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Investigating the transcriptional regulation of auxin biosynthesis in Arabidopsis embryo

Project description

Unlocking the mechanisms that keep plant embryos healthy and growing despite the heat

Auxin is a plant growth hormone with several roles in plant growth and development including embryo axis formation, vascularisation and cell elongation. Embryonic auxin biosynthesis appears to be controlled on both spatial and temporal levels, suggesting the presence of regulatory networks. Since auxin acts as an integrator of environmental cues, this process also seems to be affected by elevated temperatures, resulting in impaired seed production. Given the trend of global warming and the increased difficulties of the agricultural sector in keeping up with growing food demand, the EU-funded ITRABAE project is studying the mechanisms of the spatiotemporal regulation of auxin genes during embryo development and the effect of heat stress. Better understanding could help flowering plants produce healthy seeds in the face of rising global temperatures.

Objective

Survival of flowering plants is determined by the proper production of seed, whose shape and size are defined by the development of an embryo. Such mature embryo mimics the body pattern of the new plant that will develop after germination. Auxin is a key regulator of various aspects of plant development, including embryo morphogenesis. Cellular auxin gradients, maintained by auxin production along with signalling and transport, are essential for proper embryo development. The embryonic local auxin biosynthesis appears spatiotemporally controlled, necessitating the presence of regulatory networks. Also, it became evident that auxin plays a critical role as an integrator of environmental cues, notably high temperatures, known to impair seed production. Thus, I aim at deciphering the molecular and genetic mechanisms behind the spatiotemporal regulation of auxin biosynthetic genes during embryo development, and how this regulatory network is impacted by heat stress. In the proposed work, I will functionally dissect the contribution of AGL transcription factors, identified in a yeast one-hybrid screen as binding to auxin biosynthetic promoters, to the direct regulation of auxin biosynthetic pathways using multidisciplinary approaches such as genetics, metabolomics, and molecular biology. Further, I will investigate whether high temperatures impact this regulation, notably at epigenetic levels. Overall, the proposed work would serve as an excellent foundation to dissect the mechanisms responsible for local auxin biosynthesis during embryo development, which would pave the way to devise strategies for mitigating the effects of climate warming on seed and plant morphogenesis.

Coordinator

Masarykova univerzita
Net EU contribution
€ 144 980,64
Address
Zerotinovo namesti 9
601 77 Brno
Czechia

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Region
Česko Jihovýchod Jihomoravský kraj
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 144 980,64