A regenerative medicine approach in diabetes.

Project Information

EYELETS

Grant agreement ID: 834860

DOI

10.3030/834860

Project closed

EC signature date 2 May 2019

Start date 1 January 2020

End date 30 June 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 500 000,00

EU contribution

€ 2 500 000,00

Coordinated by

KAROLINSKA INSTITUTET
Sweden

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (43)

Challenges in stem cell-derived islet replacement therapy can be overcome

Author(s): MH Abdulreda, P-O Berggren
Published in: Cell Transplantation, Issue 30, 2021, Page(s) 1-5, ISSN 1555-3892
Publisher: SAGE Publications
DOI: 10.1177/09636897211045320

The anterior chamber of the eye technology and its anatomical, optical, and immunological bases

Author(s): Shao-Nian Yang, Yue Shi, Per-Olof Berggren
Published in: Physiological Reviews, Issue 104, 2024, Page(s) 881-929, ISSN 0031-9333
Publisher: American Physiological Society
DOI: 10.1152/physrev.00024.2023

Treatment of the metabolic syndrome by siRNA targeting apolipoprotein CIII

Author(s): Patricia Recio‐López, Ismael Valladolid‐Acebes, Philipp Hadwiger, Markus Hossbach, Monika Krampert, Carla Prata, Per‐Olof Berggren, Lisa Juntti‐Berggren
Published in: BioFactors, Issue 49, 2023, Page(s) 153-172, ISSN 0951-6433
Publisher: International Union of Biochemistry by IRL Press
DOI: 10.1002/biof.1885

Target-Mediated EV-SNA Clustering as an Amplifier for <i>In Situ</i> miRNA Imaging in Living Cells

Author(s): Zhenghao Wang, Xinyue Zhu, Ye Zhou, Tianci Zhang, Yue Shi, Xiaofeng Zheng, Shao-Nian Yang, Wei Li, Per-Olof Berggren
Published in: Nano Letters, Issue 25, 2025, Page(s) 11860-11869, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.5c02256

Biodegradable and Antioxidant DNA Hydrogel as a Cytokine Delivery System for Diabetic Wound Healing

Author(s): Zhenghao Wang, Wei Li, Liping Gou, Ye Zhou, Ge Peng, Jiayi Zhang, Jiaye Liu, Ruoqing Li, Hengfan Ni, Wanli Zhang, Ting Cao, Qi Cao, Hong Su, Yuan‐Ping Han, Nanwei Tong, Xianghui Fu, Erwin Ilegems, Yanrong Lu, Per‐Olof Berggren, Xiaofeng Zheng, Chengshi Wang
Published in: Advanced Healthcare Materials, Issue 11, 2025, ISSN 2192-2640
Publisher: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202200782

Local Dexamethasone Administration Delays Allogeneic Islet Graft Rejection in the Anterior Chamber of the Eye of Non-Human Primates

Author(s): Sai Bo Bo Tun, Minni Chua, Gavin Siew Wei Tan, Ingo Leibiger, Yusuf Ali, Veluchamy Amutha Barathi, Per-Olof Berggren
Published in: Cell Transplantation, Issue Volume 31, 2022, Page(s) 1-14, ISSN 0963-6897
Publisher: Cognizant Communication Corp.
DOI: 10.1177/09636897221098038

In vivo Ca 2+ dynamics in single pancreatic β cells

Author(s): Stefan Jacob, Martin Köhler, Philip Tröster, Montse Visa, Concha F. García‐Prieto, Tomas Alanentalo, Tilo Moede, Barbara Leibiger, Ingo B. Leibiger, Per‐Olof Berggren
Published in: The FASEB Journal, Issue 34/1, 2020, Page(s) 945-959, ISSN 0892-6638
Publisher: Federation of American Societies for Experimental Biology
DOI: 10.1096/fj.201901302rr

Lowering apolipoprotein CIII protects against high-fat diet–induced metabolic derangements

