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MIXed plastics biodegradation and UPcycling using microbial communities

Deliverables

Final Data Management Plan

Related to task 82Final Data Management Plan

Initial Data Management Plan

Related to task 8.2: Initial Data Management Plan

Online blog as platform for consistent public dissemination

Related to task 8.1: An online blog as platform for consistent public dissemination. After setting up the blog, frequent posts (English, minimum four per month) will report about the project’s progress and news from the global plastic crisis to set the consortium’s work in a comprehensive context. The blog will be linked to the social media channels of the consortium members to maximize the communication and impact (PGS).

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Publications

Synthetic Biology and Genome-Editing Tools for Improving PHA Metabolic Engineering

Author(s): Xu Zhang, Yina Lin, Qiong Wu, Ying Wang, Guo-Qiang Chen
Published in: Trends in Biotechnology, 38/7, 2020, Page(s) 689-700, ISSN 0167-7799
Publisher: Elsevier BV
DOI: 10.1016/j.tibtech.2019.10.006

Enzymatic degradation of polyethylene terephthalate nanoplastics analyzed in real time by isothermal titration calorimetry

Author(s): Kristina Vogel, Ren Wei, Lara Pfaff, Daniel Breite, Hassan Al-Fathi, Christian Ortmann, Irina Estrela-Lopis, Tom Venus, Agnes Schulze, Hauke Harms, Uwe T. Bornscheuer, Thomas Maskow
Published in: Science of The Total Environment, 773, 2021, Page(s) 145111, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2021.145111

Anti-staphylococcal hydrogels based on bacterial cellulose and the antimicrobial biopolyester poly(3-hydroxy-acetylthioalkanoate-co-3-hydroxyalkanoate)

Author(s): Virginia Rivero-Buceta, María Rosa Aguilar, Ana María Hernández-Arriaga, Francisco G. Blanco, Antonia Rojas, Marta Tortajada, Rosa Ana Ramírez-Jiménez, Blanca Vázquez-Lasa, Auxiliadora Prieto
Published in: International Journal of Biological Macromolecules, 162, 2020, Page(s) 1869-1879, ISSN 0141-8130
Publisher: Elsevier BV
DOI: 10.1016/j.ijbiomac.2020.07.289

Chemical and biological catalysis for plastics recycling and upcycling

Author(s): Lucas D. Ellis, Nicholas A. Rorrer, Kevin P. Sullivan, Maike Otto, John E. McGeehan, Yuriy Román-Leshkov, Nick Wierckx, Gregg T. Beckham
Published in: Nature Catalysis, 4/7, 2021, Page(s) 539-556, ISSN 2520-1158
Publisher: Springer Nature
DOI: 10.1038/s41929-021-00648-4

MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020

Author(s): Hendrik Ballerstedt, Till Tiso, Nick Wierckx, Ren Wei, Luc Averous, Uwe Bornscheuer, Kevin O’Connor, Tilman Floehr, Andreas Jupke, Jürgen Klankermayer, Luo Liu, Victor de Lorenzo, Tanja Narancic, Juan Nogales, Rémi Perrin, Eric Pollet, Auxiliadora Prieto, William Casey, Thomas Haarmann, Alexandru Sarbu, Ulrich Schwaneberg, Fengxue Xin, Weiliang Dong, Jiamin Xing, Guo-Qiang Chen, Tianwei Tan, M
Published in: Environmental Sciences Europe, 33/1, 2021, Page(s) 99, ISSN 2190-4707
Publisher: Springer Verlag
DOI: 10.1186/s12302-021-00536-5

High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Author(s): Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Published in: iScience, 23/3, 2020, Page(s) 100946, ISSN 2589-0042
Publisher: Elsevier
DOI: 10.1016/j.isci.2020.100946

Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis

Author(s): Lin-Ping Yu, Xu Yan, Xu Zhang, Xiang-Bin Chen, Qiong Wu, Xiao-Ran Jiang, Guo-Qiang Chen
Published in: Metabolic Engineering, 59, 2020, Page(s) 119-130, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2020.02.005

Polyhydroxyalkanoates (PHA) toward cost competitiveness and functionality

Author(s): Guo-Qiang Chen, Xin-Yu Chen, Fu-Qing Wu, Jin-Chun Chen
Published in: Advanced Industrial and Engineering Polymer Research, 3/1, 2020, Page(s) 1-7, ISSN 2542-5048
Publisher: Science Direct
DOI: 10.1016/j.aiepr.2019.11.001

A pseudo-homogeneous system for PET glycolysis using a colloidal catalyst of graphite carbon nitride in ethylene glycol

