Periodic Reporting for period 2 - SYNPEP (Synthetic biology of non-ribosomal peptide synthetases to generate new peptides)
Période du rapport: 2021-04-01 au 2022-09-30
Within SYNPEP we apply fast and high-throughput methods to find new NRPS systems in bacteria, activate the underlying biosynthetic gene clusters (BGCs), use the identified NRPS fragments to generate novel peptides based on NRPS engineering approaches and produce these products in amounts sufficient for bioactivity testing and/or chemical modification.
The latter is facilitated by new-to-nature NRPS systems that accept unusual and chemically reactive building blocks for targeted modifications.
Within SYNPEP we continuously develop and improve our NRPS engineering methods that now allow to produce thousands (later millions) of novel peptides. These are then screened for their bioactivity in clinical relevant assays based on microfluidics coupling the bacterial production of these peptides directly to their bioassay.
The ultimate goal is to make NRPS engineering accessible to even non-experts in the field. For commercial application the idea is to start a company with a focus on NRPS engineering.