Developing an Artificial Intestine for the sustainable farming of healthy fish

Informations projet

Fish-AI

N° de convention de subvention: 828835

DOI

10.3030/828835

Projet clôturé

Date de signature de la CE 18 Decembre 2018

Date de début 1 Avril 2019

Date de fin 31 Decembre 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 998 650,00

Contribution de l’UE

€ 2 998 650,00

Coordonné par

UNIVERSITA DEGLI STUDI DI MILANO
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Websites, patent fillings, videos etc. (10)

Description of the dissemination events hold in 2023

Each year the project beneficiaries will be present at different dissemination events where they will interact with the general public explaining the progree achieved by the project. The beneficiaries will provide a description of the events that took place during the previous year.

Lab open days for school children and young adults 2

Each beneficiary will organize a lab open day either directly or through the initiatives of their own Institutions.

Report on the encounters with stakeholders during public meetings

During the last 12 months the beneficiaries will organise enounters with the stake holders both from the feed industry and the aquaculture industry sides. The aim will be to illustrate the advantages brought by the use of the fully functional and validated FISH-AI platform

Participation to the Annual European Researchers night 2

Each year the project beneficiaries will be present at The European Researchers' Night closest to its location (each September). However since the participation to this specific events is limited by a selection process, the beneficiaries will take part to any other suitable public engament events according to local availability

Lab open days for school children and young adults 1

Each beneficiary will organize a lab open day either directly or through the initiatives of their own Institutions.

Production of display and demonstration material 1

Dissemination material will be produced and shared among partners

Description of the dissemination events hold in 2024

Each year the project beneficiaries will be present at different dissemination events where they will interact with the general public explaining the progress achieved by the project. The beneficiaries will provide a description of the events that took place during the previous year.

Participation to the Annual European Researchers night 1

Each year the project beneficiaries will be present at The European Researchers' Night closest to its location (each September).

Web-site, Facebook and Tweeter accounts

From the begining of the project and along its entire duration the partners will ensure a constant flow of information suitable for the general public in order to facilitate the interaction with the common citizens

Production of display and demonstration material 2

Dissemination material will be produced and shared among partners

Documents, reports (6)

Publication in Open Access Journals

Presumably around month 18 the consortium will have generated enough new data to be assembled in one or more scientific publications in open access peer reviewed journalsThis WP will continue until month 48 and beyond untill all results will be published

Report on the press releases through the social media for milestones and special achievements in 2023

Report on the press releases for milestone and special achievements of all Participants through the project social media

Proceedings of partecipation to and workshop organization in scientific conferences

Similarly to D33 all members will communicate theyr results in high level scientific meeting and whenever possible will organize specific workshop within such meetings with the aim to disseminate the results and stimulate a fruitful scientific discussion with Colleagues that do not belong to the project

Report on the press releases through the social media for milestones and special achievements in 2024

Report on the press releases for milestone and special achievements of all Participants through the project social media

Dissemination and Exploitation Plan

A Dissemination and Exploitation Plan will be prepared at the end of the first Reporting Period (RP1). It will be updated for the subsequent Reporting Periods.

Press release for milestone and special achievements 1

Preparation of Press release for milestone and special achievements on behalf of all Participants

Open Research Data Pilot (1)

Final Fish-Ai assessment report

Identification of the most relevant parameters obtained from the Fish-AI platform and their accuracy for the prediction of the different nutritional and health values of the different tested diets and recommendation on use of the novel raw materials tested in aquafeeds. Determination of the TRL reached by the platform at the end of the project and assesment of its potential commercial applications.

Publications

Peer reviewed articles (18)

Evaluation of rainbow trout (Oncorhynchus mykiss) organotypic intestinal platforms: cellular responses after long-term exposure to in vitro digested feed

Auteurs: Verdile, Nicole Camin, Federica Chacon, Marcelo A. Pasquariello, Rolando Pavlovic, Radmila Peggs, David Fontanillas, Ramon Tandler, Amos Kortner, Trond M. Bitan, Amir Brevini, Tiziana A. L. Gandolfi, Fulvio
Publié dans: Frontiers in Marine Science, Numéro Volume 10, 2023, Page(s) 1239682, ISSN 2296-7745
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fmars.2023.1239682

Telocytes: Active players in the rainbow trout (oncorhynchus mykiss) intestinal stem-cell niche

Auteurs: Verdile, Nicole Pasquariello, Rolando Cardinaletti, Gloriana Tibaldi, Emilio Brevini, Tiziana A.L. Gandolfi, Fulvio
Publié dans: Animals, Numéro 74, 12 (1), 2022, ISSN 2076-2615
Éditeur: MDPI
DOI: 10.3390/ani12010074

Organ-on-Chip Approaches for Intestinal 3D In Vitro Modeling

Auteurs: Joana Pimenta, Ricardo Ribeiro, Raquel Almeida, Pedro F. Costa, Marta A. da Silva, Bruno Pereira
Publié dans: Cellular and Molecular Gastroenterology and Hepatology, Numéro 13, 2022, Page(s) 351-367, ISSN 2352-345X
Éditeur: Elsevier Inc.
DOI: 10.1016/j.jcmgh.2021.08.015

