Developing an Artificial Intestine for the sustainable farming of healthy fish

Información del proyecto

Fish-AI

Identificador del acuerdo de subvención: 828835

DOI

10.3030/828835

Proyecto cerrado

Fecha de la firma de la CE 18 Diciembre 2018

Fecha de inicio 1 Abril 2019

Fecha de finalización 31 Diciembre 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 998 650,00

Aportación de la UE

€ 2 998 650,00

Coordinado por

UNIVERSITA DEGLI STUDI DI MILANO
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Websites, patent fillings, videos etc. (10)

Description of the dissemination events hold in 2023

Each year the project beneficiaries will be present at different dissemination events where they will interact with the general public explaining the progree achieved by the project. The beneficiaries will provide a description of the events that took place during the previous year.

Lab open days for school children and young adults 2

Each beneficiary will organize a lab open day either directly or through the initiatives of their own Institutions.

Report on the encounters with stakeholders during public meetings

During the last 12 months the beneficiaries will organise enounters with the stake holders both from the feed industry and the aquaculture industry sides. The aim will be to illustrate the advantages brought by the use of the fully functional and validated FISH-AI platform

Participation to the Annual European Researchers night 2

Each year the project beneficiaries will be present at The European Researchers' Night closest to its location (each September). However since the participation to this specific events is limited by a selection process, the beneficiaries will take part to any other suitable public engament events according to local availability

Lab open days for school children and young adults 1

Each beneficiary will organize a lab open day either directly or through the initiatives of their own Institutions.

Production of display and demonstration material 1

Dissemination material will be produced and shared among partners

Description of the dissemination events hold in 2024

Each year the project beneficiaries will be present at different dissemination events where they will interact with the general public explaining the progress achieved by the project. The beneficiaries will provide a description of the events that took place during the previous year.

Participation to the Annual European Researchers night 1

Each year the project beneficiaries will be present at The European Researchers' Night closest to its location (each September).

Web-site, Facebook and Tweeter accounts

From the begining of the project and along its entire duration the partners will ensure a constant flow of information suitable for the general public in order to facilitate the interaction with the common citizens

Production of display and demonstration material 2

Dissemination material will be produced and shared among partners

Documents, reports (6)

Publication in Open Access Journals

Presumably around month 18 the consortium will have generated enough new data to be assembled in one or more scientific publications in open access peer reviewed journalsThis WP will continue until month 48 and beyond untill all results will be published

Report on the press releases through the social media for milestones and special achievements in 2023

Report on the press releases for milestone and special achievements of all Participants through the project social media

Proceedings of partecipation to and workshop organization in scientific conferences

Similarly to D33 all members will communicate theyr results in high level scientific meeting and whenever possible will organize specific workshop within such meetings with the aim to disseminate the results and stimulate a fruitful scientific discussion with Colleagues that do not belong to the project

Report on the press releases through the social media for milestones and special achievements in 2024

Report on the press releases for milestone and special achievements of all Participants through the project social media

Dissemination and Exploitation Plan

A Dissemination and Exploitation Plan will be prepared at the end of the first Reporting Period (RP1). It will be updated for the subsequent Reporting Periods.

Press release for milestone and special achievements 1

Preparation of Press release for milestone and special achievements on behalf of all Participants

Open Research Data Pilot (1)

Final Fish-Ai assessment report

Identification of the most relevant parameters obtained from the Fish-AI platform and their accuracy for the prediction of the different nutritional and health values of the different tested diets and recommendation on use of the novel raw materials tested in aquafeeds. Determination of the TRL reached by the platform at the end of the project and assesment of its potential commercial applications.

Publicaciones

Peer reviewed articles (18)

Evaluation of rainbow trout (Oncorhynchus mykiss) organotypic intestinal platforms: cellular responses after long-term exposure to in vitro digested feed

Autores: Verdile, Nicole Camin, Federica Chacon, Marcelo A. Pasquariello, Rolando Pavlovic, Radmila Peggs, David Fontanillas, Ramon Tandler, Amos Kortner, Trond M. Bitan, Amir Brevini, Tiziana A. L. Gandolfi, Fulvio
Publicado en: Frontiers in Marine Science, Edición Volume 10, 2023, Página(s) 1239682, ISSN 2296-7745
Editor: Frontiers Media S.A.
DOI: 10.3389/fmars.2023.1239682

Telocytes: Active players in the rainbow trout (oncorhynchus mykiss) intestinal stem-cell niche

Autores: Verdile, Nicole Pasquariello, Rolando Cardinaletti, Gloriana Tibaldi, Emilio Brevini, Tiziana A.L. Gandolfi, Fulvio
Publicado en: Animals, Edición 74, 12 (1), 2022, ISSN 2076-2615
Editor: MDPI
DOI: 10.3390/ani12010074