Author(s): Ismael Valladolid-Acebes, Karin Åvall, Patricia Recio-López, Noah Moruzzi, Galyna Bryzgalova, Marie Björnholm, Anna Krook, Elena Fauste Alonso, Madelene Ericsson, Fredrik Landfors, Stefan K. Nilsson, Per-Olof Berggren, Lisa Juntti-Berggren
Published in: Science Advances, Issue 7/11, 2021, Page(s) eabc2931, ISSN 2375-2548
Publisher: AMER ASSOC ADVANCEMENT SCIENCE1200 NEW YORK AVE, NW, WASHINGTON, DC 20005
DOI: 10.1126/sciadv.abc2931

Intravital Ca 2+ imaging of pancreatic β cell function after bariatric surgery

Author(s): P Tröster, P-O Berggren
Published in: Cell Calcium, Issue 104, 2022, ISSN 0143-4160
Publisher: Churchill Livingstone
DOI: 10.1016/j.ceca.2022.102566

In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye

Author(s): Francesca Lazzeri-Barcelo, Pierpaolo Ciardo, Barbara Leibiger, Ingo B. Leibiger, Per-Olof Berggren, Noah Moruzzi
Published in: Journal of Visualized Experiments, 2024, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/66234

Transplantation and Noninvasive Longitudinal <i>In Vivo</i> Imaging of Parathyroid Cells: A Proof-of-Concept Study

Author(s): Robert Bränström, Pim P. van Krieken, Robin Fröbom, C. Christofer Juhlin, Ivan Shabo, Barbara Leibiger, Ingo B. Leibiger, Per-Olof Berggren, Craig A. Aspinwall
Published in: Cell Transplantation, Issue 33, 2025, ISSN 0963-6897
Publisher: Cognizant Communication Corp.
DOI: 10.1177/09636897241241995

XPR1 Mediates the Pancreatic beta-Cell Phosphate Flush

Author(s): Barker CJ, Tessaro FHG, Ferreira SS, Simas R, Ayala TS, Köhler M, Rajasekaran SS, Martins JO, Darè E, Berggren P-O
Published in: Diabetes, Issue 70, 2020, Page(s) 111-18, ISSN 0012-1797
Publisher: American Diabetes Association
DOI: 10.2337/db19-0633

The Eye as a Transplantation Site to Monitor Pancreatic Islet Cell Plasticity

Author(s): Erwin Ilegems, Per-Olof Berggren
Published in: Frontiers in Endocrinology, Issue 12, 2021, ISSN 1664-2392
Publisher: FRONTIERS MEDIA SAAVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE CH-1015
DOI: 10.3389/fendo.2021.652853

In Vivo CaV3 Channel Inhibition Promotes Maturation of Glucose-Dependent Ca2+ Signaling in Human iPSC-Islets

Author(s): Kaixuan Zhao, Yue Shi, Jia Yu, Lina Yu, Martin Köhler, Amber Mael, Anthony Kolton, Thomas Joyce, Jon Odorico, Per-Olof Berggren, Shao-Nian Yang
Published in: Biomedicines, Issue 11, 2025, Page(s) 807, ISSN 2227-9059
Publisher: MDPI AG
DOI: 10.3390/biomedicines11030807

Inward and outward currents of native and cloned K(ATP) channels (Kir6.2/SUR1) share single-channel kinetic properties

Author(s): Robert Bränström , Erik Berglund, Robin Fröbom, Ingo B Leibiger, Barbara Leibiger, Craig A Aspinwall, Olof Larsson, Per-Olof Berggren
Published in: Biochemistry and Biophysics Reports, Issue 30, 2022, ISSN 2405-5808
Publisher: Elsevier
DOI: 10.1016/j.bbrep.2022.101260

HIF-1α inhibitor PX-478 preserves pancreatic β cell function in diabetes

Author(s): Erwin Ilegems, Galyna Bryzgalova, Jorge Correia, Burcak Yesildag, Edurne Berra, Jorge L. Ruas, Teresa S. Pereira, Per-Olof Berggren
Published in: Science Translational Medicine, Issue 14, 2024, ISSN 1946-6234
Publisher: American Association for the Advancement of Science
DOI: 10.1126/scitranslmed.aba9112

