TransEpiSeed: The role of mRNA methylation in control of seed germination

Informations projet

TransEpiSeed

N° de convention de subvention: 897843

DOI

10.3030/897843

Projet clôturé

Date de signature de la CE 24 Mars 2020

Date de début 1 Janvier 2021

Date de fin 31 Decembre 2022

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total

€ 207 312,00

Contribution de l’UE

€ 207 312,00

Coordonné par

KOBENHAVNS UNIVERSITET
Denmark

Periodic Reporting for period 1 - TransEpiSeed (TransEpiSeed: The role of mRNA methylation in control of seed germination)

Période du rapport: 2021-01-01 au 2022-12-31

Résumé du contexte et des objectifs généraux du projet

The project is aiming at revealing the molecular mechanism behind RNA methylation in the control of seed germination. Seed germination is essential for modern agriculture represented by mechanic sowing, which has high demands on homogenous germination with high seed vigor. The results from the current project will provide fundamental support in agricultural practice and plant breeding. The overall objectives are 1) Investigate methylation patterns for the ribosome-associated mRNA in seeds. 2) Conduct genome-wide profiling of m6A in mRNA at single nucleic acid resolution during seed germination.3) Examine the physiological role of carefully selected m6A “reader” protein in seeds. 4) Characterization of the molecular function of the m6A “reader” proteins.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

Work performed from project includes but not limited to plant molecular biology, plant genetics, plant genomics, bioinformitics etc. The main results include 1) Phenotypic and genomic screening the funtion of m6A reader proteins for their role in seed germination. 2) Identify the key m6A reader ECT2 and its mRNA targets during seed germination. 3) Expression analysis of ECT234 downstream genes. The project has been presented in several meeting and conference. The data generated from current result will be published in peer-reviewed journal and could be further explored for understanding the regulation of RNA methylation in seeds.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

The results from the current project provide an overview of the role of m6A reader protein family played in seeds. The large impact of the TE234 axis on seed quality such as seed dormancy, longevity and stress tolerance. Due to the conservation of ECT family proteins, the result can be further explored in crops for breading better quality seeds under the background of global warming.

ECT2 reader protein expression in germinated seed as indicated by red fluorescence