Mitochondrial Cristae Biogenesis

Project Information

MitoCRISTAE

Grant agreement ID: 835102

DOI

10.3030/835102

Project closed

EC signature date 12 June 2019

Start date 1 October 2019

End date 30 September 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 286 248,00

EU contribution

€ 2 286 248,00

Coordinated by

UNIVERSITAETSMEDIZIN GOETTINGEN - GEORG-AUGUST-UNIVERSITAET GOETTINGEN - STIFTUNG OEFFENTLICHEN RECHTS
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (29)

Light microscopy of mitochondria at the nanoscale

Author(s): Stefan Jakobs, Till Stephan, Peter Ilgen, Christian Brüser
Published in: Annual review of biophysics, Issue 49, 2020, Page(s) 289-308, ISSN 1936-122X
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-biophys-121219-081550

Structural insights into crista junction formation by the Mic60-Mic19 complex

Author(s): Tobias Bock-Bierbaum, Kathrin Funck, Florian Wollweber, Elisa Lisicki, Karina von der Malsburg, Alexander von der Malsburg, Janina Laborenz, Jeffrey K. Noel, Manuel Hessenberger, Sibylle Jungbluth, Carola Bernert, Séverine Kunz, Dietmar Riedel, Hauke Lilie, Stefan Jakobs, Martin van der Laan, Oliver Daumke
Published in: Science Advances, Issue 8/35, 2022, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.abo4946

MINSTED fluorescence localization and nanoscopy

Author(s): Michael Weber, Marcel Leutenegger, Stefan Stoldt, Stefan Jakobs, Tiberiu S. Mihaila, Alexey N. Butkevich, Stefan W. Hell
Published in: Nature Photonics, Issue 15/5, 2021, Page(s) 361-366, ISSN 1749-4885
Publisher: Nature Pub. Group
DOI: 10.1038/s41566-021-00774-2

MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation.

Author(s): Stephan T, Brüser C, Deckers M, Steyer AM, Balzarotti F, Barbot M, Behr TS, Heim G, Hübner W, Ilgen P, Lange F, Pacheu-Grau D, Pape JK, Stoldt S, Huser T, Hell SW, Möbius W, Rehling P, Riedel D, Jakobs S
Published in: The EMBO Journal, Issue 39/14, 2020, ISSN 1460-2075
Publisher: EMBO journal
DOI: 10.15252/embj.2019104105

An aldehyde-crosslinking mitochondrial probe for STED imaging in fixed cells

Author(s): Jingting Chen, Till Stephan, Felix Gaedke, Tianyan Liu, Yiyan Li, Astrid Schauss, Peng Chen, Veronika Wulff, Stefan Jakobs, Christian Jüngst, Zhixing Chen
Published in: Proceedings of the National Academy of Sciences, Issue 121, 2025, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2317703121

Monitoring mitochondrial translation in living cells.

Author(s): Roya Yousefi Eugenio F Fornasiero Lukas Cyganek Julio Montoya Stefan Jakobs Silvio O Rizzoli Peter Rehling David Pacheu‐Grau
Published in: EMBO Reports, 2021, ISSN 1469-3178
Publisher: EMBO Press
DOI: 10.15252/embr.202051635

Drosophila MIC10b can polymerize into cristae-shaping filaments

Author(s): Till Stephan Stefan Stoldt Mariam Barbot Travis D Carney Felix Lange Mark Bates Peter Bou Dib Kaushik Inamdar Halyna R Shcherbata Michael Meinecke Dietmar Riedel Sven Dennerlein Peter Rehling Stefan Jakobs
Published in: Life Science Alliance, 2024, ISSN 2575-1077
Publisher: Life Science Alliance LLC
DOI: 10.26508/lsa.202302177

MultiMatch: geometry-informed colocalization in multi-color super-resolution microscopy

Author(s): Julia Naas, Giacomo Nies, Housen Li, Stefan Stoldt, Bernhard Schmitzer, Stefan Jakobs, Axel Munk
Published in: Communications Biology, Issue 7, 2024, ISSN 2399-3642
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s42003-024-06772-8