Author(s): Zishuai Wang, Yaoqiang Wang, Shengnan Xu, Yu Jin, Zequn Tang, Gang Xiao, Haijia Su
Published in: Polymer Degradation and Stability, 190, 2021, Page(s) 109638, ISSN 0141-3910
Publisher: Elsevier BV
DOI: 10.1016/j.polymdegradstab.2021.109638

Engineering Halomonas bluephagenesis for L-Threonine production

Author(s): Hetong Du, Yiqing Zhao, Fuqing Wu, Peifei Ouyang, Jinchun Chen, Xiaoran Jiang, Jianwen Ye, Guo-Qiang Chen
Published in: Metabolic Engineering, 60, 2020, Page(s) 119-127, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2020.04.004

Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and Recovery.

Author(s): Christian C. Blesken; Tessa Strümpfler; Till Tiso; Lars M. Blank
Published in: Microorganisms, 2, 2020, ISSN 2078-2489
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.18154/rwth-2021-00396

For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!

Author(s): Víctor de Lorenzo, Natalio Krasnogor, Markus Schmidt
Published in: New Biotechnology, 60, 2021, Page(s) 44-51, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2020.08.004

Synthetic Control of Metabolic States in Pseudomonas putida by Tuning Polyhydroxyalkanoate Cycle

Author(s): Maria-Tsampika Manoli, Juan Nogales, Auxiliadora Prieto
Published in: mBio, January/February 2022 Volume 13 Issue 11, 2022, ISSN 2150-7511
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.01794-21

ArsH protects Pseudomonas putida from oxidative damage caused by exposure to arsenic

Author(s): A. David Páez‐Espino, Pablo I. Nikel, Max Chavarría, Víctor Lorenzo
Published in: Environmental Microbiology, 22/6, 2020, Page(s) 2230-2242, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.14991

Towards bio-upcycling of polyethylene terephthalate

Author(s): Till Tiso, Tanja Narancic, Ren Wei, Eric Pollet, Niall Beagan, Katja Schröder, Annett Honak, Mengying Jiang, Shane T. Kenny, Nick Wierckx, Rémi Perrin, Luc Avérous, Wolfgang Zimmermann, Kevin O'Connor, Lars M. Blank
Published in: Metabolic Engineering, 66, 2021, Page(s) 167-178, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.03.011

An updated structural model of the A domain of the Pseudomonas putida   XylR  regulator poses an atypical interplay with aromatic effectors

Author(s): Pavel Dvořák, Carlos Alvarez‐Carreño, Sergio Ciordia, Alberto Paradela, Víctor Lorenzo
Published in: Environmental Microbiology, 23/8, 2021, Page(s) 4418-4433, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15628

Tailor‐Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila

Author(s): Mengyi Li, Yueyuan Ma, Xu Zhang, Lizhan Zhang, Xinyu Chen, Jian‐Wen Ye, Guo‐Qiang Chen
Published in: Advanced Materials, 2021, Page(s) 2102766, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202102766

Mixed plastics biodegradation and UPcycling using microbial communities: the NSFC-EU 2019 project MIX-UP to help achieve “carbon neutrality”

Author(s): Jie Zhou, Haijia Su, Qiong Wu, Jianmin Xing, Weiliang Dong and Min Jiang
Published in: Chinese Journal of Biotechnology, 2021, ISSN 1000-3061
Publisher: Kexue Chubaneshe/Science Press
DOI: 10.13345/j.cjb.210398

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

Author(s): Pablo I. Nikel, Tobias Fuhrer, Max Chavarría, Alberto Sánchez-Pascuala, Uwe Sauer, Víctor de Lorenzo
Published in: The ISME Journal, 15/6, 2021, Page(s) 1751-1766, ISSN 1751-7362
Publisher: Nature Publishing Group
DOI: 10.1038/s41396-020-00884-9

Novel Magnetic Carbon Supported Molybdenum Disulfide Catalyst and Its Application in Residue Upgrading

Author(s): Xiangyang Zhu, Dong Qiao, Liangrong Yang, Qinling Bi, Huifang Xing, Shan Ni, Menglei Yuan, Huizhou Liu, Luhai Wang, An Ma
Published in: Green Energy & Environment, 2020, ISSN 2468-0257
Publisher: ELSEVIER ScienceDirect
DOI: 10.1016/j.gee.2020.06.025

From Residues to Added-Value Bacterial Biopolymers as Nanomaterials for Biomedical Applications

