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Next generation precision antibody profiling - from science fiction to reality

Objective

One of the major challenges of modern medicine is to understand how the human organism defends itself against invasions and diseases. The biggest mystery is the human immune system, and understanding this ultimately requires knowledge of the sequence repertoire of human antibodies and their respective antigens. The purpose of the TopSpec project is to be the first in the world to solve this challenge, opening up opportunities in medical research and drug development that are today only dreamt about. We will create a breakthrough technology that will revolutionize academic, clinical and industrial proteomics and dramatically advance the development of new generation antibody- and protein-based therapeutics. Antibodies represent the most sophisticated line of natural defense against disease. Knowing exactly which antibodies are produced in response to a particular disease enables us not only to better understand the cause of the disease but also to provide new-generation cures in the form of personalized therapeutic antibodies. The limiting factor for this to truly be achieved is to find a way to analyze and sequence large molecules in the gas phase, and this represents a formidable challenge. The TopSpec project will develop ground-breaking TOP-down tandem mass SPECtrometry (MS/MS) approaches based on novel radical gas-phase ion-electron and ion-atom reactions, and implement them on a unique, hyphenated, ultrahigh-resolution MS platform. Another “killer innovation” is the ability to greatly simplify MS/MS spectra of large molecules by adding another dimension of separation – collisional cross-sections of fragment ions using two parallel approaches. TopSpec will be the first project to implement de-convolution of massively overlapping isotopic clusters, solving one of the greatest challenges in top-down MS of large molecules.

Call for proposal

H2020-FETOPEN-2018-2019-2020-01
See other projects for this call

Coordinator

KAROLINSKA INSTITUTET
Address
Nobels Vag 5
17177 Stockholm
Sweden
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 989 500

Participants (7)

FASMATECH EPISTIMONIKI KAI TECHNOLOGIKI ANONYMI ETAIREIA
Greece
EU contribution
€ 860 500
Address
Patriarchou Grigoriou E Kai Neapoleos, Lefkippos Technology Park, Ncsr Demokritos, Building 27
153 41 Agia Paraskevi
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
THERMO FISHER SCIENTIFIC (BREMEN) GMBH
Germany
EU contribution
€ 695 500
Address
Hanna Kunath Strasse 11
28199 Bremen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
SPECTROSWISS SARL
Switzerland
EU contribution
€ 430 500
Address
Epfl Innovation Park Building I
1015 Lausanne
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOMOTIF AB
Sweden
EU contribution
€ 325 000
Address
Wanning Kometvagen 17
18348 Stockholm
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
THE NOTTINGHAM TRENT UNIVERSITY
United Kingdom
EU contribution
€ 306 500
Address
50 Shakespeare Street
NG1 4FQ Nottingham
Activity type
Higher or Secondary Education Establishments
INSTITUT PASTEUR
France
EU contribution
€ 278 500
Address
Rue Du Docteur Roux 25-28
75724 Paris Cedex 15
Activity type
Research Organisations
SPECTROMETRY VISION BV
Netherlands
EU contribution
€ 105 500
Address
Televisieweg 40
1322 AM Almere
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)