Organ-on-Chip Approaches for Intestinal 3D In Vitro Modeling

Autores: Joana Pimenta, Ricardo Ribeiro, Raquel Almeida, Pedro F. Costa, Marta A. da Silva, Bruno Pereira
Publicado en: Cellular and Molecular Gastroenterology and Hepatology, Edición 13, 2022, Página(s) 351-367, ISSN 2352-345X
Editor: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2021.08.015

Bile components affect the functions and transcriptome of the rainbow trout intestinal epithelial cell line RTgutGC

Autores: Guro Løkka, Anusha K.S. Dhanasiri, Åshild Krogdahl, Trond M. Kortner
Publicado en: Fish & Shellfish Immunology, Edición 131, 2024, Página(s) 1144-1156, ISSN 1050-4648
Editor: Academic Press
DOI: 10.1016/j.fsi.2022.10.049

Development of a Rainbow Trout (Oncorhynchus mykiss) Intestinal In Vitro Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet

Autores: Pasquariello, Rolando Pavlovic, Radmila Chacon, Marcelo A. Camin, Federica Verdile, Nicole Løkka, Guro Panseri, Sara Faustini, Massimo Tandler, Amos Peggs, David Kortner, Trond M. Bitan, Amir Brevini, Tiziana .A.L. Gandolfi, Fulvio
Publicado en: Animals, Edición volume 13 Edición 14, 2023, Página(s) 2278-2298, ISSN 2076-2615
Editor: MDPI
DOI: 10.3390/ani13142278

A fish intestinal in vitro model for investigation of lipid metabolism and steatosis

Autores: Daphne Siciliani, Bente Ruyter, Guro Løkka, Kirsti Elisabeth Præsteng, Matteo Minghetti, Trond M. Kortner
Publicado en: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Edición 1870, 2024, Página(s) 159573, ISSN 1388-1981
Editor: Elsevier BV
DOI: 10.1016/j.bbalip.2024.159573

Digital light processing of photo-crosslinkable gelatin to create biomimetic 3D constructs serving small intestinal tissue regeneration

Autores: Laure Maes, Anna Szabó, Jens Van Haevermaete, Indi Geurs, Koen Dewettinck, Roosmarijn E. Vandenbroucke, Sandra Van Vlierberghe, Debby Laukens
Publicado en: Biomaterials Advances, Edición 171, 2025, Página(s) 214232, ISSN 2772-9508
Editor: Elsevier BV
DOI: 10.1016/j.bioadv.2025.214232

Light-Based 3D Printing of Gelatin-Based Biomaterial Inks to Create a Physiologically Relevant In Vitro Fish Intestinal Model

Autores: Anna Szabó, Rolando Pasquariello, Pedro F. Costa, Radmila Pavlovic, Indi Geurs, Koen Dewettinck, Chris Vervaet, Tiziana A. L. Brevini, Fulvio Gandolfi, Sandra Van Vlierberghe
Publicado en: Macromolecular Bioscience, Edición 2023, 2300016, 2023, Página(s) 1-11, ISSN 1616-5195
Editor: Wiley-VCH GmbH,
DOI: 10.1002/mabi.202300016

The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells

Autores: Nicole Verdile, Rolando Pasquariello, Tiziana A. L. Brevini, Fulvio Gandolfi
Publicado en: International Journal of Molecular Sciences, Edición 21/23, 2020, Página(s) 9192, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21239192

Ectopic stem cell niches sustain rainbow trout (Oncorhynchus mykiss) intestine absorptive capacity when challenged with a plant protein-rich diet

Autores: Nicole Verdile, Gloriana Cardinaletti, Filippo Faccenda, Tiziana A.L. Brevini, Fulvio Gandolfi, Emilio Tibaldi
Publicado en: Aquaculture, Edición 564, 2023, Página(s) 739031, ISSN 0044-8486
Editor: Elsevier BV
DOI: 10.1016/j.aquaculture.2022.739031

Distinct Organotypic Platforms Modulate Rainbow Trout (Oncorhynchus mykiss) Intestinal Cell Differentiation In Vitro

Autores: Verdile, Nicole Camin, Federica Pavlovic, Radmila Pasquariello, Rolando Stuknytė, Milda De Noni, Ivano Brevini, Tiziana A.L. Gandolfi, Fulvio
Publicado en: Cells, Edición Volume 12 Edición 14, 2023, Página(s) 1843, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells12141843

Cell-Interactive Gelatin-Based ¹⁹F MRI Tracers: An <i>In Vitro</i> Proof-of-Concept Study

Autores: Kristyna Kolouchova, Ondrej Groborz, Vit Herynek, Oleg V. Petrov, Jan Lang, David Dunlop, Laurens Parmentier, Anna Szabó, David Schaubroeck, Peter Adriaensens, Sandra Van Vlierberghe
Publicado en: Chemistry of Materials, Edición 36, 2024, Página(s) 183-196, ISSN 0897-4756
Editor: American Chemical Society
DOI: 10.1021/acs.chemmater.3c01574

