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Glycans coated nanoparticles as a biocompatible shield to increase nanoparticles colloidal stability and modulate their immunological response.

Project description

Glycans to shield nanoparticles from the immune system

Nanoparticles have the potential to address current challenges in the diagnosis and treatment of several diseases as well as the vaccines against them. However, they exhibit limited stability and are recognised by the immune system, which leads to reduced targeting efficacy. Upon contact with biological fluids, nanoparticles acquire proteins on their surface, known as the protein corona. To overcome this problem, the EU-funded Glyco-NPs project proposes to synthesise a range of nanoparticles decorated with synthetic and naturally occurring glycans on their surface. This will not only prevent unspecific protein interaction but also deter immune responses and improve the bloodstream circulation half-life of nanoparticles.

Objective

Nanomedicine is currently the most studied and promising topics in the field of biomedical technology, which is focused on developing nanoparticles. The reason for this is that these materials present unique properties such as size, high ratio area/ volume, chemical reactivity, that offer potential solutions for many of the current challenges in diagnosis, therapy and vaccines on several diseases. However, the safe use of NPs in clinical applications is not established yet. At present, the NPs have limited stability in complex cell media, enhanced recognition by the immunological system and reduced targeting efficacy as a result of the protein corona formation. Studies in Dr. Monopoli´s laboratory at RSCI have demonstrated that glycosylation of the protein corona plays an important role in maintaining the colloidal stability of nanoparticles and influences nanoparticle-cell interactions. The proposed research programme will utilize the combination of interdisciplinary and intersectoral approaches (synthetic chemistry, physical chemistry, glycoprofiling and proteomics, an inter-sectoral secondment (CIC biomaGUNE, Prof Sergio Moya to learn fluorescence correlation spectroscopy and test the NP targeting efficacy) to synthetise a range of nanoparticles (NPs) decorated with synthetic and naturally occurring glycans (hence, Glyco-NPs) in order to: 1), increase the NPs' colloidal stability, 2) attenuate the unspecific protein interaction and 3) increase the NPs circulation half-life into the bloodstream. Together with my supervisor, we have designed a training plan that includes new research (glycoprofiling and bio-nanointeractions), and transferable (project management, leadership, innovation) skills.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2019

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Coordinator

ROYAL COLLEGE OF SURGEONS IN IRELAND
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 196 590,72
Address
ST STEPHEN'S GREEN 123
2 DUBLIN
Ireland

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Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 196 590,72
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