Towards an instrument-free future of molecular diagnostics at the point-of-care

Informazioni relative al progetto

FreeATPOC

ID dell’accordo di sovvenzione: 862840

DOI

10.3030/862840

Progetto chiuso

Data della firma CE 30 Agosto 2019

Data di avvio 1 Gennaio 2020

Data di completamento 31 Dicembre 2023

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 052 500,00

Contributo UE

€ 3 052 500,00

Coordinato da

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (12)

Report on the design and synthesis of constructs and oligos

Three plasmids containing the three targets will be prepared and the specific primers and probes will be developed

Optimized conditions for influenza and HIV assay in patients' samples

Optimization of the influenza and HIV developed kits for detection in patients samples

Extensive lab-validation of detection procedure applied to X. fastidiosa artificial hosts

Optimization of the kit developed for Xylella; extensive lab-validation of detection procedure applied to artificial hosts

Dissemination and staff exchange report for Year 1

Report on publications to the scientific community conferences and the general public and list of staff exchange visits at Year 1

Optimized protocol for targets amplification in purified samples

Optimized protocol for targets amplification in purified samples and at a non-optimal temperature range

Dissemination and staff exchange report for Year 2

Report on publications to the scientific community, conferences and the general public and list of staff exchange visits for Year 2

Report on design/production of 3D printed cartridges

Design/production of 3D printed cartridges; report on methodology and implementation

Optimized protocols for sample pretreatment & NA amplification

Optimization of sample pretreatment protocol and NA amplification in crude samples

Results on field test application of developed kits in naturally infected olive groves and ornamentals (

Field trials for the detection of Xylella; application of developed kits in naturally infected olive groves and ornamentals

Evaluation/Proof of concept report for HIV assay

Evaluation of HIV NA detection;proof of concept report for HIV assay

Evaluation/proof-of-concept report for influenza RNA detection assay

Evaluation of influenza kit in a hospital setting; proof-of-concept for influenza RNA detection assay

Dissemination and staff exchange report for Year 3

Report on publications to the scientific community, conferences and the general public and list of staff exchange visits for Year 3

Other (2)

Organization of international conference/workshop

Organization of at least one international conference or workshop on molecular diagnostics aiming to present the project results to stakeholders and end users

Molecular kits for all targets

Molecular kits for all targets , i.e. influenza, HIV and Xylella

Websites, patent fillings, videos etc. (1)

Website construction

Pubblicazioni

Peer reviewed articles (5)

Detection of Xylella fastidiosa in Host Plants and Insect Vectors by Droplet Digital PCR

Autori: Serafina Serena Amoia; Angelantonio Minafra; Angela Ligorio; Vincenzo Cavalieri; Donato Boscia; Maria Saponari; Giuliana Loconsole
Pubblicato in: Agriculture, Numero 13, 2023, Pagina/e 716, ISSN 2077-0472
Editore: MDPI
DOI: 10.3390/agriculture13030716

Naked-Eye Detection of LAMP-Produced Nucleic Acids in Saliva using Chitosan-capped AuNPs in a Single-Tube Assay

Autori: Stylianos Grammatikos; Ioannis Svoliantopoulos; Electra Gizeli
Pubblicato in: Analytical Chemistry, Numero 95, 50,, 2023, Pagina/e 18514–18521, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1101/2023.06.09.23291198

Real-time colorimetric LAMP methodology for quantitative colorimetric nucleic acids detection at the point-of-care

Autori: G. Papadakis, A.K. Pantazis, N. Fikas, S. Chatziioannidou, V. Tsiakalou, V. Pogka, M. Megariti, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli
Pubblicato in: Scientific Reports, Numero 12, 2022, Pagina/e Article Number: 3775, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-06632-7

A Colorimetric LAMP Detection of Xylella fastidiosa in Crude Alkaline Sap of Olive Trees in Apulia as a Field-Based Tool for Disease Containment

Autori: Serafina Serena Amoia; Giuliana Loconsole; Angela Ligorio; Alexandros K. Pantazis; George Papadakis; Electra Gizeli; Angelantonio Minafra
Pubblicato in: Volume 13, Numero 4, 2023, Pagina/e 448, ISSN 2077-0472
Editore: MDPI
DOI: 10.3390/agriculture13020448

JDLL: A library to run Deep Learning models on Java bioimage informatics platforms

Autori: de Haro, Carlos Garcia Lopez; Dallongeville, Stephane; Musset, Thomas; de Mariscal, Estibaliz Gomez; Sage, Daniel; Ouyang, Wei; Munoz-Barrutia, Arrate; Tinevez, Jean-Yves; Olivo-Marin, Jean-Christophe
Pubblicato in: Nature Methods, Numero 5, 2024, ISSN 1476-4687
Editore: Nature
DOI: 10.48550/arxiv.2306.04796