Biocatalytic Methyltransferase Cascades

Información del proyecto

MetTraC

Identificador del acuerdo de subvención: 840243

DOI

10.3030/840243

Proyecto cerrado

Fecha de la firma de la CE 4 Febrero 2019

Fecha de inicio 1 Marzo 2019

Fecha de finalización 31 Agosto 2020

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 149 760,00

Aportación de la UE

€ 149 760,00

Coordinado por

UNIVERSITAT BASEL
Switzerland

Periodic Reporting for period 1 - MetTraC (Biocatalytic Methyltransferase Cascades)

Período documentado: 2019-03-01 hasta 2020-08-31

Resumen del contexto y de los objetivos generales del proyecto

Biocatalysis may provide a more sustainable approach to making complex molecules than traditional chemical synthesis. As the discovery of novel enzyme classes progresses, the biocatalytic toolbox is becoming more comprehensive, suggesting that enzyme-based synthesis of complex artificial molecules is becoming a realistic perspective for industrial production. Enzymes that catalyze group-transfer reactions depend on complex co-substrates – such as S-adenosylmethionine-dependent methyltransferases (SAM) – which must be regenerated after every turnover. This dependence makes the application of methyltransferases for preparative reactions difficult. In this project we showed this this problem can be circumvented by introducing a robust cofactor-regeneration system. As a proof-of-concept we developed and optimized enzyme-catalyzed cascade reactions that enable the one-pot synthesis of natural products and non-natural derivatives thereof.