superbright, photostabLe and mUlticolour novel fluoresCent mEtal quaNtum clusters for super-resoluTion imaging

Descripción del proyecto

Mejores marcadores fluorescentes con los que obtener imágenes a nanoescala con más resolución

La microscopía de superresolución ha revolucionado la biología y la nanomedicina, y permite visualizar estructuras biológicas o sintéticas complejas con una resolución nanométrica. Las técnicas de superresolución se basan en marcadores fluorescentes con propiedades específicas, como el ajuste óptico, la unión reversible y la emisión estimulada. A pesar de los avances en el campo, el desarrollo de sondas fluorescentes con alto brillo y fotoestabilidad, así como con baja toxicidad, es un desafío. El proyecto LUCENT, financiado por las Acciones Marie Skłodowska-Curie, se propone combinar estrategias nanoquímicas y de ingeniería de superficies para producir grupos de metales atómicos multicolores para su uso en microscopía de superresolución. El trabajo del proyecto podría dar lugar a imágenes tridimensionales en vivo, en colores y con una resolución aún mayor, hasta la escala molecular.

Objetivo

The breakthrough of super resolution imaging has revolutionized biology and nanomedicine, allowing to visualize synthetic or biological structures with nanometric accuracy and providing a deeper understanding of complex molecular systems. The key idea at the basis of these methods is the use of novel fluorescent markers endowed with specific properties such as photoswitching, reversible binding, stimulated emission and single molecule imaging capabilities. In this emergent framework the fluorescent markers play a role as important as the optical setup utilized for imaging. Therefore, among the prerequisites for successful nanoscopic imaging the selection of the suitable fluorophore is essential. Despite the recent advances in the development of super-resolution microscopy techniques, fluorescent probes with high brightness, high photostability and low toxicity remains an unmet need. This proposal aims at developing ultrasmall photoluminescent atomic metal quantum clusters as new generation of advanced optical probes for super-resolution microscopy. To this purpose, we will apply a combination of property-designed nanochemistry routes and surface engineered strategies for producing multicolour atomic metal clusters that will provide novel model systems for single molecule imaging. The strategy will focus at three important levels: synthesis, biocompatibility and preliminary cellular imaging, application in super-resolution microscopy. The research activity here proposed could realistically lead to live, multicolour, three-dimensional images with an even higher resolution – down to the molecular level – with applications in biology and nanotechnology. To this end, LUCENT will employ a multidiscicplinary approach whose strength resides in the combination of the complementary expertise of the experienced researcher –design of new probes - and the host institution - nanoscopy.