Alpha cell regulation of beta cell function

Author(s): Tilo Moede, Ingo B. Leibiger, Per-Olof Berggren
Published in: Diabetologia, Issue 63/10, 2020, Page(s) 2064-2075, ISSN 0012-186X
Publisher: Springer Verlag
DOI: 10.1007/s00125-020-05196-3

β-Cell Neogenesis From the Pancreatic Ductal Epithelium Revealed Dynamically by Long-term Intravital Imaging

Author(s): Brandon Watts, Isabella Altilio, Óscar Alcázar, Silvia Álvarez-Cubela, Per-Olof Berggren, Midhat H. Abdulreda, Ricardo L. Pastori, Juan Domínguez-Bendala
Published in: Diabetes, 2025, ISSN 0012-1797
Publisher: American Diabetes Association
DOI: 10.2337/db25-0457

Sustained heterologous gene expression in pancreatic islet organoids using adeno-associated virus serotype 8

Author(s): Anna Voznesenskaya, Per-Olof Berggren, Erwin Ilegems
Published in: Frontiers in Bioengineering and Biotechnology, Issue 11, 2023, ISSN 2296-4185
Publisher: Frontiers Media SA
DOI: 10.3389/fbioe.2023.1147244

Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling

Author(s): Yan Xiong, M Julia Scerbo, Anett Seelig, Francesco Volta, Nils O'Brien, Andrea Dicker, Daniela Padula, Heiko Lickert, Jantje Mareike Gerdes, Per-Olof Berggren
Published in: eLife, Issue 9, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.56914

Diversity of respiratory parameters and metabolic adaptation to low oxygen tension in mesenchymal stromal cells

Author(s): Kim Olesen, Noah Moruzzi, Ivana Bulatovic, Clifford Folmes, Ryounghoon Jeon, Ulrika Felldin, Andre Terzic, Oscar E. Simonson, Katarina Le Blanc, Cecilia Österholm, Per-Olof Berggren, Tomas Schiffer, Sergey Rodin, Andreas Tilevik, Karl-Henrik Grinnemo
Published in: Metabolism Open, Issue 13, 2022, Page(s) 100167, ISSN 2589-9368
Publisher: Elsevier BV
DOI: 10.1016/j.metop.2022.100167

Tissue‐specific expression of insulin receptor isoforms in obesity/type 2 diabetes mouse models

Author(s): Noah Moruzzi, Francesca Lazzeri‐Barcelo, Ismael Valladolid‐Acebes, Tilo Moede, Meike Paschen, Barbara Leibiger, Per‐Olof Berggren, Ingo B. Leibiger
Published in: Journal of Cellular and Molecular Medicine, Issue 25/10, 2021, Page(s) 4800-4813, ISSN 1582-1838
Publisher: Wiley-Blackwell
DOI: 10.1111/jcmm.16452

XPR1 mediates the pancreatic β-cell phosphate flush

Author(s): Ada Admin, Christopher J. Barker, Fernando Henrique Galvão Tessaro, Sabrina de Souza Ferreira, Rafael Simas, Thais S. Ayala, Martin Köhler, Subu Surendran Rajasekaran, Joilson O. Martins, Elisabetta Darè, Per Olof Berggren
Published in: Diabetes, Issue Vol 33, 2020, ISSN 0963-6897
Publisher: Cognizant Communication Corp.
DOI: 10.2337/figshare.12821192.v1

Multiple Inositol Polyphosphate Phosphatase Compartmentalization Separates Inositol Phosphate Metabolism from Inositol Lipid Signaling

Author(s): Jia Yu, Barbara Leibiger, Shao-Nian Yang, Stephen B. Shears, Ingo B. Leibiger, Per-Olof Berggren, Christopher J. Barker
Published in: Biomolecules, Issue 13, 2025, Page(s) 885, ISSN 2218-273X
Publisher: MDPI AG
DOI: 10.3390/biom13060885