Bile components affect the functions and transcriptome of the rainbow trout intestinal epithelial cell line RTgutGC

Auteurs: Guro Løkka, Anusha K.S. Dhanasiri, Åshild Krogdahl, Trond M. Kortner
Publié dans: Fish & Shellfish Immunology, Numéro 131, 2024, Page(s) 1144-1156, ISSN 1050-4648
Éditeur: Academic Press
DOI: 10.1016/j.fsi.2022.10.049

Development of a Rainbow Trout (Oncorhynchus mykiss) Intestinal In Vitro Platform for Profiling Amino Acid Digestion and Absorption of a Complete Diet

Auteurs: Pasquariello, Rolando Pavlovic, Radmila Chacon, Marcelo A. Camin, Federica Verdile, Nicole Løkka, Guro Panseri, Sara Faustini, Massimo Tandler, Amos Peggs, David Kortner, Trond M. Bitan, Amir Brevini, Tiziana .A.L. Gandolfi, Fulvio
Publié dans: Animals, Numéro volume 13 Numéro 14, 2023, Page(s) 2278-2298, ISSN 2076-2615
Éditeur: MDPI
DOI: 10.3390/ani13142278

A fish intestinal in vitro model for investigation of lipid metabolism and steatosis

Auteurs: Daphne Siciliani, Bente Ruyter, Guro Løkka, Kirsti Elisabeth Præsteng, Matteo Minghetti, Trond M. Kortner
Publié dans: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Numéro 1870, 2024, Page(s) 159573, ISSN 1388-1981
Éditeur: Elsevier BV
DOI: 10.1016/j.bbalip.2024.159573

Digital light processing of photo-crosslinkable gelatin to create biomimetic 3D constructs serving small intestinal tissue regeneration

Auteurs: Laure Maes, Anna Szabó, Jens Van Haevermaete, Indi Geurs, Koen Dewettinck, Roosmarijn E. Vandenbroucke, Sandra Van Vlierberghe, Debby Laukens
Publié dans: Biomaterials Advances, Numéro 171, 2025, Page(s) 214232, ISSN 2772-9508
Éditeur: Elsevier BV
DOI: 10.1016/j.bioadv.2025.214232

Light-Based 3D Printing of Gelatin-Based Biomaterial Inks to Create a Physiologically Relevant In Vitro Fish Intestinal Model

Auteurs: Anna Szabó, Rolando Pasquariello, Pedro F. Costa, Radmila Pavlovic, Indi Geurs, Koen Dewettinck, Chris Vervaet, Tiziana A. L. Brevini, Fulvio Gandolfi, Sandra Van Vlierberghe
Publié dans: Macromolecular Bioscience, Numéro 2023, 2300016, 2023, Page(s) 1-11, ISSN 1616-5195
Éditeur: Wiley-VCH GmbH,
DOI: 10.1002/mabi.202300016

The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells

Auteurs: Nicole Verdile, Rolando Pasquariello, Tiziana A. L. Brevini, Fulvio Gandolfi
Publié dans: International Journal of Molecular Sciences, Numéro 21/23, 2020, Page(s) 9192, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21239192

Ectopic stem cell niches sustain rainbow trout (Oncorhynchus mykiss) intestine absorptive capacity when challenged with a plant protein-rich diet

Auteurs: Nicole Verdile, Gloriana Cardinaletti, Filippo Faccenda, Tiziana A.L. Brevini, Fulvio Gandolfi, Emilio Tibaldi
Publié dans: Aquaculture, Numéro 564, 2023, Page(s) 739031, ISSN 0044-8486
Éditeur: Elsevier BV
DOI: 10.1016/j.aquaculture.2022.739031

Distinct Organotypic Platforms Modulate Rainbow Trout (Oncorhynchus mykiss) Intestinal Cell Differentiation In Vitro

Auteurs: Verdile, Nicole Camin, Federica Pavlovic, Radmila Pasquariello, Rolando Stuknytė, Milda De Noni, Ivano Brevini, Tiziana A.L. Gandolfi, Fulvio
Publié dans: Cells, Numéro Volume 12 Numéro 14, 2023, Page(s) 1843, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells12141843

Cell-Interactive Gelatin-Based ¹⁹F MRI Tracers: An <i>In Vitro</i> Proof-of-Concept Study

Auteurs: Kristyna Kolouchova, Ondrej Groborz, Vit Herynek, Oleg V. Petrov, Jan Lang, David Dunlop, Laurens Parmentier, Anna Szabó, David Schaubroeck, Peter Adriaensens, Sandra Van Vlierberghe
Publié dans: Chemistry of Materials, Numéro 36, 2024, Page(s) 183-196, ISSN 0897-4756
Éditeur: American Chemical Society
DOI: 10.1021/acs.chemmater.3c01574

Effect of Porosity on the Colonization of Digital Light-Processed 3D Hydrogel Constructs toward the Development of a Functional Intestinal Model

