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A Multiscale Analysis of Bacterial Predation

Project description

Studying predatory bacteria

The EU-funded JAWS project aims to study predatory bacteria, and particularly the molecular, cellular and ecological aspects of collective bacterial predatory behaviour. The project will focus on how Myxococcus xanthus invades prey colonies and directly feeds on the prey cells by contact-dependent killing. It will also investigate how the Myxococcus cells disseminate throughout the prey colony as concerted waves to prepare the next predatory cycle. The methodology of the project will be based on the development of a new imaging tool, Bacto-Hubble, which will capture the entire predatory cycle at single-cell resolution. Eventually, the project will produce a blueprint of the Myxococcus predatory cycle, which will shed light on other multicellular predatory processes.

Objective

Predation arguably impacts the evolution and shape of ecological systems. This process occurs at all scales from mammals to viruses. Predatory bacteria remain poorly studied despite their biological importance and often versatile experimental tractability. In this project, we propose to remedy this gap and study the molecular, cellular and ecological aspects of a collective bacterial predatory behavior. Specifically, capitalizing from previous molecular studies on motility, we will investigate how Myxococcus xanthus invades prey colonies and directly feeds on the prey cells by contact-dependent killing. We will then explore how the Myxococcus cells disseminate throughout the prey colony as concerted waves, thus preparing the next predatory cycle. While these experiments will be conducted in laboratory context, we will link molecular mechanisms of predation to ecological dynamics that actually occur in the wild directly by genomic analyses of environmental isolates and experimental evolution of predator-prey interactions. The feasibility of the project is guaranteed by the development of a new imaging tool (Bacto-Hubble) that captures the entire predatory cycle at single cell resolution. In addition, our laboratory recently identified for the first time the Myxococcus genes that promote contact-dependent killing, paving to molecular studies of prey killing and consumption. This project is uniquely multiscale and its realization requires true interdisciplinarity. For this, we will assemble a large collaborative network to connect approaches from the realms of genetics, structural biochemistry, theory and modeling and large-scale genomics. At the end of the project, we expect to obtain a first spatially resolved molecular blueprint of the Myxococcus predatory cycle, which will provide a framework to understand other multicellular predatory processes and well as their evolutionary trajectories.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-ADG - Advanced Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2019-ADG

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Host institution

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 370 352,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 370 352,00

Beneficiaries (1)

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