In-vivo Gene Editing by NanotransducErs

Información del proyecto

I-GENE

Identificador del acuerdo de subvención: 862714

DOI

10.3030/862714

Proyecto cerrado

Fecha de la firma de la CE 30 Agosto 2019

Fecha de inicio 1 Noviembre 2019

Fecha de finalización 30 Abril 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 994 878,75

Aportación de la UE

€ 2 994 878,75

Coordinado por

UNIVERSITA DI PISA
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Websites, patent fillings, videos etc. (6)

I-GENE science festival
Opening the virtual research environment (VRE)

Opening VRE to users upon subscription restricted access

Training kit

I-GENE training kit

Web-site

Launching project web-site, newsletter, brochure

Training courses 2nd Edition

IGENE training courses

Training courses

I-GENE training courses

Documents, reports (10)

Ethics -WP3 related

Ethics. Putting in place harmonised procedures for risk assessment and prevention

DECO plan 2.0

Dissemination exploitation communication and outreach DECO plan

Ethics - WP4 related

Ethical management plan to avoid misuse in research ethical authorization

Safety test results

Safety of laser radiation. The NOAL will be provided by testing the device for laser radiation

Data Management Plan

The Data Management Plan (DMP) describes the types of research data that will be generated or collected during the project and standards for datasets preservation, sharing, verification, use or reuse.

DECO plan 1.0

Dissemination, exploitation, communication and outreach (DECO) plan

NT optimization

In silico evaluation of opticalthermal behaviour and definition of the optimized NTs

NT simulation

Numerical simulation of the NT proprieties

Ethical code

IGENE ethical code

Project handbook

Operational manual that describes all the processes and procedure set for the project to ensure the highest quality standards are m

Demonstrators, pilots, prototypes (2)

Final conference

I-GENE technology showcase, final confence

Cell detection in LoC

Detection of cells flowing in the LoC (LIONIX)

Other (1)

I-GENE software

Use of predictive algorithms to optimize the g-RNA design for maximizing the on-target activity and reducing off-target effects

Publicaciones

Peer reviewed articles (7)

CRISPR/Cas9 in the era of nanomedicine and synthetic biology

Autores: Tiziana Julia NadjeschdaSchmidtBarbaraBerarducciSoultanaKonstantinidouVittoriaRaffa
Publicado en: Drug Discovery Today, Edición 13596446, 2022, ISSN 1359-6446
Editor: Elsevier BV
DOI: 10.1016/j.drudis.2022.103375

Mouse Mammary Tumor Virus (MMTV) and MMTV-like Viruses: An In-depth Look at a Controversial Issue

Autores: Francesca Parisi; Giulia Freer; Chiara Maria Mazzanti; Mauro Pistello; Alessandro Poli
Publicado en: Viruses, Edición Vol 14, Edición 5, 2022, Página(s) 977, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14050977

Ablation of Acid Ceramidase Impairs Autophagy and Mitochondria Activity in Melanoma Cells

Autores: Michele Lai, Veronica La Rocca, Rachele Amato, Giulia Freer, Mario Costa, Pietro Giorgio Spezia, Paola Quaranta, Giuseppe Lombardo, Daniele Piomelli, Mauro Pistello
Publicado en: International Journal of Molecular Sciences, Edición 22/6, 2021, Página(s) 3247, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22063247

Effects of Metal Oxide Nanoparticles in Zebrafish

Autores: Marta d’Amora; Tiziana Julia Nadjeschda Schmidt; Soultana Konstantinidou; Vittoria Raffa; Francesco De Angelis; Francesco Tantussi
Publicado en: Oxidative Medicine and Cellular Longevity, Edición Vol 2022, 2022, Página(s) 3313016, ISSN 1942-0900
Editor: Landes Bioscience
DOI: 10.1155/2022/3313016

Palmitoylethanolamide (PEA) Inhibits SARS-CoV-2 Entry by Interacting with S Protein and ACE-2 Receptor

Autores: Rossella Fonnesu; Venkata Bala Sai Chaitanya Thunuguntla; Ganesh Kumar Veeramachaneni; Jayakumar Singh Bondili; Veronica La Rocca; Carolina Filipponi; Pietro Giorgio Spezia; Maria Sidoti; Erika Plicanti; Paola Quaranta; Giulia Freer; Mauro Pistello; Michael Lee Mathai; Michele Lai
Publicado en: Viruses, Edición Volume 14; Edición 5, 2022, Página(s) 1080, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14051080

Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin

Autores: Michele Lai, Rachele Amato, Veronica La Rocca, Mesut Bilgin, Giulia Freer, Piergiorgio Spezia, Paola Quaranta, Daniele Piomelli, Mauro Pistello
Publicado en: Scientific Reports, Edición 11/1, 2021, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-90219-1

Toxicological Profile of Plasmonic Nanoparticles in Zebrafish Model

Autores: Marta d’Amora, Vittoria Raffa, Francesco De Angelis, Francesco Tantussi
Publicado en: International Journal of Molecular Sciences, Edición 22/12, 2021, Página(s) 6372, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126372

Non-peer reviewed articles (3)

A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations

Autores: Michele Lai, Elena Iacono, Pietro Giorgio Spezia, Giulia Lottini, Veronica La Rocca, PaolaQuaranta, Mauro Pistello, Giulia Freer
Publicado en: Journal of Virological Methods, Edición Volume 299, January 2022,, 2022, Página(s) 114327, ISSN 0166-0934
Editor: Elsevier BV
DOI: 10.1016/j.jviromet.2021.114327

CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats

Autores: Michele Lai, Eyal Maori, Paola Quaranta, Giulia Matteoli, Fabrizio Maggi, Marco Sgarbanti, Stefania Crucitta, , Simone Pacini, Ombretta Turriziani, Guido Antonelli, Jonathan L. Heeney, Giulia Freer, Mauro Pistello
Publicado en: Journal of Virology, Edición Vol. 95, No. 23, 2021, Página(s) No. 23, ISSN 2150-7511
Editor: American Society for Microbiology
DOI: 10.1128/jvi.01358-21

A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 protein

Autores: Soultana Konstantinidou, Agnieszka Lindstaedt, Tiziana Julia Nadjeschda Schmidt, Francesco Nocilla, Giovanni Maltinti, Mafalda Angelica Rocco, Elena Landi, Alessandro De Carli, Stefania Crucitta, Michele Lai, Mauro Pistello, Valentina Cappello, Dariusz Witt, Chiara Gabellini, Piotr Barski, Vittoria Raffa
Publicado en: BioRxiv, 2024, ISSN 2692-8205
Editor: BioXriv
DOI: 10.1101/2024.07.09.602746

Other (2)

A photo-switchable gold nanoformulation based on the dCas9 protein for spatiotemporal controlled gene editing activation in vivo

Autores: Soultana Konstantinidou, Mafalda A. Rocco, Francesco Nocilla, Doriana Debellis, Marta d’Amora, Francesco De Angelis, Chiara Gabellini, Francesco Fuso, Francesco Tantussi, Vittoria Raffa
Publicado en: bioRxiv, 2024, Página(s) 598872, ISSN 2692-8205
Editor: bioRxiv
DOI: 10.1101/2024.06.13.598872

N-acylethanolamine acid amide hydrolase is a novel target for drugs against SARS-CoV-2 and Zika virus

Autores: Michele Lai; Veronica La Rocca; Rachele Amato; Elena Iacono; Carolina Filipponi; Elisa Catelli; Lucia Bogani; Rossella Fonnesu; Giulia Lottini; Alessandro De Carli; Alessandro Mengozzi; Stefano Masi; Paola Quaranta; Pietro Giorgio Spezia; Giulia Freer; Paola Lenzi; Francesco Fornai; Daniele Piomelli; Mauro Pistello
Publicado en: BiorXiv, 2022, ISSN 2692-8205
Editor: BiorXiv
DOI: 10.1101/2022.02.08.479661

Derechos de propiedad intelectual

Patent (2)

NANOTRANSDUCER-BASED GENOME EDITING SYSTEM

Número de solicitud/publicación: 20 20050432
Fecha: 2020-01-21
Solicitante(s): FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

NANOTRANSDUCER-BASED GENOME EDITING SYSTEM

Número de solicitud/publicación: 20 20050432
Fecha: 2020-01-21
Solicitante(s): UNIVERSITA DI PISA