Project description
Insight into cilia assembly and function
Cilia are conserved cell surface organelles composed of microtubules. They have diverse motility and sensory functions, and defects in their assembly lead to conditions known as ciliopathies. The EU-funded ResolvCiliaTip project will focus on the CCDC66 protein, a recently identified ciliary tip component implicated in progressive retinal degeneration, to provide insight into ciliary microtubule dynamics. Scientists aim to unveil the localisation and function of CCDC66, contributing to the improved characterisation of the causative genes of ciliopathies. Apart from providing fundamental knowledge, the project's results will lay the foundation for enhanced diagnostic approaches in future.
Objective
Cilia are conserved microtubule-based organelles, which extend from the cell surface and have diverse motility and sensory functions, integrating developmentally important signalling pathways. As such, defects in ciliary assembly and/or function can lead to developmental disorders and multi-systemic genetic diseases, termed ciliopathies. The ciliary tip, a region between microtubule ends and the ciliary membrane, is implicated in cilium assembly, disassembly, signalling, and motility. Few ciliary tip proteins identified so far are mutated in ciliopathies. Yet the complete identity of microtubule associated proteins at the ciliary tip of both primary and motile cilia, and the mechanisms underlying their regulation of ciliary microtubule dynamics and geometry, remain unknown. The proposed research will resolve the link between the ciliary tip geometry and function both in primary and motile cilia, as well as molecular mechanisms controlling the microtubule end dynamics at the ciliary tip and identify the full repertoire of ciliary tip components. The research will focus on functional characterization and interactions of the CCDC66 protein, which mutations cause progressive retinal degeneration, the most frequently observed phenotype across different ciliopathies. The CCDC66 is a recently identified tip component of the primary cilia and preliminary experiments suggest the axonemal and ciliary tip localization of CCDC66 in motile cilia. Therefore, we will investigate both the localization and function of CCDC66 at the primary and motile cilia tip with the nanometre-scale precision. We will elucidate the effect of CCDC66 on microtubule end dynamics using the biochemical assays and resolve the molecular proximity map of the CCDC66 using BioID method and proteomics. The results of this project, predicted to better characterize the causative genes of ciliopathies, could make positive impact on clinical studies and enhance diagnostic approaches in future.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences genetics mutation
- natural sciences mathematics pure mathematics geometry
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
34450 Istanbul
Türkiye
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.