Project description DEENESFRITPL DNA-based antibody detection platforms to facilitate diagnosis Antibody detection plays an indispensable role in the diagnosis of many health conditions, including infectious, autoimmune and oncological diseases. The EU-funded DNA-NANO-AB project is proposing the development of DNA-based, rapid, user-friendly, sensitive and low-cost electrochemical nanodevices for the detection of clinically relevant antibodies that could be introduced as point-of-care applications. The project will achieve this through the rational design and fabrication of disposable DNA-based sensor platforms, where the detection of specific antibodies will be amplified via an electrochemical read-out approach. The developed platforms will be characterised, optimised and tested using immunoglobulin levels in real representative samples. Show the project objective Hide the project objective Objective WHY: Antibody detection plays a pivotal role in the diagnosis of many human disorders including infectious, autoimmune, and oncological diseases. Unfortunately, current methods routinely used for the detection of these biomarkers still require sophisticated equipment, washing intensive, reagent-intensive multi-step processes. In attempt to overcome the cited limitations, it’s mandatory find new approaches for the sensitive detection of antibodies. WHAT: DNA-NANO-AB proposes the development of DNA-based novel, rapid, user friendly, robust, sensitive and low cost electrochemical NANOdevices for orthogonal multiplex detection of antibodies that may be advantageous over existing methods for the detection of clinically-relevant antibodies and could be well-positioned for point-of-care applications. I will achieve this goal by rationally designing and fabricating disposable DNA-based sensing platforms in which the detection of specific antibodies will be signaled, and amplified, through an electrochemical read-out approach. The developed platforms will be characterized, optimized and then tested for the dosage of immunoglobulins levels directly in real representative samples. HOW: During the fellowship I will undertake a training program to develop skills and acquire expertise in molecular biology, electrochemical biosensor and DNA Nanotechnology. To achieve these objectives, I will combine the different scientific backgrounds and expertise of two leading scientists: Prof. Plaxco (University of California Santa Barbara), a world-wide expert in electrochemical biosensors, and Prof. Ricci (University of Rome, Tor Vergata), a young researcher active at the forefront of DNA nanotechnology for diagnostic and drug-delivery applications. Thanks to this MSC Fellowship, I will diversify my scientific knowledge and acquire cutting-edge competences as well as complementary skills, which will become the foundation of my scientific independence. Fields of science engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsnatural scienceschemical scienceselectrochemistrynatural sciencesbiological sciencesgeneticsDNAengineering and technologynanotechnologynatural sciencesbiological sciencesmolecular biology Keywords Electrochemical biosensors electrochemical DNA sensors reagentless biosensors disposable electrochemical devices aptamers DNA Nanotechnology DNA nanodevices Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2018 - Individual Fellowships Call for proposal H2020-MSCA-IF-2018 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA Net EU contribution € 251 002,56 Address VIA CRACOVIA 50 00133 Roma Italy See on map Region Centro (IT) Lazio Roma Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 251 002,56 Partners (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. THE REGENTS OF THE UNIVERSITY OF CALIFORNIA United States Net EU contribution € 0,00 Address FRANKLIN STREET 1111 12 FLOOR 94607 OAKLAND CA See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 165 265,92