Destabilization of β Cell FIT2 by saturated fatty acids alter lipid droplet numbers and contribute to ER stress and diabetes

Author(s): ZHENG X, HO QWC, CHUA M, STELMASHENKO O, YEO XY, MURALIDHARAN S, TORTA F, CHEW EGY, LIAN MM, FOO JN, JUNG S, WONG SH, TAN NS, TONG N, RUTTER AG, WENK MR, SILVER DL, BERGGREN P-O, ALI Y
Published in: Proc Natl Acad Sci, Issue 119, 2022, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2113074119

Apolipoprotein CIII Is an Important Piece in the Type-1 Diabetes Jigsaw Puzzle

Author(s): Ismael Valladolid-Acebes, Per-Olof Berggren, Lisa Juntti-Berggren
Published in: International Journal of Molecular Sciences, Issue 22/2, 2021, Page(s) 932, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22020932

Glucokinase intrinsically regulates glucose sensing and glucagon secretion in pancreatic alpha cells

Author(s): Tilo Moede, Barbara Leibiger, Pilar Vaca Sanchez, Elisabetta Daré, Martin Köhler, Thusitha P. Muhandiramlage, Ingo B. Leibiger, Per-Olof Berggren
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-76863-z

3D‐Printed Biohybrid Microstructures Enable Transplantation and Vascularization of Microtissues in the Anterior Chamber of the Eye

Author(s): Hanie Kavand, Montse Visa, Martin Köhler, Wouter van der Wijngaart, Per‐Olof Berggren, Anna Herland
Published in: Advanced Materials, Issue 36, 2025, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202306686

Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function

Author(s): Francesca Lazzeri-Barcelo, Nuria Oliva-Vilarnau, Marion Baniol, Barbara Leibiger, Olaf Bergmann, Volker M. Lauschke, Ingo B. Leibiger, Noah Moruzzi, Per-Olof Berggren
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-45122-4

Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells

Author(s): ZHAO K, SHI Y, YU J, YU L, MAEL A, LI Y, KOLTON A, JOYCE T, ODORICO J, BERGGREN P-O, YANG S-N
Published in: Cell Transplantation, Issue 31, 2022, Page(s) 1-12, ISSN 0963-6897
Publisher: Cognizant Communication Corp.
DOI: 10.1177/09636897211066508

Human Islet Microtissues as an In Vitro and an In Vivo Model System for Diabetes

Author(s): Joan Mir-Coll, Tilo Moede, Meike Paschen, Aparna Neelakandhan, Ismael Valladolid-Acebes, Barbara Leibiger, Adelinn Biernath, Carina Ämmälä, Ingo B. Leibiger, Burcak Yesildag, Per-Olof Berggren
Published in: International Journal of Molecular Sciences, Issue 22/4, 2021, Page(s) 1813, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22041813

Apolipoprotein CIII Reduction Protects White Adipose Tissues against Obesity-Induced Inflammation and Insulin Resistance in Mice

Author(s): RECIO-LOPEZ P, VALLADOLID-ACEBES I, BERGGREN P-O, JUNTTI-BERGGREN L
Published in: International Journal of Molecular Science, Issue 23, 2022, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23010062

Alpha-to-beta cell crosstalk: Adaptive mechanisms shaping islet function

Author(s): Philip Tröster, Montse Visa, Per-Olof Berggren
Published in: Advances in Biological Regulation, 2025, Page(s) 101121, ISSN 2212-4926
Publisher: Elsevier BV
DOI: 10.1016/j.jbior.2025.101121

The pancreatic islet: a micro-organ in control

Author(s): MH Abdulreda, P-O Berggren
Published in: CellR4, Issue 9, 2021, ISSN 2329-7042
Publisher: Verduci International LLC
DOI: 10.32113/cellr4_20213_3093

Quantitative 3D OPT and LSFM datasets of pancreata from mice with streptozotocin-induced diabetes

Author(s): Max Hahn, Christoffer Nord, Pim P. van Krieken, Per-Olof Berggren, Erwin Ilegems, Abbas Cheddad, Ulf Ahlgren
Published in: Scientific Data, Issue 9, 2022, ISSN 2052-4463
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41597-022-01546-5

