Descripción del proyecto
La medición simultánea de los potenciales de acción de miles de células agiliza las pruebas de seguridad cardíaca
El cribaje de alto rendimiento ha revolucionado el descubrimiento de fármacos y los ensayos de toxicidad. Sin embargo, determinar la seguridad cardíaca de posibles fármacos todavía sigue siendo una tarea laboriosa y cara que empieza con ensayos en líneas celulares, seguidos de simulaciones informáticas y acaba con ensayos en humanos. El proyecto MAREP, financiado con fondos europeos, prevé ofrecer una alternativa rápida, automatizada y asequible. Los científicos del proyecto han desarrollado una plataforma que permite el registro simultáneo de los potenciales de acción de miles de células cardíacas en cultivos como respuesta al uso de un fármaco, lo cual podría agilizar considerablemente el proceso de desarrollo de fármacos y mejorar la seguridad del paciente.
Objetivo
Due to the vital role of the heart, cardiac safety assessment has become one of the major gatekeepers in all stages of the drug development process, increasing costs and resources required for new drugs. Despite the recent advancements of the Comprehensive In vitro ProArrhythmia (CiPA) initiative, there are still important obstacles related to chronic cardiotoxicities and to unreliable cell models. Thus, there is a strong need for new solutions that can improve the efficacy of drug development processes.
The MAREP project aims at tackling this problem by demonstrating a novel automated system for the comprehensive pharmacologic investigation of drug effects on in-vitro cardiac cell cultures. The novel approach combines laser excitation of novel metamaterials with high density multielectrode arrays (HD-MEA), providing high quality and high throughput recording of cardiac cells activity and offering an unprecedented and affordable method for the global study of drug effects on the heart. The outcome of the project is the prototype of a system that combines electrical recording of action potentials of thousands of cardiac cells with laser-based cell poration and intracellular recording. In essence, cardiac cells can be investigated in physiological conditions while drugs are administered and their effects studied in details on a large and reliable biological sample. The MAREP technology will boost the development of new drugs through the establishment of a novel, affordable, cost-effective and automated experimental procedure.
This project intends to exploit a research innovation born, though not exploited, in the parent ERC project Neuro-plasmonics.
This innovation may bring the laser-based bio-nanotechnologies developed in Neuro-plasmonics closer to commercialization
by introducing a novel interface that is fully compatible with standard CMOS technology and with large scale production.
Ámbito científico
Programa(s)
Régimen de financiación
ERC-POC-LS - ERC Proof of Concept Lump Sum PilotInstitución de acogida
16163 Genova
Italia