Objective One of the key goals to understand GBM biology and find strategies to treat this deadly tumor is to define the different cell populations within the tumor. The inter-tumor heterogeneity among GBM patients, investigated on a molecular level, has led to the identification of three main subtypes; the proneural (PN), mesenchymal (MES), and classical (CL), which are associated with specific mutations. The different properties of the three subgroups play an important role in the clinical response and the pathology of the tumor. The tumor microenvironment, that play an important role in tumor heterogeneity, is built up by multiple factors including recruited cells, blood vessels, secreted cytokines, and extracellular matrix. However, the constitution of these factors in each subtype is not known. In this work, we will characterize the cells that are recruited to the different GBM subtypes focusing on tumor-associated macrophages (TAMs) and mesenchymal stromal cells (MSCs). For this purpose, transgenic mice representing the three glioma subtypes will be used. Chimeric mice will be utilized to investigate the origin of the recruited cells. Flow cytometry, confocal analysis, multiplex assays and single-cell gene expression analysis will be used to characterize the tumor cells and the recruited cells to find master regulatory elements of recruitment. Therapeutic targeting will follow identification of master regulatory factors that recruit TAMs and MSCs to the tumor. Fields of science natural sciencesbiological sciencesgeneticsmutationmedical and health sciencesbasic medicinepathology Keywords Glioblastoma GBM Tumor stroma microenvironment tumor-associated macrophages TAM mesenchymal stromal cells MSCs Tumor subtypes proneural mesenchymal classical genetic mouse models Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2018 - Individual Fellowships Call for proposal H2020-MSCA-IF-2018 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator KAROLINSKA INSTITUTET Net EU contribution € 261 192,00 Address Nobels vag 5 17177 Stockholm Sweden See on map Region Östra Sverige Stockholm Stockholms län Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Participants (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all GOETEBORGS UNIVERSITET Participation ended Sweden Net EU contribution € 0,00 Address Vasaparken 405 30 Goeteborg See on map Region Södra Sverige Västsverige Västra Götalands län Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Partners (2) Sort alphabetically Sort by Net EU contribution Expand all Collapse all Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. EMORY UNIVERSITY NON PROFIT CORP Participation ended United States Net EU contribution € 0,00 Address Clifton road, ne, 4th floor 1599 30322 Atlanta See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Partner Partner organisations contribute to the implementation of the action, but do not sign the Grant Agreement. ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI United States Net EU contribution € 0,00 Address One gustave l levy place box 1075 10029 6574 New york See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 165 265,92