Author(s): Francisco G. Blanco, Natalia Hernández, Virginia Rivero-Buceta, Beatriz Maestro, Jesús M. Sanz, Aránzazu Mato, Ana M. Hernández-Arriaga, M. Auxiliadora Prieto
Published in: Nanomaterials, 11/6, 2021, Page(s) 1492, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano11061492

Editorial: Microbial Degradation of Plastics

Author(s): Ren Wei, Nick Wierckx
Published in: Frontiers in Microbiology, 12, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.635621

Concomitant prediction of environmental fate and toxicity of chemical compounds

Author(s): Juan Antonio Garcia-Martin, Max Chavarría, Victor de Lorenzo, Florencio Pazos
Published in: Biology Methods and Protocols, 5/1, 2020, ISSN 2396-8923
Publisher: Oxford University Press
DOI: 10.1093/biomethods/bpaa025

Process analysis of microplastic degradation using activated PMS and Fenton reagents

Author(s): Bin Liu, Qixuan Jiang, Zenghui Qiu, Luo Liu, Ren Wei, Xin Zhang, Haijun Xu
Published in: Chemosphere, 2022, ISSN 0045-6535
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.chemosphere.2022.134220

Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida

Author(s): Pavel Dvořák, Edward A. Bayer, Víctor de Lorenzo
Published in: ACS Synthetic Biology, 9/10, 2020, Page(s) 2749-2764, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.0c00276

ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces.

Author(s): Yamal Al-ramahi, Akos Nyerges, Yago Margolles, Lidia Cerdán, Gyorgyi Ferenc, Csaba Pál, Luis Ángel Fernández, Víctor de Lorenzo
Published in: Communications Biology, volume 4, Article number: 1169, 2021, ISSN 2399-3642
Publisher: Nature Research
DOI: 10.1038/s42003-021-02702-0

Fabrication of hypercrosslinked hydroxyl-rich solid phase extractants for cesium separation from the salt lake brine

Author(s): Zihao Xu, Meng Rong, Qiyu Meng, Haoyu Yao, Shan Ni, Li Wang, Huifang Xing, Hongnan Qu, Liangrong Yang, Huizhou Liu
Published in: Chemical Engineering Journal, 400, 2020, Page(s) 125991, ISSN 1385-8947
Publisher: Elsevier BV
DOI: 10.1016/j.cej.2020.125991

Possibilities and limitations of biotechnological plastic degradation and recycling

Author(s): Ren Wei, Till Tiso, Jürgen Bertling, Kevin O’Connor, Lars M. Blank, Uwe T. Bornscheuer
Published in: Nature Catalysis, Monthly journal, 2020, ISSN 2520-1158
Publisher: Springer Nature
DOI: 10.1038/s41929-020-00521-w

A Standardized Inverter Package Borne by Broad Host Range Plasmids for Genetic Circuit Design in Gram-Negative Bacteria

Author(s): Huseyin Tas, Ángel Goñi-Moreno, Víctor de Lorenzo
Published in: ACS Synthetic Biology, 10/1, 2021, Page(s) 213-217, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.0c00529

A Combined Bio-Chemical Synthesis Route for 1-Octene Sheds Light on Rhamnolipid Structure

Author(s): Till Tiso, Daniel F. Sauer, Klaus Beckerle, Christian C. Blesken, Jun Okuda, Lars M. Blank
Published in: Catalysts, 10/8, 2020, Page(s) 874, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10080874

Efficient recovery of bio-based 1,2,4-butanetriol by using boronic acid anionic reactive extraction

Author(s): Qiyu Meng, Jiemiao Yu, Liangrong Yang, Yanqing Li, Mo Xian, Huizhou Liu
Published in: Separation and Purification Technology, 255, 2021, Page(s) 117728, ISSN 1383-5866
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.seppur.2020.117728

Fusion of Chitin-Binding Domain From Chitinolyticbacter meiyuanensis SYBC-H1 to the Leaf-Branch Compost Cutinase for Enhanced PET Hydrolysis

Author(s): Rui Xue, Yinping Chen, Huan Rong, Ren Wei, Zhongli Cui, Jie Zhou, Weiliang Dong, Min Jiang
Published in: Frontiers in Bioengineering and Biotechnology, 2021, ISSN 2296-4185
Publisher: Frontiers Media S.A.
DOI: 10.3389/fbioe.2021.762854

Standardization of inducer-activated broad host range expression modules: Debugging and refactoring an alkane-responsive AlkS/PalkB device.

