TransEpiSeed: The role of mRNA methylation in control of seed germination

Información del proyecto

TransEpiSeed

Identificador del acuerdo de subvención: 897843

DOI

10.3030/897843

Proyecto cerrado

Fecha de la firma de la CE 24 Marzo 2020

Fecha de inicio 1 Enero 2021

Fecha de finalización 31 Diciembre 2022

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 207 312,00

Aportación de la UE

€ 207 312,00

Coordinado por

KOBENHAVNS UNIVERSITET
Denmark

Periodic Reporting for period 1 - TransEpiSeed (TransEpiSeed: The role of mRNA methylation in control of seed germination)

Período documentado: 2021-01-01 hasta 2022-12-31

Resumen del contexto y de los objetivos generales del proyecto

The project is aiming at revealing the molecular mechanism behind RNA methylation in the control of seed germination. Seed germination is essential for modern agriculture represented by mechanic sowing, which has high demands on homogenous germination with high seed vigor. The results from the current project will provide fundamental support in agricultural practice and plant breeding. The overall objectives are 1) Investigate methylation patterns for the ribosome-associated mRNA in seeds. 2) Conduct genome-wide profiling of m6A in mRNA at single nucleic acid resolution during seed germination.3) Examine the physiological role of carefully selected m6A “reader” protein in seeds. 4) Characterization of the molecular function of the m6A “reader” proteins.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

Work performed from project includes but not limited to plant molecular biology, plant genetics, plant genomics, bioinformitics etc. The main results include 1) Phenotypic and genomic screening the funtion of m6A reader proteins for their role in seed germination. 2) Identify the key m6A reader ECT2 and its mRNA targets during seed germination. 3) Expression analysis of ECT234 downstream genes. The project has been presented in several meeting and conference. The data generated from current result will be published in peer-reviewed journal and could be further explored for understanding the regulation of RNA methylation in seeds.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The results from the current project provide an overview of the role of m6A reader protein family played in seeds. The large impact of the TE234 axis on seed quality such as seed dormancy, longevity and stress tolerance. Due to the conservation of ECT family proteins, the result can be further explored in crops for breading better quality seeds under the background of global warming.

ECT2 reader protein expression in germinated seed as indicated by red fluorescence