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors

Author(s): Sabine Poerschke; Sven Dennerlein; Silke Oeljeklaus; Cong Wang; Ricarda Richter-Dennerlein; Johannes Sattmann; Diana Bauermeister; Elisa Hanitsch; Stefan Stoldt; Thomas Langer; Stefan Jakobs; Bettina Warscheid; Peter Rehling
Published in: eLife, Issue 1, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.68213

Decisive role of mDia-family formins in cell cortex function of highly adherent cells

Author(s): Jonas Scholz, Till Stephan, Aina Gallemí Pérez, Agnes Csiszár, Nils Hersch, Lisa S. Fischer, Stefan Brühmann, Sarah Körber, Christof Litschko, Lucija Mijanovic, Thomas Kaufmann, Felix Lange, Ronald Springer, Andreas Pich, Stefan Jakobs, Michelle Peckham, Marco Tarantola, Carsten Grashoff, Rudolf Merkel, Jan Faix
Published in: Science Advances, Issue 10, 2024, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adp5929

3D Computational Modeling of Defective Early Endosome Distribution in Human iPSC-Based Cardiomyopathy Models

Author(s): Hafiza Nosheen Saleem, Nadezda Ignatyeva, Christiaan Stuut, Stefan Jakobs, Michael Habeck, Antje Ebert
Published in: Cells, Issue 13, 2025, Page(s) 923, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells13110923

Super-resolution microscopy of mitochondrial mRNAs

Author(s): Stefan Stoldt, Frederike Maass, Michael Weber, Sven Dennerlein, Peter Ilgen, Jutta Gärtner, Aysenur Canfes, Sarah V. Schweighofer, Daniel C. Jans, Peter Rehling, Stefan Jakobs
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-61577-5

Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins

Author(s): Jasmin K. Pape, Till Stephan, Francisco Balzarotti, Rebecca Büchner, Felix Lange, Dietmar Riedel, Stefan Jakobs, Stefan W. Hell
Published in: Proceedings of the National Academy of Sciences, Issue 117/34, 2020, Page(s) 20607-20614, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2009364117

Colocalization for super-resolution microscopy via optimal transport

Author(s): Carla Tameling, Stefan Stoldt, Till Stephan, Julia Naas, Stefan Jakobs, Axel Munk
Published in: Nature Computational Science, 2021, ISSN 2662-8457
Publisher: Nature
DOI: 10.1038/s43588-021-00050-x

DNA-PAINT MINFLUX nanoscopy.

Author(s): Lynn M. Ostersehlt Daniel C. Jans Anna Wittek Jan Keller-Findeisen Kaushik Inamdar Steffen J. Sahl Stefan W. Hell Stefan Jakobs
Published in: Nature Methods, 2022, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-022-01577-1

Deep learning enables fast, gentle STED microscopy

Author(s): Vahid Ebrahimi Till Stephan Jiah Kim Pablo Carravilla Christian Eggeling Stefan Jakobs Kyu Young Han
Published in: communications biology, 2023, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-023-05054-z

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain

Author(s): Tianyan Liu; Till Stephan; Peng Chen; Jan Keller-Findeisen; Jingting Chen; Dietmar Riedel; Zhongtian Yang; Stefan Jakobs; Zhixing Chen
Published in: Proceedings of the National Academy of Sciences of the United States of America, Issue 2, 2022, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2215799119

In situ architecture of the human prohibitin complex

Author(s): Felix Lange, Michael Ratz, Jan-Niklas Dohrke, Maxence Le Vasseur, Dirk Wenzel, Peter Ilgen, Dietmar Riedel, Stefan Jakobs
Published in: Nature Cell Biology, Issue 27, 2025, Page(s) 633-640, ISSN 1465-7392
Publisher: Nature Publishing Group
DOI: 10.1038/s41556-025-01620-1

Silencing mitochondrial gene expression in living cells

Author(s): Luis Daniel Cruz-Zaragoza, Drishan Dahal, Mats Koschel, Angela Boshnakovska, Aiturgan Zheenbekova, Mehmet Yilmaz, Marcel Morgenstern, Jan-Niklas Dohrke, Julian Bender, Anusha Valpadashi, Kristine A. Henningfeld, Silke Oeljeklaus, Laura Sophie Kremer, Mirjam Breuer, Oliver Urbach, Sven Dennerlein, Michael Lidschreiber, Stefan Jakobs, Bettina Warscheid, Peter Rehling
Published in: Science, Issue 389, 2025, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.adr3498

