In-vivo Gene Editing by NanotransducErs

Informations projet

I-GENE

N° de convention de subvention: 862714

DOI

10.3030/862714

Projet clôturé

Date de signature de la CE 30 Août 2019

Date de début 1 Novembre 2019

Date de fin 30 Avril 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 994 878,75

Contribution de l’UE

€ 2 994 878,75

Coordonné par

UNIVERSITA DI PISA
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Websites, patent fillings, videos etc. (6)

I-GENE science festival
Opening the virtual research environment (VRE)

Opening VRE to users upon subscription restricted access

Training kit

I-GENE training kit

Web-site

Launching project web-site, newsletter, brochure

Training courses 2nd Edition

IGENE training courses

Training courses

I-GENE training courses

Documents, reports (10)

Ethics -WP3 related

Ethics. Putting in place harmonised procedures for risk assessment and prevention

DECO plan 2.0

Dissemination exploitation communication and outreach DECO plan

Ethics - WP4 related

Ethical management plan to avoid misuse in research ethical authorization

Safety test results

Safety of laser radiation. The NOAL will be provided by testing the device for laser radiation

Data Management Plan

The Data Management Plan (DMP) describes the types of research data that will be generated or collected during the project and standards for datasets preservation, sharing, verification, use or reuse.

DECO plan 1.0

Dissemination, exploitation, communication and outreach (DECO) plan

NT optimization

In silico evaluation of opticalthermal behaviour and definition of the optimized NTs

NT simulation

Numerical simulation of the NT proprieties

Ethical code

IGENE ethical code

Project handbook

Operational manual that describes all the processes and procedure set for the project to ensure the highest quality standards are m

Demonstrators, pilots, prototypes (2)

Final conference

I-GENE technology showcase, final confence

Cell detection in LoC

Detection of cells flowing in the LoC (LIONIX)

Other (1)

I-GENE software

Use of predictive algorithms to optimize the g-RNA design for maximizing the on-target activity and reducing off-target effects

Publications

Peer reviewed articles (7)

CRISPR/Cas9 in the era of nanomedicine and synthetic biology

Auteurs: Tiziana Julia NadjeschdaSchmidtBarbaraBerarducciSoultanaKonstantinidouVittoriaRaffa
Publié dans: Drug Discovery Today, Numéro 13596446, 2022, ISSN 1359-6446
Éditeur: Elsevier BV
DOI: 10.1016/j.drudis.2022.103375

Mouse Mammary Tumor Virus (MMTV) and MMTV-like Viruses: An In-depth Look at a Controversial Issue

Auteurs: Francesca Parisi; Giulia Freer; Chiara Maria Mazzanti; Mauro Pistello; Alessandro Poli
Publié dans: Viruses, Numéro Vol 14, Numéro 5, 2022, Page(s) 977, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14050977

Ablation of Acid Ceramidase Impairs Autophagy and Mitochondria Activity in Melanoma Cells

Auteurs: Michele Lai, Veronica La Rocca, Rachele Amato, Giulia Freer, Mario Costa, Pietro Giorgio Spezia, Paola Quaranta, Giuseppe Lombardo, Daniele Piomelli, Mauro Pistello
Publié dans: International Journal of Molecular Sciences, Numéro 22/6, 2021, Page(s) 3247, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22063247

Effects of Metal Oxide Nanoparticles in Zebrafish

Auteurs: Marta d’Amora; Tiziana Julia Nadjeschda Schmidt; Soultana Konstantinidou; Vittoria Raffa; Francesco De Angelis; Francesco Tantussi
Publié dans: Oxidative Medicine and Cellular Longevity, Numéro Vol 2022, 2022, Page(s) 3313016, ISSN 1942-0900
Éditeur: Landes Bioscience
DOI: 10.1155/2022/3313016

Palmitoylethanolamide (PEA) Inhibits SARS-CoV-2 Entry by Interacting with S Protein and ACE-2 Receptor

Auteurs: Rossella Fonnesu; Venkata Bala Sai Chaitanya Thunuguntla; Ganesh Kumar Veeramachaneni; Jayakumar Singh Bondili; Veronica La Rocca; Carolina Filipponi; Pietro Giorgio Spezia; Maria Sidoti; Erika Plicanti; Paola Quaranta; Giulia Freer; Mauro Pistello; Michael Lee Mathai; Michele Lai
Publié dans: Viruses, Numéro Volume 14; Numéro 5, 2022, Page(s) 1080, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14051080

Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin

Auteurs: Michele Lai, Rachele Amato, Veronica La Rocca, Mesut Bilgin, Giulia Freer, Piergiorgio Spezia, Paola Quaranta, Daniele Piomelli, Mauro Pistello
Publié dans: Scientific Reports, Numéro 11/1, 2021, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-021-90219-1

Toxicological Profile of Plasmonic Nanoparticles in Zebrafish Model

Auteurs: Marta d’Amora, Vittoria Raffa, Francesco De Angelis, Francesco Tantussi
Publié dans: International Journal of Molecular Sciences, Numéro 22/12, 2021, Page(s) 6372, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126372

Non-peer reviewed articles (3)

A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations

Auteurs: Michele Lai, Elena Iacono, Pietro Giorgio Spezia, Giulia Lottini, Veronica La Rocca, PaolaQuaranta, Mauro Pistello, Giulia Freer
Publié dans: Journal of Virological Methods, Numéro Volume 299, January 2022,, 2022, Page(s) 114327, ISSN 0166-0934
Éditeur: Elsevier BV
DOI: 10.1016/j.jviromet.2021.114327

CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats

Auteurs: Michele Lai, Eyal Maori, Paola Quaranta, Giulia Matteoli, Fabrizio Maggi, Marco Sgarbanti, Stefania Crucitta, , Simone Pacini, Ombretta Turriziani, Guido Antonelli, Jonathan L. Heeney, Giulia Freer, Mauro Pistello
Publié dans: Journal of Virology, Numéro Vol. 95, No. 23, 2021, Page(s) No. 23, ISSN 2150-7511
Éditeur: American Society for Microbiology
DOI: 10.1128/jvi.01358-21

A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 protein

Auteurs: Soultana Konstantinidou, Agnieszka Lindstaedt, Tiziana Julia Nadjeschda Schmidt, Francesco Nocilla, Giovanni Maltinti, Mafalda Angelica Rocco, Elena Landi, Alessandro De Carli, Stefania Crucitta, Michele Lai, Mauro Pistello, Valentina Cappello, Dariusz Witt, Chiara Gabellini, Piotr Barski, Vittoria Raffa
Publié dans: BioRxiv, 2024, ISSN 2692-8205
Éditeur: BioXriv
DOI: 10.1101/2024.07.09.602746

Other (2)

A photo-switchable gold nanoformulation based on the dCas9 protein for spatiotemporal controlled gene editing activation in vivo

Auteurs: Soultana Konstantinidou, Mafalda A. Rocco, Francesco Nocilla, Doriana Debellis, Marta d’Amora, Francesco De Angelis, Chiara Gabellini, Francesco Fuso, Francesco Tantussi, Vittoria Raffa
Publié dans: bioRxiv, 2024, Page(s) 598872, ISSN 2692-8205
Éditeur: bioRxiv
DOI: 10.1101/2024.06.13.598872

N-acylethanolamine acid amide hydrolase is a novel target for drugs against SARS-CoV-2 and Zika virus

Auteurs: Michele Lai; Veronica La Rocca; Rachele Amato; Elena Iacono; Carolina Filipponi; Elisa Catelli; Lucia Bogani; Rossella Fonnesu; Giulia Lottini; Alessandro De Carli; Alessandro Mengozzi; Stefano Masi; Paola Quaranta; Pietro Giorgio Spezia; Giulia Freer; Paola Lenzi; Francesco Fornai; Daniele Piomelli; Mauro Pistello
Publié dans: BiorXiv, 2022, ISSN 2692-8205
Éditeur: BiorXiv
DOI: 10.1101/2022.02.08.479661

Droits de propriété intellectuelle

Patent (2)

NANOTRANSDUCER-BASED GENOME EDITING SYSTEM

Numéro de demande/publication: 20 20050432
Date: 2020-01-21
Demandeur(s): FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA

NANOTRANSDUCER-BASED GENOME EDITING SYSTEM

Numéro de demande/publication: 20 20050432
Date: 2020-01-21
Demandeur(s): UNIVERSITA DI PISA