex.2007201

Handling deviating control values in concentration-response curves.

Author(s): Franziska Kappenberg; Tim Brecklinghaus; Wiebke Albrecht; Jonathan Blum; Carola van der Wurp; Marcel Leist; Jan G. Hengstler; Jörg Rahnenführer
Published in: Archives of Toxicology, 2, 2020, ISSN 1432-0738
Publisher: Springer Verlag
DOI: 10.1007/s00204-020-02913-0

Culture of Rat Primary Cortical Cells for Microelectrode Array (MEA) Recordings to Screen for Acute and Developmental Neurotoxicity

Author(s): Lora-Sophie Gerber, Lennart V J van Melis, Regina G D M van Kleef, Aart de Groot, Remco H S Westerink
Published in: Curr Protoc . 2021 Jun;1(6):e158, 2021, ISSN 2691-1299
Publisher: Wiley
DOI: 10.1002/cpz1.158

The sociability spectrum: evidence from reciprocal genetic copy number variations.

Author(s): Alejandro López-Tobón; Alejandro López-Tobón; Sebastiano Trattaro; Sebastiano Trattaro; Giuseppe Testa; Giuseppe Testa
Published in: Molecular Autism, Vol 11, Iss 1, Pp 1-13 (2020), 2, 2020, ISSN 2040-2392
Publisher: BioMed Central
DOI: 10.1186/s13229-020-00347-0

Kinetic modeling of stem cell transcriptome dynamics to identify regulatory modules of normal and disturbed neuroectodermal differentiation.

Author(s): Johannes Meisig; Johannes Meisig; Nadine Dreser; Marion Kapitza; Margit Henry; Tamara Rotshteyn; Jörg Rahnenführer; Jan G. Hengstler; Agapios Sachinidis; Tanja Waldmann; Marcel Leist; Nils Blüthgen; Nils Blüthgen
Published in: Nucleic Acids Research, 1, 2020, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa1089

Human Cortical Organoids Expose a Differential Function of GSK3 on Cortical Neurogenesis

Author(s): Alejandro López-Tobón, Carlo Emanuele Villa, Cristina Cheroni, Sebastiano Trattaro, Nicolò Caporale, Paola Conforti, Raffaele Iennaco, Maria Lachgar, Marco Tullio Rigoli, Berta Marcó de la Cruz, Pietro Lo Riso, Erika Tenderini, Flavia Troglio, Marco De Simone, Isabel Liste-Noya, Giuseppe Macino, Massimiliano Pagani, Elena Cattaneo, Giuseppe Testa
Published in: Stem Cell Reports, 13/5, 2019, Page(s) 847-861, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2019.09.005

Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies

Author(s): Markus Brüll
Published in: ALTEX, 37(3), 2020, Page(s) 409-428, ISSN 1868-596X
Publisher: Springer-Verlag GmbH
DOI: 10.14573/altex.1911111

Myelin replacement triggered by single-cell demyelination in mouse cortex.

Author(s): Nicolas Snaidero; Martina Schifferer; Aleksandra Mezydlo; Bernard Zalc; Martin Kerschensteiner; Thomas Misgeld; Thomas Misgeld
Published in: Nature Communications, 8, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-18632-0

SUIKER: Quantification of antigens in cell organelles, neurites and cellular sub-structures by imaging

Author(s): Christiaan Karreman
Published in: ALTEX, 36(3), 2019, Page(s) 518-520, ISSN 1868-596X
Publisher: Springer - Verlag GMB
DOI: 10.14573/altex.1906251