Synthetic Oligonucleotide Quality Control Software

Project Information

SOLQC

Grant agreement ID: 851018

DOI

10.3030/851018

Project closed

EC signature date 6 June 2019

Start date 1 July 2019

End date 30 June 2020

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 100 000,00

EU contribution

€ 100 000,00

Coordinated by

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

Periodic Reporting for period 1 - SOLQC (Synthetic Oligonucleotide Quality Control Software)

Reporting period: 2019-07-01 to 2020-06-30

Summary of the context and overall objectives of the project

In this work we present SOLQC, a software tool that supports and potentially standardize the statistical analysis and quality control of OLs. The tool is designed to facilitate analysis by individual labs to obtain information about DNA libraries and to perform error analysis before or during experiments.
The aim of SOLQC was to develop a reliable quality control software for oligonucleotide libraries. Furthermore – we aimed to bring this software tool to a technical level that can position it to be possibly adapted by commercial partners/entities. The final result of this work is novel analysis tool, enabling fast and comprehensive quality analysis of synthetic oligo libraries, based on NGS data. SOLQC provides detailed statistics, information and insights about the state and quality of the library, to the user, and allows for more informed decisions on next stages of the OL experiment.

Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far

We demonstrate SOLQC by analyzing literature libraries. We also discuss the potential benefits and relevance of the different components of the analysis.
SOLQC now has a functioning website that allows for the complete analysis.
The tool is published as a paper in Bioinformatics, a leading journal in the field, read by academia and industry leaders (doi:10.1093/bioinformatics/btaa740).
SOLQC was presented in central venues of the DNA storage and synthetic biology communities. Initial contacts with a commercial partner were formed. Following the publication in Bionformatics we expect more interest in using SOLQC, from OL users in the industry, the healthcare sector and in academia.

Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far)

We expect increased interest from academia and the private sector in the coming year, as the usage of oligo-libraries become more wide-spread within the biological community.

solqc-300x180.png