Description du projet
Récréer l’écosystème tumoral d’un patient avec l’impression 3D favorisera le traitement de précision
L’un des principaux problèmes des expériences avec des modèles est la pertinence du modèle à représenter la maladie ou l’affection humaine à évaluer. Dans le cas du cancer, il est devenu évident que l’interaction des cellules tumorales avec leur microenvironnement est essentielle à l’évolution de la maladie, à sa métastase et à la réponse à son traitement. 3DCanPredict développe un système analytique innovant utilisant un modèle tumoral bio-imprimé en 3D exploitant des échafaudages en hydrogel et les cellules tumorales du patient même. Grâce à des vaisseaux sanguins fonctionnels pouvant être imprégnés avec le sérum du patient via une pompe, le système non seulement imite le microenvironnement tumoral, mais ouvre la voie à une médecine personnalisée contre le cancer.
Objectif
Predicting clinical response to novel and existing anticancer drugs remains a major hurdle for successful cancer treatment. Studies indicate that the tumor ecosystem, resembling an organ-like structure, can limit the predictive power of current therapies that were evaluated solely on tumor cells. The interactions of tumor cells with their adjacent microenvironment are required to promote tumor progression and metastasis, determining drug responsiveness. Such interactions do not form in standard research techniques, where cancer cells grow on 2D plastic dishes. Hence, there is a need to develop new cancer models that better mimic the physio-pathological conditions of tumors. Here, we create 3D-bioprinted tumor models based on a library of hydrogels we developed as scaffold for different tumor types, designed according to the mechanical properties of the tissue of origin. As PoC, we bioprinted a vascularized 3D brain tumor model from brain tumor cells co-cultured with stromal cells and mixed with our hydrogels, that resemble the biophysics of the tumor and its microenvironment. Our patient-derived models consist of cells from a biopsy, constructed according to CT/MRI scans, and include functional vessels allowing for patients' serum to flow when connected to a pump. These models will facilitate reproducible, reliable and rapid results, determining which treatment suits best the specific patient's tumor. Taken together, this 3D-printed model could be the basis for potentially replacing cell and animal models. We predict that this powerful platform will be used in translational research for preclinical evaluation of new therapies and for clinical drug screening, which will save critical time, reduce toxicity and significantly decrease costs generating a major societal benefit. Our platform offers a highly attractive business case, as pharmaceutical and biotech companies heavily invest in preclinical predictive tools for novel personalized drug screening strategies.
Champ scientifique
Programme(s)
Régime de financement
ERC-POC-LS - ERC Proof of Concept Lump Sum PilotInstitution d’accueil
69978 Tel Aviv
Israël