The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy

Author(s): Daniel Stumpf, Nickels Jensen, Cédric Mittelheisser, Jan Keller-Findeisen, Alexey I. Chizhik, Maria Kamper, Timo Diekmann, Florian Habenstein, Isabelle Jansen, Jörg Enderlein, Michel Sliwa, Kaushik Inamdar, Stefan W. Hell, Stefan Jakobs
Published in: Proceedings of the National Academy of Sciences, Issue 122, 2025, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2504748122

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase

Author(s): Praveenraj Elancheliyan, Klaudia K. Maruszczak, Remigiusz Adam Serwa, Till Stephan, Ahmet Sadik Gulgec, Mayra A. Borrero-Landazabal, Sonia Ngati, Aleksandra Gosk, Stefan Jakobs, Michal Wasilewski, Agnieszka Chacinska
Published in: Cell Reports, Issue 43, 2025, Page(s) 115038, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2024.115038

Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models

Author(s): Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hüper, Till Stephan, Peter Ilgen, Angel R. Cereceda Delgado, Elisa D’Este, Alexander Egner, Stefan Jakobs, Steffen J. Sahl, Stefan W. Hell
Published in: Nature Methods, Issue 19, 2022, Page(s) 603-612, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-022-01465-8

The TFAM-to-mtDNA ratio defines inner-cellular nucleoid populations with distinct activity levels

Author(s): Christian Brüser, Jan Keller-Findeisen, Stefan Jakobs
Published in: Cell Reports, 2021, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2021.110000

Developmental changes of the mitochondria in the murine anteroventral cochlear nucleus

Author(s): Hintze, A.; Lange, F.; Steyer, A.; Anstatt, J.; Möbius, W.; Jakobs, S. ; https://orcid.org/0000-0002-8028-3121; Wichmann, C.
Published in: iScience, Issue 2, 2024, ISSN 2589-0042
Publisher: Elsevier
DOI: 10.1016/j.isci.2023.108700

Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria

Author(s): Sarah V. Schweighofer, Daniel C. Jans, Jan Keller-Findeisen, Anne Folmeg, Peter Ilgen, Mark Bates, Stefan Jakobs
Published in: Cell Death & Differentiation, Issue 31, 2024, Page(s) 469-478, ISSN 1350-9047
Publisher: Nature Publishing Group
DOI: 10.1038/s41418-024-01273-x

Mitochondrial Protein Abundance Gradients Require the Distribution of Separated Mitochondria.

Author(s): Franziska Bollmann; Jan-Niklas Dohrke; Jan-Niklas Dohrke; Christian A. Wurm; Daniel C. Jans; Daniel C. Jans; Stefan Jakobs; Stefan Jakobs; Stefan Jakobs
Published in: Biology (Basel), Issue 1, 2021, ISSN 2079-7737
Publisher: Basel: MDPI AG
DOI: 10.3390/biology10070572

Mitochondrial dysfunction and impaired oxidative stress defense as potential trigger of cerebral X-linked adrenoleukodystrophy

Author(s): L.M. Marten, M.S. Lüttgens, B. Berečić, M. Tiburcy, C. Brüser, S. Gupta, S. Jakobs, E. Ammer, A. Ohlenbusch, R. Krätzner, H. Rosewich, J. Gärtner
Published in: Free Radical Biology and Medicine, Issue 242, 2025, Page(s) 654-666, ISSN 0891-5849
Publisher: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2025.10.289

Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation

Author(s): Benedikt S. Saller, Svenja Wöhrle, Larissa Fischer, Clara Dufossez, Isabella L. Ingerl, Susanne Kessler, Maria Mateo-Tortola, Oliver Gorka, Felix Lange, Yurong Cheng, Emilia Neuwirt, Adinarayana Marada, Christoph Koentges, Chiara Urban, Philipp Aktories, Peter Reuther, Sebastian Giese, Susanne Kirschnek, Carolin Mayer, Johannes Pilic, Hugo Falquez-Medina, Aline Oelgeklaus, Veerasikku Gopal Deepag
Published in: Immunity, Issue 58, 2025, Page(s) 90-107.e11, ISSN 1074-7613
Publisher: Cell Press
DOI: 10.1016/j.immuni.2024.10.012

