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CORDIS

ASsembly and phase Transitions of Ribonucleoprotein Aggregates in neurons: from physiology to pathology.

Project description

RNA and ribonucleoprotein aggregates in neurons

Recent studies implicate the pathogenic relevance of altered RNA metabolism and aberrant ribonucleoprotein (RNP) assembly in several neurodegenerative diseases. How defective RNPs form, what their integral components are and which events trigger their appearance late in life are still unsolved issues. The EU-funded ASTRA project will combine sophisticated imaging-derived RNP complex purification, genetic tools and innovative computational approaches to investigate the biophysical properties and composition of the complexes and how they are modified in disease conditions. The development of new imaging and optical methods will allow scientists to study the separation of RNPs in liquid and solid phases in cells, in tissues and animal models, and to characterise their RNA and protein components in physiological and pathological states.

Objective

Recent works indicate the pathogenic relevance of altered RNA metabolism and aberrant ribonucleoprotein (RNP) assembly in several neurodegenerative diseases, such as Amyotrophic lateral sclerosis. How defective RNPs form, what are their integral components and which events trigger their appearance late in life are still unsolved issues. While emerging evidence indicates that mutations and post-translational modifications of specific RNA-binding proteins (RBPs) induce liquid-solid phase transition in vitro, much less is known about the in vivo properties of RNP assemblies and which role RNA plays in their formation.
ASTRA will combine sophisticated imaging-derived RNP complex purification with innovative computational approaches and powerful genetic tools to unravel the biophysical properties and composition of RBP complexes and how they are modified in disease conditions. Through the development of new imaging and optical methods we plan to study how RNPs separate in liquid and solid phases in cells, in tissues (retina) and animal models and to characterize their RNA and protein components in physiological and pathological states.
Exploiting the novel finding that non-coding RNAs act as scaffolding molecules for RNP assembly, we will investigate how such RNAs control the dynamic link between RNP formation, intracellular sorting and function. In a genuine interdisciplinary team effort, we will reveal how the architecture and localization of cytoplasmic RNP complexes are controlled in motor neurons and affected in neurodegeneration.
We plan to develop novel advanced microscopy methods to monitor formation of aberrant RNPs in vivo and we will explore new molecules to impede pathological cascades driven by RNP assemblies. In conclusion, ASTRA will allow us to gain a comprehensive understanding of RNP function and dysfunction; we will use this knowledge to develop new therapeutic strategies that will impact on several protein-misfolding neurodegenerative diseases.

Host institution

UNIVERSITA DEGLI STUDI DI ROMA LA SAPIENZA
Net EU contribution
€ 2 138 904,45
Address
Piazzale Aldo Moro 5
00185 Roma
Italy

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Region
Centro (IT) Lazio Roma
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 2 138 904,45

Beneficiaries (2)