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Towards an instrument-free future of molecular diagnostics at the point-of-care

Deliverables

Report on the design and synthesis of constructs and oligos

Three plasmids containing the three targets will be prepared and the specific primers and probes will be developed

Dissemination and staff exchange report for Year 1

Report on publications to the scientific community conferences and the general public and list of staff exchange visits at Year 1

Publications

Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Author(s): Serafina Serena Amoia; Angelantonio Minafra; Angela Ligorio; Vincenzo Cavalieri; Donato Boscia; Maria Saponari; Giuliana Loconsole
Published in: Agriculture, Issue 13, 2023, Page(s) 716, ISSN 2077-0472
Publisher: MDPI
DOI: 10.3390/agriculture13030716

Naked-Eye Detection of LAMP-Produced Nucleic Acids in Saliva using Chitosan-capped AuNPs in a Single-Tube Assay

Author(s): Stylianos Grammatikos; Ioannis Svoliantopoulos; Electra Gizeli
Published in: Analytical Chemistry, Issue 95, 50,, 2023, Page(s) 18514–18521, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1101/2023.06.09.23291198

Real-time colorimetric LAMP methodology for quantitative colorimetric nucleic acids detection at the point-of-care

Author(s): G. Papadakis, A.K. Pantazis, N. Fikas, S. Chatziioannidou, V. Tsiakalou, V. Pogka, M. Megariti, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli
Published in: Scientific Reports, Issue 12, 2022, Page(s) Article Number: 3775, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-06632-7

A Colorimetric LAMP Detection of Xylella fastidiosa in Crude Alkaline Sap of Olive Trees in Apulia as a Field-Based Tool for Disease Containment

Author(s): Serafina Serena Amoia; Giuliana Loconsole; Angela Ligorio; Alexandros K. Pantazis; George Papadakis; Electra Gizeli; Angelantonio Minafra
Published in: Volume 13, Issue 4, 2023, Page(s) 448, ISSN 2077-0472
Publisher: MDPI
DOI: 10.3390/agriculture13020448

JDLL: A library to run Deep Learning models on Java bioimage informatics platforms

Author(s): de Haro, Carlos Garcia Lopez; Dallongeville, Stephane; Musset, Thomas; de Mariscal, Estibaliz Gomez; Sage, Daniel; Ouyang, Wei; Munoz-Barrutia, Arrate; Tinevez, Jean-Yves; Olivo-Marin, Jean-Christophe
Published in: Nature Methods, Issue 5, 2024, ISSN 1476-4687
Publisher: Nature
DOI: 10.48550/arxiv.2306.04796

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