Project description
Argonaute proteins and sRNA pathways
Small RNAs (sRNAs) serve as major players in a multitude of pathways, controlling development and preserving genome integrity in plants and animals. To perform their biological function, sRNAs are loaded to Argonaute (AGO) proteins, and the AGO/sRNA complexes recognise and regulate the target genes. The EU-funded AGOras project proposes a series of experimental approaches to study the transport routes and subcellular localisation and roles of AGO–sRNA complexes. Researchers plan to combine cell biology, RNA biochemistry, protein modelling, bioinformatics, deep-sequencing analyses, nuclear fractionation methods and single-molecule studies to identify key subcellular localisation signals of AGO proteins, elucidate AGO shuttling mechanisms and define the cytoplasmic and nuclear roles of AGO proteins in RNA pathways.
Objective
Small RNAs (sRNA) have emerged as major players in a multitude of pathways, controlling development and preserving genome integrity in plant and animals. To fulfill their biological function, sRNA are loaded into ARGONAUTE (AGO) proteins, together this AGO/sRNA complex recognizes and regulates the target genes. Depending on the organism and the sRNA pathway involved, AGOs have nuclear and/or the cytoplasmic localization and functions. Although globally AGO mediated processes are reasonably understood, the transport routes involved, their shuttling regulation and transient subcellular roles remain completely unknown. This proposal outlines a series of mutually reinforcing experiments to mechanistically study the transport routes and subcellular roles of AGO-sRNA complexes. In details, combining cell biology, RNA biochemistry, protein modeling, bioinformatics, deep-sequencing analyses, nuclear fractionation methods and single-molecule studies the proposed project aims to: 1) Identify key subcellular localization signals of AGO proteins; 2) Decipher AGO shuttling mechanism and their regulation; and 3) Dissect cytoplasmic and nuclear roles of AGO proteins on RNA pathways. The anticipated results will provide a crucial breakthrough in the eukaryotic RNA pathways and gene regulation fields. The interest in non-coding RNA action goes beyond mere mechanistic knowledge. Understanding non-coding RNA action is of paramount importance given the extraordinary potential held by the therapeutic, veterinary and agronomic exploitation of these molecules. As a result, the knowledge on the transport routes and subcellular roles of AGO-sRNA complexes obtained during the proposed project will provide an important contribution to European excellence and competitiveness in the field.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
08193 CERDANYOLA DEL VALLES
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.