Author(s): Alejandro Arce-Rodríguez, Ilaria Benedetti, José Manuel Borrero-de Acuña, Rafael Silva-Rocha, Víctor de Lorenzo
Published in: Synthetic Biology, Volume 6, Issue 1, 2021, Page(s) 1–11, ISSN 2397-7000
Publisher: Oxford University Press
DOI: 10.1093/synbio/ysab030

Enhanced resistance of Trichoderma harzianum LZDX-32-08 to hygromycin B induced by sea salt

Author(s): Xijia Yu, Huiling Wei, Xianrui Liu, Dong Liu, Aili Fan, Haijia Su
Published in: Biotechnology Letters, 43/1, 2021, Page(s) 213-222, ISSN 0141-5492
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10529-020-02994-y

Hyphenation of supercritical fluid chromatography with different detection methods for identification and quantification of liamocin biosurfactants

Author(s): Karen Scholz, Anna Lipphardt, Carina M. Wienken, Till Tiso, Heiko Hayen
Published in: Journal of Chromatography A, 1631, 2020, Page(s) 461584, ISSN 0021-9673
Publisher: Elsevier BV
DOI: 10.1016/j.chroma.2020.461584

Quantitative assessment of morphological traits of planktonic bacterial aggregates

Author(s): David R. Espeso, Esteban Martínez-García, Víctor de Lorenzo
Published in: Water Research, 188, 2021, Page(s) 116468, ISSN 0043-1354
Publisher: Elsevier BV
DOI: 10.1016/j.watres.2020.116468

Low CyaA expression and anti‐cooperative binding of cAMP to CRP frames the scope of the cognate regulon of Pseudomonas putida

Author(s): Alejandro Arce‐Rodríguez, Pablo I. Nikel, Belén Calles, Max Chavarría, Raúl Platero, Tino Krell, Victor Lorenzo
Published in: Environmental Microbiology, 23/3, 2021, Page(s) 1732-1749, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15422

Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling

Author(s): Nadia A. Samak, Yunpu Jia, Moustafa M. Sharshar, Tingzhen Mu, Maohua Yang, Sumit Peh, Jianmin Xing
Published in: Environment International, 145, 2020, Page(s) 106144, ISSN 0160-4120
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2020.106144

Rapid colonization and biodegradation of untreated commercial polyethylene wrap by a new strain of Bacillus velezensis C5

Author(s): Xianrui Liu, Yiming Zhang, Qiufeng Sun, Zihan Liu, Yilin Zhao, Aili Fan, Haijia Su
Published in: Journal of Environmental Management, 2021, ISSN 0301-4797
Publisher: Academic Press
DOI: 10.1016/j.jenvman.2021.113848

Genome analysis of the metabolically versatile Pseudomonas umsongensis GO16: the genetic basis for PET monomer upcycling into polyhydroxyalkanoates

Author(s): Tanja Narancic, Manuel Salvador, Graham M. Hughes, Niall Beagan, Umar Abdulmutalib, Shane T. Kenny, Huihai Wu, Marta Saccomanno, Jounghyun Um, Kevin E. O'Connor, José I. Jiménez
Published in: Microbial Biotechnology, 2021, ISSN 1751-7915
Publisher: WILEY
DOI: 10.1111/1751-7915.13712

Metabolic modelling approaches for describing and engineering microbial communities

Author(s): Beatriz García-Jiménez, Jesús Torres-Bacete, Juan Nogales
Published in: Computational and Structural Biotechnology Journal, 19, 2021, Page(s) 226-246, ISSN 2001-0370
Publisher: Elsevier B.V.
DOI: 10.1016/j.csbj.2020.12.003

A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria

Author(s): Gonzalo Durante-Rodríguez, Belén Calles, Víctor Lorenzo, Pablo Nikel
Published in: BIO-PROTOCOL, 10/14, 2020, ISSN 2331-8325
Publisher: Bio-101
DOI: 10.21769/bioprotoc.3688

The faulty SOS response of Pseudomonas putida KT2440 stems from an inefficient RecA‐LexA interplay

Author(s): Özlem Akkaya, Tomás Aparicio, Danilo Pérez‐Pantoja, Víctor Lorenzo
Published in: Environmental Microbiology, 23/3, 2021, Page(s) 1608-1619, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15384

Rational flux-tuning of Halomonas bluephagenesis for co-production of bioplastic PHB and ectoine

Author(s): Hong Ma, Yiqing Zhao, Wuzhe Huang, Lizhan Zhang, Fuqing Wu, Jianwen Ye, Guo-Qiang Chen
Published in: Nature Communications, 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-17223-3

Applications of phage-derived RNA-based technologies in synthetic biology

Author(s): Wenhui Zhang, Qiong Wu
Published in: Synthetic and Systems Biotechnology, 5/4, 2020, Page(s) 343-360, ISSN 2405-805X
Publisher: Science Direct
DOI: 10.1016/j.synbio.2020.09.003

