Descripción del proyecto
Una mejor comprensión de las propiedades biomoleculares de las bacterias de anammox
Para evitar la mortandad de peces, es necesario eliminar por completo el amoníaco y otros compuestos de nitrógeno de los desechos humanos en las depuradoras de aguas residuales y las bacterias de anammox pueden realizar esta transformación. Sin embargo, debido a la ausencia de estudios moleculares para entender a estas bacterias y su comportamiento, aún hay importantes aplicaciones tecnológicas en investigación. Comprender cómo logran encontrar las diferentes especies de anammox su espacio ecológico particular no es tarea sencilla. El proyecto financiado con fondos europeos RESPIMMOX tiene el objetivo de mejorar los conocimientos sobre las propiedades biomoleculares de «Candidatus brocadia», el principal género con capacidad anammox en las depuradoras de aguas residuales empleado para mejorar el rendimiento de los biorreactores. El proyecto desarrollará un cultivo enriquecido con «Candidatus brocadia anammoxidans» mediante grandes concentraciones de células. También identificará y caracterizará los complejos proteicos que participan en el metabolismo energético de la «Candidatus brocadia anammoxidans».
Objetivo
The finding of anammox bacteria brought up the possibility of achieving energy-self-sufficient wastewater treatment plants for nitrogen removal, but their widespread technological application is still under research. The lack of pure cultures, standard cultivation methods and comprehensive genetic data make the molecular research on anammox bacteria challenging, and thus, there is a critical absence of molecular studies to understand how these bacteria make a living. One of the current challenges of anammox bacteria research is to understand niche differentiation, meaning how and why different anammox species find their unique ecological space. RESPIMMOX project aims to understand the biomolecular characteristics of anammox Ca. Brocadia that trigger their niche differentiation as the main anammox genus present in wastewater treatment plants, to improve the efficiency and stability of bioreactors. We will obtain biochemical evidence of the membrane-bound protein complexes involved in the electron transport chain of Brocadia-species, and investigate the soluble yet-unidentified enzymes responsible of the nitrite reduction. We will follow a complexome profiling approach combined with enzymatic activity assays, as commonly performed with Dehalococcoides mccartyi in the host laboratory. RESPIMMOX project will be accomplished through three specific objectives: i) achievement of an anammox Brocadia-enriched culture with high cell concentrations; ii) training of the researcher in biochemical methods to characterize respiratory complexes using the respiration with halogenated aromatic compounds as an example; and iii) identification and characterization of the protein complexes involved in the anammox Ca. Brocadia energy metabolism. The results from this proposal will entail a step forward for the understanding of anammox niche differentiation, specifically of the dominance of Ca. Brocadia in bioreactors, and will contribute to unravel the puzzle that anammox bacteria pose.
Ámbito científico
- engineering and technologyenvironmental engineeringwater treatment processeswastewater treatment processes
- engineering and technologyenvironmental biotechnologybioremediationbioreactors
- natural sciencesbiological sciencesmicrobiologybacteriology
- natural scienceschemical sciencesorganic chemistryaromatic compounds
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Palabras clave
Programa(s)
Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
04318 Leipzig
Alemania