A data-driven model for mitochondrial inner membrane remodeling as a driving force of organelle shaping

Author(s): Noga Preminger, Ben Zucker, Sarah Hassdenteufel, Till Stephan, Stefan Jakobs, Michael M. Kozlov, Maya Schuldiner
Published in: Journal of Cell Science, Issue 138, 2025, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.263850

Non-peer reviewed articles (9)

MINSTED fluorescence localization and nanoscopy

Author(s): Michael Weber, Marcel Leutenegger, Stefan Stoldt, Stefan Jakobs, Tiberiu S. Mihaila, Alexey N. Butkevich, Stefan W. Hell
Published in: BioRxiv, 2024, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2020.10.31.363424

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain

Author(s): Tianyan Liu, Till Stephan, Peng Chen, Jingting Chen, Dietmar Riedel, Zhongtian Yang, Stefan Jakobs, Zhixing Chen
Published in: BioRxiv, Issue 26928205, 2022, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.05.09.491019

In-situ architecture of the human prohibitin complex

Author(s): Felix Lange, Michael Ratz, Jan-Niklas Dohrke, Dirk Wenzel, Peter Ilgen, Dietmar Riedel, Stefan Jakobs
Published in: BioRxiv, 2025, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.02.14.579514

The Drosophila MIC10 orthologue has a propensity to polymerize into cristae-shaping filaments

Author(s): Till Stephan, Stefan Stoldt, Mariam Barbot, Travis D. Carney, Felix Lange, Mark Bates, Peter Bou Dib, Halyna R. Shcherbata, Michael Meinecke, Dietmar Riedel, Sven Dennerlein, Peter Rehling, Stefan Jakobs
Published in: BioRxiv, 2023, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.04.17.537183

Optimal Precision and Accuracy in 4Pi-STORM using Dynamic Spline PSF Models

Author(s): Mark Bates, Jan Keller-Findeisen, Adrian Przybylski, Andreas Hüper, Till Stephan, Peter Ilgen, Angel R. Cereceda Delgado, Elisa D’Este, Stefan Jakobs, Steffen J. Sahl, Stefan W. Hell
Published in: BioRxiv, 2022, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2021.10.19.464803

Super-resolution microscopy of mitochondrial mRNAs

Author(s): Stefan Stoldt, Frederike Maass, Michael Weber, Sven Dennerlein, Peter Ilgen, Jutta Gärtner, Aysenur Canfes, Sarah V. Schweighofer, Daniel C. Jans, Peter Rehling, Stefan Jakobs
Published in: BioRxiv, 2025, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.01.23.634455

Endogenous BAX and BAK form mosaic rings of variable size and composition on apoptotic mitochondria

Author(s): Sarah Vanessa Schweighofer, Daniel C. Jans, Jan Keller-Findeisen, Anne Folmeg, Mark Bates, Stefan Jakobs
Published in: BioRxiv, 2025, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.08.18.553869

Structural insights into crista junction formation by the Mic60-Mic19 complex

Author(s): Tobias Bock-Bierbaum, Kathrin Funck, Florian Wollweber, Elisa Lisicki, Janina Laborenz, Jeffrey K. Noel, Manuel Hessenberger, Alexander von der Malsburg, Karina von der Malsburg, Carola Bernert, Séverine Kunz, Dietmar Riedel, Hauke Lilie, Stefan Jakobs, Martin van der Laan, Oliver Daumke
Published in: BioRxiv, 2022, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.03.30.486340

MultiMatch: Geometry-Informed Colocalization in Multi-Color Super-Resolution Microscopy

Author(s): Julia Naas, Giacomo Nies, Housen Li, Stefan Stoldt, Bernhard Schmitzer, Stefan Jakobs, Axel Munk
Published in: BioRxiv, 2025, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.02.28.581557