Defined Microbial Mixed Culture for Utilization of Polyurethane Monomers

Author(s): Romualdus Nugraha Catur Utomo, Wing-Jin Li, Till Tiso, Christian Eberlein, Moritz Doeker, Hermann J. Heipieper, Andreas Jupke, Nick Wierckx, Lars M. Blank
Published in: ACS Sustainable Chemistry & Engineering, 8/47, 2020, Page(s) 17466-17474, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.0c06019

Naked Bacterium: Emerging Properties of a Surfome-Streamlined Pseudomonas putida Strain

Author(s): Esteban Martínez-García, Sofía Fraile, David Rodríguez Espeso, Davide Vecchietti, Giovanni Bertoni, Víctor de Lorenzo
Published in: ACS Synthetic Biology, 9/9, 2020, Page(s) 2477-2492, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.0c00272

The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future

Author(s): Till Tiso, Benedikt Winter, RenWei, Johann Hee, Jan de Witt, NickWierckx, Peter Quicker, Uwe T. Bornscheuer, André Bardow, Juan Nogales, Lars M.Blank
Published in: Metabolic Engineering, 2021, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.12.006

Environmental Performance of Pseudomonas putida with a Uracylated Genome

Author(s): Elena Algar, Yamal Al‐Ramahi, Víctor Lorenzo, Esteban Martínez‐García
Published in: ChemBioChem, 21/22, 2020, Page(s) 3255-3265, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000330

Quantum Mechanical Investigation of the Oxidative Cleavage of the C–C Backbone Bonds in Polyethylene Model Molecules

Author(s): Qixuan Jiang, Zhongyu Li, Ziheng Cui, Ren Wei, Kaili Nie, Haijun Xu, Luo Liu
Published in: Polymers, 13/16, 2021, Page(s) 2730, ISSN 2073-4360
Publisher: MDPI
DOI: 10.3390/polym13162730

Insight into formation and biological characteristics of Aspergillus tubingensis-based aerobic granular sludge (AT-AGS) in wastewater treatment

Author(s): Yingyun Chen, Jiye Ge, Shaojie Wang, Haijia Su
Published in: Science of The Total Environment, 739, 2020, Page(s) 140128, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2020.140128

Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies

Author(s): Sofía Fraile, María Briones, Mónica Revenga-Parra, Víctor de Lorenzo, Encarnación Lorenzo, Esteban Martínez-García
Published in: ACS Synthetic Biology, 10/8, 2021, Page(s) 2049-2059, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.1c00227

Defined Microbial Mixed Culture for Utilization of Polyurethane Monomers

Author(s): Romualdus Nugraha Catur Utomo, Wing-Jin Li, Till Tiso, Christian Eberlein, Moritz Doeker, Hermann J. Heipieper, Andreas Jupke, Nick Wierckx, Lars M. Blank
Published in: ACS Sustainable Chemistry & Engineering, 8/47, 2020, Page(s) 17466-17474, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.0c06019

Nano-immobilization of PETase enzyme for enhanced polyethylene terephthalate biodegradation

Author(s): Jia, Yunpu Samak, Nadia A. Hao, Xuemi Chen, Zheng Yang, Gama Zhao, Xuhao Mu, Tingzhen Yang, Maohua Xing, Jianmin
Published in: Biochemical Engineering Journal, 2021, ISSN 1369-703X
Publisher: Elsevier BV
DOI: 10.1016/j.bej.2021.108205

Contextual dependencies expand the re-usability of genetic inverters

Author(s): Huseyin Tas; Lewis Grozinger; Ruud Stoof; V de Lorenzo; Angel Goñi-Moreno
Published in: Nature Communications, 3, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1101/2020.07.15.204651

An automated DIY framework for experimental evolution of Pseudomonas putida

Author(s): David R. Espeso, Pavel Dvořák, Tomás Aparicio, Víctor Lorenzo
Published in: Microbial Biotechnology, 2020, Page(s) 1-7, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13678

Subcellular Architecture of the xyl Gene Expression Flow of the TOL Catabolic Plasmid of Pseudomonas putida mt-2

Author(s): Juhyun Kim, Angel Goñi-Moreno, Víctor de Lorenzo
Published in: mBio, 12/1, 2021, ISSN 2161-2129
Publisher: American Society for Microbiology
DOI: 10.1128/mbio.03685-20

