Description du projet
L’obésité infantile et le développement neuronal
L’obésité constitue un fardeau mondial, et l’obésité infantile est reconnue comme un grave problème de santé. Le projet ONTOGENY, financé par l’UE, vise à découvrir les processus développementaux qui gouvernent le contrôle de l’homéostasie énergétique. Les neuropeptides hypothalamiques apparentés à agouti (AgRP) sont susceptibles de stimuler l’apport alimentaire, tandis que les neurones à proopiomélanocortine (POMC) inhibent le comportement alimentaire. Le projet entend étudier l’ontogénie tardive des neurones AgRP et POMC, en mesurant leur activité lors du développement des souris, en réponse à des nutriments et à des hormones impliqués dans l’homéostasie énergétique. En définitive, il permettra de dévoiler des informations importantes concernant le développement des neurones AgRP et POMC dans des conditions pertinentes pour l’obésité et la dérégulation métabolique de l’enfant.
Objectif
Obesity is a significant global burden that is associated with adverse health outcomes. Adults are not alone in their struggles with obesity. Children now accumulate fat at an alarming rate, making childhood obesity a major health problem. Unfortunately, we do not know enough about the pathophysiology of these conditions to propose appropriate prevention strategies and more effective therapeutic approaches. In this ERC StG proposal, our goal is to discover the developmental processes important for proper con-trol of energy homeostasis. We will study Agrp and POMC neurons, located in the arcuate nucleus of the hypothalamus, that are heavily involved with control of energy homeostasis. Agrp and POMC neu-rons have delayed postnatal development, maturing their axonal projections around the third postnatal week in rodents. Here, we will study in detail this late ontogeny of Agrp and POMC neurons. In Aim 1, we will use novel approaches to measure Agrp and POMC neuronal activity during mouse development in response to nutrients and hormones involved in energy homeostasis. In Aim 2, we will use whole-brain imaging techniques to determine the anatomical development of Agrp and POMC neuronal projec-tions. We will also study the importance of a specific neuronal circuit in neonates to the development of obesity. Finally, in Aim 3, we will identify critical molecular mechanisms involved in the ontogeny of Agrp and POMC neurons by investigating their translatome over the course of postnatal development. Overall, this project will provide novel insights into Agrp and POMC neuron development in conditions relevant to childhood obesity and metabolic dysregulation. The functional, anatomical and molecular mechanisms illuminated here will provide the foundation for future studies aimed to dissect the devel-opment of other homeostatic systems.
Champ scientifique
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Régime de financement
ERC-STG - Starting GrantInstitution d’accueil
OX1 2JD Oxford
Royaume-Uni