Synthetic Oligonucleotide Quality Control Software

Projektinformationen

SOLQC

ID Finanzhilfevereinbarung: 851018

DOI

10.3030/851018

Projekt abgeschlossen

EK-Unterschriftsdatum 6 Juni 2019

Startdatum 1 Juli 2019

Enddatum 30 Juni 2020

Finanziert unter

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Gesamtkosten

€ 100 000,00

EU-Beitrag

€ 100 000,00

Koordiniert durch

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Israel

Periodic Reporting for period 1 - SOLQC (Synthetic Oligonucleotide Quality Control Software)

Berichtszeitraum: 2019-07-01 bis 2020-06-30

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

In this work we present SOLQC, a software tool that supports and potentially standardize the statistical analysis and quality control of OLs. The tool is designed to facilitate analysis by individual labs to obtain information about DNA libraries and to perform error analysis before or during experiments.
The aim of SOLQC was to develop a reliable quality control software for oligonucleotide libraries. Furthermore – we aimed to bring this software tool to a technical level that can position it to be possibly adapted by commercial partners/entities. The final result of this work is novel analysis tool, enabling fast and comprehensive quality analysis of synthetic oligo libraries, based on NGS data. SOLQC provides detailed statistics, information and insights about the state and quality of the library, to the user, and allows for more informed decisions on next stages of the OL experiment.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

We demonstrate SOLQC by analyzing literature libraries. We also discuss the potential benefits and relevance of the different components of the analysis.
SOLQC now has a functioning website that allows for the complete analysis.
The tool is published as a paper in Bioinformatics, a leading journal in the field, read by academia and industry leaders (doi:10.1093/bioinformatics/btaa740).
SOLQC was presented in central venues of the DNA storage and synthetic biology communities. Initial contacts with a commercial partner were formed. Following the publication in Bionformatics we expect more interest in using SOLQC, from OL users in the industry, the healthcare sector and in academia.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

We expect increased interest from academia and the private sector in the coming year, as the usage of oligo-libraries become more wide-spread within the biological community.

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