When microbial biotechnology meets material engineering

Author(s): Ana M. Hernandez-Arriaga, Cristina Campano, Virginia Rivero-Buceta and M. Auxiliadora Prieto
Published in: Microbial Biotechnology, 2021, 2021, Page(s) 1-15, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13975

Mechanism-Based Design of Efficient PET Hydrolases

Author(s): Ren Wei, Gerlis von Haugwitz, Lara Pfaff, Jan Mican, Christoffel P. S. Badenhorst, Weidong Liu, Gert Weber, Harry P. Austin, David Bednar, Jiri Damborsky, Uwe T. Bornscheuer
Published in: ACS Catalysis, 2022, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c05856

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Author(s): Li, Wing Jin; Narancic, Tanja; Kenny, Shane T.; Niehoff, Paul-Joachim; O’Connor, Kevin; Blank, Lars M.; Wierckx, Nick
Published in: Frontiers in Microbiology, 19, 2020, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.18154/rwth-2020-05721

Automated design and implementation of a NOR gate in Pseudomonas putida

Author(s): Huseyin Tas, Lewis Grozinger, Angel Goñi-Moreno, Victor de Lorenzo
Published in: Synthetic Biology, Volume 6, Issue 1, 2021, ISSN 2397-7000
Publisher: Oxford University Press
DOI: 10.1093/synbio/ysab024

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Author(s): Wing-Jin Li, Tanja Narancic, Shane T. Kenny, Paul-Joachim Niehoff, Kevin O’Connor, Lars M. Blank, Nick Wierckx
Published in: Frontiers in Microbiology, 11, 2020, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2020.00382

Recent Advances in Bioplastics: Application and Biodegradation

Author(s): Tanja Narancic, Federico Cerrone, Niall Beagan, Kevin E. O’Connor
Published in: Polymers, 12/4, 2020, Page(s) 920, ISSN 2073-4360
Publisher: MDPI
DOI: 10.3390/polym12040920

Biodegradation of low-density polyethylene by Microbulbifer hydrolyticus IRE-31

Author(s): Zhongyu Li, Ren Wei, Meixi Gao, Yanru Ren, Bo Yu, Kaili Nie, Haijun Xu, Luo Liu
Published in: Journal of Environmental Management, 263, 2020, Page(s) 110402, ISSN 0301-4797
Publisher: Academic Press
DOI: 10.1016/j.jenvman.2020.110402

Thermophilic whole‐cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum

Author(s): Fei Yan, Ren Wei, Qiu Cui, Uwe T. Bornscheuer, Ya‐Jun Liu
Published in: Microbial Biotechnology, 2020, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd
DOI: 10.1111/1751-7915.13580

Anti-staphylococcal hydrogels based on bacterial cellulose and the antimicrobial biopolyester poly(3-hydroxy-acetylthioalkanoate-co-3-hydroxyalkanoate)

Author(s): Virginia Rivero-Buceta, María Rosa Aguilar, Ana María Hernández-Arriaga, Francisco G. Blanco, Antonia Rojas, Marta Tortajada, Rosa Ana Ramírez-Jiménez, Blanca Vázquez-Lasa, Auxiliadora Prieto
Published in: International Journal of Biological Macromolecules, 2020, ISSN 0141-8130
Publisher: Elsevier BV
DOI: 10.1016/j.ijbiomac.2020.07.289

A Combined Bio-Chemical Synthesis Route for 1-Octene Sheds Light on Rhamnolipid Structure

Author(s): Till Tiso, Daniel F. Sauer, Klaus Beckerle, Christian C. Blesken, Jun Okuda, Lars M. Blank
Published in: Catalysts, 10/8, 2020, Page(s) 874, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal10080874

Impact of the number of rhamnose moieties of rhamnolipids on the structure, lateral organization and morphology of model biomembranes

Author(s): Marius Herzog; Lei Li; Christian C. Blesken; Gina Welsing; Till Tiso; Lars M. Blank; Roland Winter
Published in: Soft Matter, 4, 2021, Page(s) 3191-3206, ISSN 1744-683X
Publisher: Royal Society of Chemistry
DOI: 10.18154/rwth-2021-04514

Biocatalysis in the Recycling Landscape for Synthetic Polymers and Plastics towards Circular Textiles

Author(s): Christina Jönsson, Ren Wei, Antonino Biundo, Johan Landberg, Lisa Schwarz Bour, Fabio Pezzotti, Andreea Toca, Les M. Jacques, Uwe T. Bornscheuer, Per‐Olof Syrén
Published in: ChemSusChem, 2021, ISSN 1864-5631
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.202002666

