Descripción del proyecto
Regulación de la cromatina en la hematopoyesis
Los factores de cromatina determinan el destino celular y la decisión del desarrollo de linajes participando en complejos dinámicos de cromatina y regulando la expresión génica. Dado que los factores de cromatina están entre las proteínas mutadas con mayor frecuencia en diversas neoplasias hematológicas, como por ejemplo en la leucemia de linaje mixto, el proyecto financiado con fondos europeos ChRONAM-H tiene como objetivo determinar el papel de estas proteínas en el compromiso del linaje hematopoyético. Mediante tecnologías punteras de identificación de la cromatina, los científicos vincularán factores de cromatina con factores de transcripción tanto en hematopoyesis normal como maligna. Los resultados brindarán información fundamental sobre los mecanismos reguladores del genoma e identificarán dianas moleculares para combatir la leucemia.
Objetivo
Chromatin Factors (CFs) comprise a diverse family of proteins that elicit key regulatory epigenetic activities during the regulation of cellular fates. Although this heterogeneous group has been traditionally considered a static toolkit of epigenetic enzymes deployed to lineage-specific loci under the control of Transcription Factors (TFs), recent works have shown that CFs are crucial determinants of developmental lineage decisions that assemble into dynamic chromatin regulatory complexes with exquisite cell-type-specific composition. Hematopoiesis is a key developmental process largely dependent on Chromatin Factors. Importantly, CFs are the most recurrently mutated protein category across diverse blood malignancies. In fact, alterations in some of these proteins, like MLL fusions, have been proven to be central drivers of malignant transformation. However, in spite of this proven relevance, the contribution of most Chromatin Factors to blood lineage commitment remains largely unexplored; moreover, even for well-studied complexes such as SWI/SNF Polycomb or COMPASS, very little is known about the mechanisms that govern their locus-specific assembly and activity during cell fate specification.
My project Chromatin Regulation Of Normal And Malignant Haematopoiesis (ChRONAM-H) proposes a multidisciplinary framework to explore the functions of CFs during hematopoietic lineage commitment and malignant transformation. I will combine high-throughput functional measurements with state-of-the-art chromatin profiling technologies to unveil the lineage specifying roles of a large number of CFs and orthogonally link them within existing TF-centric networks in both normal and malignant hematopoiesis. This will shed light on the molecular behavior of chromatin regulatory complexes, providing new insights about the genome regulatory mechanisms that orchestrate hematopoiesis and how they might be therapeutically targeted to fight leukemia.
Ámbito científico
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Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
CB2 1TN Cambridge
Reino Unido