Biocatalytic Methyltransferase Cascades

Projektinformationen

MetTraC

ID Finanzhilfevereinbarung: 840243

DOI

10.3030/840243

Projekt abgeschlossen

EK-Unterschriftsdatum 4 Februar 2019

Startdatum 1 März 2019

Enddatum 31 August 2020

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten

€ 149 760,00

EU-Beitrag

€ 149 760,00

Koordiniert durch

UNIVERSITAT BASEL
Switzerland

Periodic Reporting for period 1 - MetTraC (Biocatalytic Methyltransferase Cascades)

Berichtszeitraum: 2019-03-01 bis 2020-08-31

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Biocatalysis may provide a more sustainable approach to making complex molecules than traditional chemical synthesis. As the discovery of novel enzyme classes progresses, the biocatalytic toolbox is becoming more comprehensive, suggesting that enzyme-based synthesis of complex artificial molecules is becoming a realistic perspective for industrial production. Enzymes that catalyze group-transfer reactions depend on complex co-substrates – such as S-adenosylmethionine-dependent methyltransferases (SAM) – which must be regenerated after every turnover. This dependence makes the application of methyltransferases for preparative reactions difficult. In this project we showed this this problem can be circumvented by introducing a robust cofactor-regeneration system. As a proof-of-concept we developed and optimized enzyme-catalyzed cascade reactions that enable the one-pot synthesis of natural products and non-natural derivatives thereof.