Description du projet
Une nouvelle sonde portable de spectromètre de masse pour le diagnostic in vivo du cancer de la peau
La spectrométrie de masse (SM) ambiante a récemment suscité de l’intérêt dans de nombreux domaines de recherche, notamment dans le diagnostic clinique, comme approche simple, rapide et peu invasive. Le projet SKIN-CANCER-PROBE, financé par l’UE, propose de développer une nouvelle sonde portable dotée d’un mécanisme de microextraction pour de petites molécules et pouvant être combinée à la SM pour effectuer des analyses en temps réel. Cette sonde sera utilisée comme outil diagnostique pour la détection rapide et peu invasive du cancer de la peau. Elle sera conçue comme un dispositif d’échantillonnage de surface, adapté à l’analyse ponctuelle et à l’imagerie chimique de la surface en mode continu. Ce dispositif sera en mesure d’effectuer une désorption à l’aide de l’union des solvants, et les métabolites désorbés seront dirigés vers la SM. Les biomarqueurs du cancer de la peau seront ciblés pendant le processus de développement de la sonde comme validation du concept.
Objectif
Ambient mass spectrometry (AMS) has gained substantial attention in numerous research fields including clinical diagnosis as being a
nondestructive, fast and simple approach. Despite of the advances in mass spectrometry (MS) one of its main disadvantages is the simultaneous introduction of the matrix and analyte to MS that results in low sensitivity associated with a high background and ion suppression, and frequent instrumental maintenance. Therefore, there is a need for a ground-breaking sampling tool that is combinable with MS and that does not compromise the above mentioned merits.
Here we propose (i) to develop a novel handheld probe, which will have micro extraction mechanism for small molecules/biomarkers from the surface and can be combined with MS for real time targeted and untargeted analyses; (ii) to utilize the probe as a clinical diagnostic tool for noninvasive fast determination of skin cancer. The probe will be designed as a surface touch sampling device suitable for spot analysis and for continuous mode chemical imaging of surface. The device will be integrated with online desorption using a solvent junction and desorbed metabolites will be directed to MS. As proof-of-concept, skin cancer biomarkers and some other typical skin metabolites will be targeted during the probe development. Preliminary analyses with the probe will be conducted on the surface of agarose gel spiked with the selected metabolites mimicking artificial skin surface using MS in targeted mode. Upon development of the probe and prove of the detection of the biomarkers from the synthetic skin media the study will be extended to in vivo mice model with tumor. This novel probe will make a significant impact as rapid and reliable diagnostic tool. This project will open an opportunity for the researcher to develop her future career by implementing the novel probe in emerging research fields (e.g. clinical, forensic, etc.), where fast and reliable analytical tools are in high demand.
Champ scientifique
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Régime de financement
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinateur
06800 Ankara
Turquie