Auteurs: Anna Szabó, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Laure Maes, Debby Laukens, Sandra Van Vlierberghe
Publié dans: Biomacromolecules, Numéro 25, 2024, Page(s) 2863-2874, ISSN 1525-7797
Éditeur: American Chemical Society
DOI: 10.1021/acs.biomac.4c00019

A Detailed Study of Rainbow Trout (Onchorhynchus mykiss) Intestine Revealed That Digestive and Absorptive Functions Are Not Linearly Distributed along Its Length

Auteurs: Nicole Verdile, Rolando Pasquariello, Marco Scolari, Giulia Scirè, Tiziana A. L. Brevini, Fulvio Gandolfi
Publié dans: Animals, Numéro 10/4, 2020, Page(s) 745, ISSN 2076-2615
Éditeur: MDPI
DOI: 10.3390/ani10040745

Use of a rainbow trout (Oncorhynchus mykiss) intestinal in vitro platform to evaluate different diets

Auteurs: F. Camin, N. Verdile, M.A. Chacon, G. Løkka, D. Peggs, A. Tandler, A. Bitan, T.M. Kortner, R. Fontanillas, T.A.L. Brevini, F. Gandolfi
Publié dans: Frontiers in Marine Science, Numéro 12, 2025, Page(s) 01-10, ISSN 2296-7745
Éditeur: Frontiers Media SA
DOI: 10.3389/fmars.2025.1576618

Increasing hydrogel complexity from 2D towards 3D towards intestinal tissue engineering

Auteurs: Anna Szabó, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Sandra Van Vlierberghe
Publié dans: Giant, Numéro 16, 2023, Page(s) 100198, ISSN 2666-5425
Éditeur: Elsevier Ltd
DOI: 10.1016/j.giant.2023.100198

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (Oncorhynchus mykiss) Retain Several Properties Observed In Vivo

Auteurs: Rolando Pasquariello, Nicole Verdile, Radmila Pavlovic, Sara Panseri, Kristin Schirmer, Tiziana A. L. Brevini, Fulvio Gandolfi
Publié dans: Cells, Numéro 10/6, 2021, Page(s) 1555, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells10061555

New Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout (Oncorhynchus mykiss) Intestinal Platform

Auteurs: Nicole Verdile, Nico Cattaneo, Federica Camin, Matteo Zarantoniello, Federico Conti, Gloriana Cardinaletti, Tiziana A. L. Brevini, Ike Olivotto, Fulvio Gandolfi
Publié dans: Cells, Numéro 14, 2025, Page(s) 44, ISSN 2073-4409
Éditeur: MDPI
DOI: 10.3390/cells14010044

Book chapters (2)

Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa

Auteurs: Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A. L. Brevini, Sandra Van Vlierberghe, Fulvio Gandolfi
Publié dans: Methods in Molecular Biology, Next Generation Culture Platforms for Reliable In Vitro Models, 2021, Page(s) 263-278
Éditeur: Springer US
DOI: 10.1007/978-1-0716-1246-0_19

Preparation of Biological Scaffolds and Primary Intestinal Epithelial Cells to Efficiently 3D Model the Fish Intestinal Mucosa

Auteurs: Nicole Verdile, Anna Szabó, Rolando Pasquariello, Tiziana A. L. Brevini, Sandra Van Vlierberghe, Fulvio Gandolfi
Publié dans: Next Generation Culture Platforms for Reliable In Vitro Models - Methods and Protocols, Numéro 2273, 2021, Page(s) 263-278, ISBN 978-1-0716-1245-3
Éditeur: Springer US
DOI: 10.1007/978-1-0716-1246-0_19#doi

Droits de propriété intellectuelle

Registered design (3)

N.A.

Numéro de demande/publication: PT Design Protection 6787
Date: 2022-11-02
Demandeur(s): BIOFABICS LDA

DISPOSITIVO DE PERFUSÃO COM MECANISMO DE PRESSÃO AJUSTÁVEL BASEADO EM TORÇÃO PARA MANTER AS MEMBRANAS FIRMEMENTE NO LUGAR, CAPAZ DE DEFINIR DUAS CÂMARAS DE PERFUSÃO DE FLUIDO INDEPENDENTES E ADJACENTES, BEM COMO DOIS RESERVATÓRIOS DE FLUIDO INDEPENDENTES QUANDO COLOCADOS NUM RECIPIENTE PLANO.

Numéro de demande/publication: PT Design Protection 6769
Date: 2022-09-30
Demandeur(s): BIOFABICS LDA

TAMPA UNIVERSAL PARA COBERTURA DE PLACAS DE POÇOS ATRAVÉS DA QUAL É POSSÍVEL INJETAR, EXTRAIR E PERFUNDIR FLUIDOS UTILIZANDO AGULHAS, CÂNULAS, TUBOS OU OUTROS UTENSÍLIOS DE MANIPULAÇÃO DE FLUIDOS.

Numéro de demande/publication: PT Design Protection 7149
Date: 2024-06-16
Demandeur(s): BIOFABICS LDA