The project was divided into three scientific work packages.
WP1 – Vine adaptation to climate change: A comparison of over 200 Malbec clones with contrasting traits related to climate change adaptation enabled the evaluation of natural genetic variation. Proximal sensing technologies on an unmanned ground vehicle were used for plant scanning and phenotyping. Cell wall composition analysis protocols specific to grape samples were optimized to identify variants better adapted to climate change. The genetic analysis of Criolla Chica nº2, a somatic variant of ‘Listán Prieto’, and comparison with a similar 'Tempranillo' variant aimed to confirm or refute candidate loci through whole-genome sequencing. ABA application was effective for producing high-quality table grapes in Sicily, and gene expression of transcription factors, enzymes, and two aromatic pathway genes was studied. Climate change impacts on grape composition require better water resource management and understanding of heat waves, irrigation volumes during phenological phases, and their physiological and chemical effects. A protocol for extracting polyunsaturated fatty acids (PUFA), influenced by vine stress, was optimized and tested on Sauvignon blanc under two light stress conditions, showing metabolic differences.
WP2 – Grape and wine composition for quality and authenticity: A study on reducing alcohol content in Chardonnay via sequential fermentation or water addition (5%, 10%) was conducted. Key markers, including proteins and aromas, were analyzed, along with sensory and consumer tests. Correlation analysis is ongoing. Wine composition under various climates and ripening treatments was investigated. IR spectroscopy was evaluated against traditional methods for its principles, limitations, and potential applications. Tools for assessing wine authenticity were optimized. Wine production monitoring methods were improved using robust scientific methodologies. A method for PUFA profiling in grape berries under abiotic stress was validated, and extensions to carotenoids and volatile markers are ongoing to link them to grape quality. Studies focused on grape development under heat waves and on micro-winemaking emphasized grape composition.
WP3 – Winemaking innovation for sustainability and safety: Results included defining a microbiome sampling protocol in Argentine vineyards, analyzing metagenomic and metabolomic traits of local yeast strains (Saccharomyces cerevisiae and Torulaspora delbrueckii), and evaluating ethanol reduction techniques and their impact on malolactic fermentation. Pied-de-cuve fermentation was used to preserve native microbiota and enhance wine microbial and sensory profiles. Knowledge transfer between academia and industry aimed to improve the transferability of yeast strains. Studies also addressed nitrogen metabolism in yeast and their modification potential for producing oenologically valuable compounds.
The vWISE project produced a guidance manual to support winegrowers in adapting viticulture and winemaking to climate change, and generated a wine authenticity database. Scientific output included 13 international journal articles, 38 participations in scientific events, and 30 other external conferences and workshops.