Biosensor and chemo-enzymatic one-pot cascade applications to detect and transform PET-derived terephthalic acid in living cells

Author(s): Thomas Bayer, Lara Pfaff, Yannick Branson, Aileen Becker, Shuke Wu, Uwe T.Bornscheuer, Ren Wei
Published in: iScience, 2022, ISSN 2589-0042
Publisher: Elsevier Inc.
DOI: 10.1016/j.isci.2022.104326

Recent advances in the improvement of bi-directional electron transfer between abiotic/biotic interfaces in electron-assisted biosynthesis system

Author(s): Bin Xu, Zhe Li, Yujia Jiang, Minjiao Chen, Boryann Chen, Fengxue Xin, Weiliang Dong, Min Jiang
Published in: Biotechnology Advances, 2021, Page(s) 107810, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/j.biotechadv.2021.107810

Engineering and evaluation of thermostable IsPETase variants for PET degradation

Author(s): Stefan Brott, Lara Pfaff, Josephine Schuricht, Jan-Niklas Schwarz, Dominique Böttcher, Christoffel P. S. Badenhorst, Ren Wei, Uwe T. Bornscheuer
Published in: Engineering in Life Sciences, 2021, ISSN 1618-2863
Publisher: John Wiley & Sons, Inc.
DOI: 10.1002/elsc.202100105

Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling

Author(s): Nadia A. Samak, Yunpu Jia, Moustafa M. Sharshar, Tingzhen Mu, Maohua Yang, Sumit Peh, Jianmin Xing
Published in: Environment International, 145, 2020, Page(s) 106144, ISSN 0160-4120
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envint.2020.106144

Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects

Author(s): Jiawei Liu, Jie He, Rui Xue, Bin Xu, Xiujuan Qian, Fengxue Xin, Lars M. Blank, Jie Zhou, Ren Wei, Weiliang Dong, Min Jiang
Published in: Biotechnology Advances, 48, 2021, Page(s) 107730, ISSN 0734-9750
Publisher: Elsevier BV
DOI: 10.1016/j.biotechadv.2021.107730

Engineering adipic acid metabolism in Pseudomonas putida

Author(s): Yannic S. Ackermann, Wing-Jin Li, Leonie Op de Hipt, Paul-Joachim Niehoff, William Casey, Tino Polen, Sebastian Köbbing, Hendrik Ballerstedt, Benedikt Wynands, Kevin O'Connor, Lars M. Blank, Nick Wierckx
Published in: Metabolic Engineering, 67, 2021, Page(s) 29-40, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.05.001

The Wsp intermembrane complex mediates metabolic control of the swim‐attach decision of Pseudomonas putida

Author(s): Ángeles Hueso‐Gil, Belén Calles, Víctor Lorenzo
Published in: Environmental Microbiology, 22/8, 2020, Page(s) 3535-3547, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15126

Recombinant protein production in the filamentous fungus Trichoderma

Author(s): Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su
Published in: Chinese Journal of Chemical Engineering, 2020, ISSN 1004-9541
Publisher: Chemical Industry Press
DOI: 10.1016/j.cjche.2020.11.006

Comparative Analysis of the Behaviour of Marine Litter in Thermochemical Waste Treatment Processes

Author(s): Johann Hee, Kai Schlögel, Simone Lechthaler, Jacqueline Plaster, Kristina Bitter, Lars Mathias Blank, Peter Quicker
Published in: Processes, 9/1, 2021, Page(s) 13, ISSN 2227-9717
Publisher: MDPI
DOI: 10.3390/pr9010013

An automated DIY framework for experimental evolution of Pseudomonas putida

Author(s): David R. Espeso, Pavel Dvořák, Tomás Aparicio, Víctor Lorenzo
Published in: Microbial Biotechnology, 2020, Page(s) 1-7, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology
DOI: 10.1111/1751-7915.13678

Granulation enhancement and microbial community shift of tylosin-tolerant aerobic granular sludge on the treatment of tylosin wastewater

Author(s): Wei Xiong, Shaojie Wang, Nan Zhou, Yingyun Chen, Haijia Su
Published in: Bioresource Technology, 318, 2020, Page(s) 124041, ISSN 0960-8524
Publisher: Elsevier BV
DOI: 10.1016/j.biortech.2020.124041

Picking the right metaphors for addressing microbial systems: economic theory helps understanding biological complexity

Author(s): Juhyun Kim, Rafael Silva-Rocha, Víctor de Lorenzo
Published in: International Microbiology, 2021, ISSN 1139-6709
Publisher: Sociedad Espanola de Microbiologia
DOI: 10.1007/s10123-021-00194-w

