Towards an instrument-free future of molecular diagnostics at the point-of-care

Project Information

FreeATPOC

Grant agreement ID: 862840

DOI

10.3030/862840

Project closed

EC signature date 30 August 2019

Start date 1 January 2020

End date 31 December 2023

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 3 052 500,00

EU contribution

€ 3 052 500,00

Coordinated by

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (12)

Report on the design and synthesis of constructs and oligos

Three plasmids containing the three targets will be prepared and the specific primers and probes will be developed

Optimized conditions for influenza and HIV assay in patients' samples

Optimization of the influenza and HIV developed kits for detection in patients samples

Extensive lab-validation of detection procedure applied to X. fastidiosa artificial hosts

Optimization of the kit developed for Xylella; extensive lab-validation of detection procedure applied to artificial hosts

Dissemination and staff exchange report for Year 1

Report on publications to the scientific community conferences and the general public and list of staff exchange visits at Year 1

Optimized protocol for targets amplification in purified samples

Optimized protocol for targets amplification in purified samples and at a non-optimal temperature range

Dissemination and staff exchange report for Year 2

Report on publications to the scientific community, conferences and the general public and list of staff exchange visits for Year 2

Report on design/production of 3D printed cartridges

Design/production of 3D printed cartridges; report on methodology and implementation

Optimized protocols for sample pretreatment & NA amplification

Optimization of sample pretreatment protocol and NA amplification in crude samples

Results on field test application of developed kits in naturally infected olive groves and ornamentals (

Field trials for the detection of Xylella; application of developed kits in naturally infected olive groves and ornamentals

Evaluation/Proof of concept report for HIV assay

Evaluation of HIV NA detection;proof of concept report for HIV assay

Evaluation/proof-of-concept report for influenza RNA detection assay

Evaluation of influenza kit in a hospital setting; proof-of-concept for influenza RNA detection assay

Dissemination and staff exchange report for Year 3

Report on publications to the scientific community, conferences and the general public and list of staff exchange visits for Year 3

Other (2)

Organization of international conference/workshop

Organization of at least one international conference or workshop on molecular diagnostics aiming to present the project results to stakeholders and end users

Molecular kits for all targets

Molecular kits for all targets , i.e. influenza, HIV and Xylella

Websites, patent fillings, videos etc. (1)

Website construction

Publications

Peer reviewed articles (5)

Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Author(s): Serafina Serena Amoia; Angelantonio Minafra; Angela Ligorio; Vincenzo Cavalieri; Donato Boscia; Maria Saponari; Giuliana Loconsole
Published in: Agriculture, Issue 13, 2023, Page(s) 716, ISSN 2077-0472
Publisher: MDPI
DOI: 10.3390/agriculture13030716

Naked-Eye Detection of LAMP-Produced Nucleic Acids in Saliva using Chitosan-capped AuNPs in a Single-Tube Assay

Author(s): Stylianos Grammatikos; Ioannis Svoliantopoulos; Electra Gizeli
Published in: Analytical Chemistry, Issue 95, 50,, 2023, Page(s) 18514–18521, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1101/2023.06.09.23291198

Real-time colorimetric LAMP methodology for quantitative colorimetric nucleic acids detection at the point-of-care

Author(s): G. Papadakis, A.K. Pantazis, N. Fikas, S. Chatziioannidou, V. Tsiakalou, V. Pogka, M. Megariti, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli
Published in: Scientific Reports, Issue 12, 2022, Page(s) Article Number: 3775, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-06632-7

A Colorimetric LAMP Detection of Xylella fastidiosa in Crude Alkaline Sap of Olive Trees in Apulia as a Field-Based Tool for Disease Containment

Author(s): Serafina Serena Amoia; Giuliana Loconsole; Angela Ligorio; Alexandros K. Pantazis; George Papadakis; Electra Gizeli; Angelantonio Minafra
Published in: Volume 13, Issue 4, 2023, Page(s) 448, ISSN 2077-0472
Publisher: MDPI
DOI: 10.3390/agriculture13020448

JDLL: A library to run Deep Learning models on Java bioimage informatics platforms

Author(s): de Haro, Carlos Garcia Lopez; Dallongeville, Stephane; Musset, Thomas; de Mariscal, Estibaliz Gomez; Sage, Daniel; Ouyang, Wei; Munoz-Barrutia, Arrate; Tinevez, Jean-Yves; Olivo-Marin, Jean-Christophe
Published in: Nature Methods, Issue 5, 2024, ISSN 1476-4687
Publisher: Nature
DOI: 10.48550/arxiv.2306.04796