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The Impact of Callose Metabolism on the Mechanical Properties of Cell Wall during Tomato Ripening .

Project description

Investigating the role of Callose in tomato ripening

The tomato is considered one of the most important crops due to its nutrient properties. As a soft fruit, it is characterised by a rapid and high loss of firm texture. More resistant varieties could prove instrumental for optimising production, processes and transports. Research has implicated the beta 1.3 glucan component (callose) in the degradation of fruit texture. The EU-funded CallMechanics project intends to investigate the role callose plays in fruit softening to determine the impact changes in callose accumulation have on the latest stages of fruit production. The result will lead to new molecular instruments to apply in the selection and cultivation of more resistant varieties.

Objective

Tomato, SolanTomato, Solanum lycopersicum L. is one of the most important crops and an important source of nutrients in the world. However, around 25-42% of the yield are lost during postharvest. Rapid softening is one of the main causes reducing the shelf-life of the fruit. Therefore, delaying this process is one of the major targets in fruit breeding programmes.
Evidence show that stimulating callose in tomatoes via bruising or heating induce changes in fruit texture. In this project, the impact on fruit softening of cell wall modifications targeting the synthesis/degradation of the beta 1,3 glucan component (named callose) will be investigated. The aim is to determine how changes in callose accumulation at the latest stages of fruit development modify the texture, the structural and mechanical properties of tomato fruit. Introgression and transgenic lines with modified callose metabolism will be generated and cell wall biochemistry and mechanical properties will be characterized combining cross-disciplinary approaches such as immunolocalization, Fourier-transformed infrared, nano and macro indentation and Raman spectroscopy. Moreover, the impact of callose modifications on plant/fruit development and on other processes related to softening (such as water content or pathogen susceptibility) will be determined.
The results of our project will provide novel molecular tools to use in the selection and breeding of fruit varieties. As a direct outcome, we expect to obtain plants producing fruits that maintain their firmness for a longer period of time, thus with reduce susceptibility to mechanical damage and pathogen attack during the postharvest period. This achievement will have a positive impact on the European Union economy by optimizing processes such as the frequency of harvesting, the handling and the transport procedures. It will also impact on human health and food security by reducing losses and the spreading of pathogens that thrive in mature soft fruits.

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

(opens in new window) H2020-MSCA-IF-2018

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Coordinator

UNIVERSITY OF LEEDS
Net EU contribution
€ 212 933,76
Address
WOODHOUSE LANE
LS2 9JT Leeds
United Kingdom
Region
Yorkshire and the Humber West Yorkshire Leeds
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 212 933,76