Projektbeschreibung
3D-Biodruck von Tumorökosystemen fördert Präzisionsmedizin
Eine der größten Hürden experimenteller Modellsysteme ist, dass die zu analysierende menschliche Krankheit genau repräsentiert werden muss. Bei Tumoren ist inzwischen bekannt, dass Progression, Metastasierung und therapeutisches Ansprechen wesentlich von Wechselwirkungen zwischen Tumorzellen und ihrer Mikroumgebung abhängen. Das Projekt 3DCanPredict entwickelt ein neues Analysesystem, das in 3D-Biodruck ein Tumormodell aus Hydrogel-Scaffolds und patienteneigenen Tumorzellen erzeugt. Mit funktionellen Blutgefäßen, die über eine Pumpe mit dem Patientenserum infundiert werden, simuliert das System die Mikroumgebung des Tumors und ebnet damit den Weg für personalisierte Krebstherapien.
Ziel
Predicting clinical response to novel and existing anticancer drugs remains a major hurdle for successful cancer treatment. Studies indicate that the tumor ecosystem, resembling an organ-like structure, can limit the predictive power of current therapies that were evaluated solely on tumor cells. The interactions of tumor cells with their adjacent microenvironment are required to promote tumor progression and metastasis, determining drug responsiveness. Such interactions do not form in standard research techniques, where cancer cells grow on 2D plastic dishes. Hence, there is a need to develop new cancer models that better mimic the physio-pathological conditions of tumors. Here, we create 3D-bioprinted tumor models based on a library of hydrogels we developed as scaffold for different tumor types, designed according to the mechanical properties of the tissue of origin. As PoC, we bioprinted a vascularized 3D brain tumor model from brain tumor cells co-cultured with stromal cells and mixed with our hydrogels, that resemble the biophysics of the tumor and its microenvironment. Our patient-derived models consist of cells from a biopsy, constructed according to CT/MRI scans, and include functional vessels allowing for patients' serum to flow when connected to a pump. These models will facilitate reproducible, reliable and rapid results, determining which treatment suits best the specific patient's tumor. Taken together, this 3D-printed model could be the basis for potentially replacing cell and animal models. We predict that this powerful platform will be used in translational research for preclinical evaluation of new therapies and for clinical drug screening, which will save critical time, reduce toxicity and significantly decrease costs generating a major societal benefit. Our platform offers a highly attractive business case, as pharmaceutical and biotech companies heavily invest in preclinical predictive tools for novel personalized drug screening strategies.
Wissenschaftliches Gebiet
Programm/Programme
Thema/Themen
Finanzierungsplan
ERC-POC-LS - ERC Proof of Concept Lump Sum PilotGastgebende Einrichtung
69978 Tel Aviv
Israel