Effect of Porosity on the Colonization of Digital Light-Processed 3D Hydrogel Constructs toward the Development of a Functional Intestinal Model

Autores: Anna Szabó, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Laure Maes, Debby Laukens, Sandra Van Vlierberghe
Publicado en: Biomacromolecules, Edición 25, 2024, Página(s) 2863-2874, ISSN 1525-7797
Editor: American Chemical Society
DOI: 10.1021/acs.biomac.4c00019

A Detailed Study of Rainbow Trout (Onchorhynchus mykiss) Intestine Revealed That Digestive and Absorptive Functions Are Not Linearly Distributed along Its Length

Autores: Nicole Verdile, Rolando Pasquariello, Marco Scolari, Giulia Scirè, Tiziana A. L. Brevini, Fulvio Gandolfi
Publicado en: Animals, Edición 10/4, 2020, Página(s) 745, ISSN 2076-2615
Editor: MDPI
DOI: 10.3390/ani10040745

Use of a rainbow trout (Oncorhynchus mykiss) intestinal in vitro platform to evaluate different diets

Autores: F. Camin, N. Verdile, M.A. Chacon, G. Løkka, D. Peggs, A. Tandler, A. Bitan, T.M. Kortner, R. Fontanillas, T.A.L. Brevini, F. Gandolfi
Publicado en: Frontiers in Marine Science, Edición 12, 2025, Página(s) 01-10, ISSN 2296-7745
Editor: Frontiers Media SA
DOI: 10.3389/fmars.2025.1576618

Increasing hydrogel complexity from 2D towards 3D towards intestinal tissue engineering

Autores: Anna Szabó, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Sandra Van Vlierberghe
Publicado en: Giant, Edición 16, 2023, Página(s) 100198, ISSN 2666-5425
Editor: Elsevier Ltd
DOI: 10.1016/j.giant.2023.100198

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

Autores: Rolando Pasquariello, Nicole Verdile, Radmila Pavlovic, Sara Panseri, Kristin Schirmer, Tiziana A. L. Brevini, Fulvio Gandolfi
Publicado en: Cells, Edición 10/6, 2021, Página(s) 1555, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells10061555

New Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout (Oncorhynchus mykiss) Intestinal Platform

Autores: Nicole Verdile, Nico Cattaneo, Federica Camin, Matteo Zarantoniello, Federico Conti, Gloriana Cardinaletti, Tiziana A. L. Brevini, Ike Olivotto, Fulvio Gandolfi
Publicado en: Cells, Edición 14, 2025, Página(s) 44, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells14010044

Book chapters (2)

Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa

Autores: Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A. L. Brevini, Sandra Van Vlierberghe, Fulvio Gandolfi
Publicado en: Methods in Molecular Biology, Next Generation Culture Platforms for Reliable In Vitro Models, 2021, Página(s) 263-278
Editor: Springer US
DOI: 10.1007/978-1-0716-1246-0_19

Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa

Autores: Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A. L. Brevini, Sandra Van Vlierberghe, Fulvio Gandolfi
Publicado en: Next Generation Culture Platforms for Reliable In Vitro Models - Methods and Protocols, Edición 2273, 2021, Página(s) 263-278, ISBN 978-1-0716-1245-3
Editor: Springer US
DOI: 10.1007/978-1-0716-1246-0_19#doi

Derechos de propiedad intelectual

Registered design (3)

N.A.

Número de solicitud/publicación: PT Design Protection 6787
Fecha: 2022-11-02
Solicitante(s): BIOFABICS LDA

DISPOSITIVO DE PERFUSÃO COM MECANISMO DE PRESSÃO AJUSTÁVEL BASEADO EM TORÇÃO PARA MANTER AS MEMBRANAS FIRMEMENTE NO LUGAR, CAPAZ DE DEFINIR DUAS CÂMARAS DE PERFUSÃO DE FLUIDO INDEPENDENTES E ADJACENTES, BEM COMO DOIS RESERVATÓRIOS DE FLUIDO INDEPENDENTES QUANDO COLOCADOS NUM RECIPIENTE PLANO.

Número de solicitud/publicación: PT Design Protection 6769
Fecha: 2022-09-30
Solicitante(s): BIOFABICS LDA

TAMPA UNIVERSAL PARA COBERTURA DE PLACAS DE POÇOS ATRAVÉS DA QUAL É POSSÍVEL INJETAR, EXTRAIR E PERFUNDIR FLUIDOS UTILIZANDO AGULHAS, CÂNULAS, TUBOS OU OUTROS UTENSÍLIOS DE MANIPULAÇÃO DE FLUIDOS.

Número de solicitud/publicación: PT Design Protection 7149
Fecha: 2024-06-16
Solicitante(s): BIOFABICS LDA