Sex-dependent intra-islet structural rearrangements affecting alpha-to-beta cell interactions lead to adaptive enhancements of Ca2+ dynamics in prediabetic beta cells

Author(s): Montse Visa, Per-Olof Berggren
Published in: Diabetologia, Issue 67, 2024, Page(s) 1663-1682, ISSN 0012-186X
Publisher: Springer Verlag
DOI: 10.1007/s00125-024-06173-w

Expression of truncated Kir6.2 promotes insertion of functionally inverted ATP-sensitive K + channels.

Author(s): Benjamin A. Heitz; Robert Bränström; Wei Yang; Yiding Huang; Tilo Moede; Ingo B. Leibiger; Barbara Leibiger; Liu Qi Chen; Jia Yu; Shao-Nian Yang; Olof Larsson; S. Scott Saavedra; Per Olof Berggren; Craig A. Aspinwall
Published in: ACS Nano, Issue 2, 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-00988-y

Novel aspects of intra-islet communication: Primary cilia and filopodia

Author(s): Noah Moruzzi, Barbara Leibiger, Christopher J. Barker, Ingo B. Leibiger, Per-Olof Berggren
Published in: Advances in Biological Regulation, Issue 87, 2025, Page(s) 100919, ISSN 2212-4926
Publisher: Elsevier BV
DOI: 10.1016/j.jbior.2022.100919

Diet-induced obesity promotes endothelial cell desensitization to VEGF-A and permanent islet vessel dysfunction in mice

Author(s): Yan Xiong, Andrea Dicker, Montse Visa, Erwin Ilegems, Per-Olof Berggren
Published in: Journal of Clinical Investigation, Issue 135, 2025, ISSN 1558-8238
Publisher: American Society for Clinical Investigation
DOI: 10.1172/jci177601

Enhanced expression of β cell Ca V 3.1 channels impairs insulin release and glucose homeostasis

Author(s): Jia Yu, Yue Shi, Kaixuan Zhao, Guang Yang, Lina Yu, Yuxin Li, Eva-Marie Andersson, Carina Ämmälä, Shao-Nian Yang, Per-Olof Berggren
Published in: Proceedings of the National Academy of Sciences, Issue 117/1, 2020, Page(s) 448-453, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1908691117

Ectopic leptin production by intraocular islet organoids ameliorates the metabolic phenotype of ob/ob mice

Author(s): Leibiger B, Moede T, Valladolid-Acebes I, Paschen M, Visa M, Leibiger IB, Berggren P-O
Published in: Metabolites, Issue 11, 2021, Page(s) 387, ISSN 2218-1989
Publisher: MDPIST ALBAN-ANLAGE 66, CH-4052 BASEL
DOI: 10.3390/metabo11060387

Islet environment and development of type 1 diabetes in the biobreeding rat model

Author(s): Patricia Recio-López, Per-Olof Berggren, Montserrat Visa Majoral, Ismael Valladolid-Acebes, Lisa Juntti-Berggren
Published in: Signal Transduction and Targeted Therapy, Issue 10, 2025, ISSN 2059-3635
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41392-025-02330-8

Longitudinal monitoring of pancreatic islet damage in streptozotocin-treated mice with optical coherence microscopy

Author(s): Won Yeong Park, Jaeyoon Kim, Hoan Le, Bumju Kim, Per-Olof Berggren, Ki Hean Kim
Published in: Biomedical Optics Express, Issue 14, 2024, Page(s) 54, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.470188

Book chapters (1)

Uncommon Transplantation Sites: Transplantation of Islets and Islet Organoids in the Anterior Chamber of the Eye of Rodents and Monkeys

Author(s): Tilo Moede, Sai Bo Bo Tun, Ingo B. Leibiger, Per-Olof Berggren
Published in: Methods in Molecular Biology, Type-1 Diabetes, 2022, Page(s) 21-36
Publisher: Springer US
DOI: 10.1007/978-1-0716-2807-2_2