Grand Challenges for Industrializing Polyhydroxyalkanoates (PHAs)

Author(s): Dan Tan, Ying Wang, Yi Tong, Guo-Qiang Chen
Published in: Trends in Biotechnology, 39/9, 2021, Page(s) 953-963, ISSN 0167-7799
Publisher: Elsevier BV
DOI: 10.1016/j.tibtech.2020.11.010

Transcriptional control of 2,4‐dinitrotoluene degradation in Burkholderia sp . R34 bears a regulatory patch that eases pathway evolution

Author(s): Danilo Pérez‐Pantoja, Pablo I. Nikel, Max Chavarría, Víctor Lorenzo
Published in: Environmental Microbiology, 23/5, 2021, Page(s) 2522-2531, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.15472

Seawater activated TiO2 photocatalyst for degradation of organic compounds

Author(s): Max-Fabian Volhard, Jonas Johannes Christ, Lars Mathias Blank, Thomas Jüstel
Published in: Sustainable Chemistry and Pharmacy, 16, 2020, Page(s) 100251, ISSN 2352-5541
Publisher: Elsevier
DOI: 10.1016/j.scp.2020.100251

MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020

Author(s): Ballerstedt, Hendrik; Tiso, Till; Wierckx, Nick; Wei, Ren; Averous, Luc; Bornscheuer, Uwe; O’Connor, Kevin; Floehr, Tilman; Jupke, Andreas; Klankermayer, Jürgen; Liu, Luo; de Lorenzo, Victor; Narancic, Tanja; Nogales, Juan; Perrin, Rémi; Pollet, Eric; Prieto, Auxiliadora; Casey, William; Haarmann, Thomas; Sarbu, Alexandru; Schwaneberg, Ulrich; Xin, Fengxue; Dong, Weiliang; Xing, Jiamin; Chen,
Published in: Microbial Biotechnology, 1, 2021, Page(s) 1-15, ISSN 1751-7915
Publisher: John Wiley & Sons Ltd and Society for Applied Microbiology.
DOI: 10.1111/1751-7915.13975

Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties

Author(s): Ziyu Wang, Qin Qin, Yifei Zheng, Fajin Li, Yiqing Zhao, Guo-Qiang Chen
Published in: Metabolic Engineering, 67, 2021, Page(s) 53-66, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.05.010

Converting petrochemical plastic to biodegradable plastic

Author(s): Narancic T., Wierckx N., Liu S., O'Connor K.E.
Published in: The Handbook of Polyhydroxyalkanoates – Microbial biosynthesis and feedstocks, 2020, ISBN 9780429296611
Publisher: CRC press, Taylor & Francis Group

Fluorimetric high-throughput screening method for polyester hydrolase activity using polyethylene terephthalate nanoparticles

Author(s): Lara Pfaff, Daniel Breite, Christoffel P.S. Badenhorst, Uwe T. Bornscheuer, Ren Wei
Published in: Enzymatic Plastic Degradation, 648, 2021, Page(s) 253-270, ISSN 0076-6879
Publisher: Academic Press
DOI: 10.1016/bs.mie.2020.11.003

Anchor peptides promote degradation of mixed plastics for recycling

Author(s): Yu Ji, Yi Lu, Hendrik Puetz, Ulrich Schwaneberg
Published in: Enzymatic Plastic Degradation, 648, 2021, Page(s) 271-292, ISBN 9780128220122
Publisher: Elsevier
DOI: 10.1016/bs.mie.2020.12.027

Upcycling of hydrolyzed PET by microbial conversion to a fatty acid derivative

Author(s): Gina Welsing, Birger Wolter, Henric M.T. Hintzen, Till Tiso, Lars M. Blank
Published in: Enzymatic Plastic Degradation, 648, 2021, Page(s) 391-421, ISBN 9780128220122
Publisher: Elsevier
DOI: 10.1016/bs.mie.2020.12.025

Großartige Winzlinge - Mikroben sind die heimlichen Herrscher der Welt. Und wir können sie für vieles nutzen.

Author(s): Magdalena Hamm, Susan Schädlich, Anja Garms, Michael Stang
Published in: Das Magazin für neugierige Forscher, "#1, April 2020", 2020, Page(s) 2-5
Publisher: Bundesministerium für Bildung und Forschung (BMBF)

Waste to plastics by enzymes and bacteria - From plastic waste to PHA using enzymes and the bacterium P. putida

Author(s): MT
Published in: bioplastics MAGAZINE, 02/20 Vol. 15, 2020, Page(s) 22-24
Publisher: Polymedia Publisher GmbH