Periodic Reporting for period 2 - T-MEMORE (Thrombotic MEMOry-Linking a break in tolerance to platelets to REthrombosis)
Período documentado: 2022-06-01 hasta 2023-11-30
For WP2 I investigated the role of the spleen in coordinating the removal of activated platelets. Here, I show that splenectomy had no major impact on thrombus formation itself, but it was involved in the removal of activated platelets. In the liver only very few activated platelets are found after induction of venous thrombosis excluding a major role of this organ in platelet removal. However, in the context of vaccine associated thrombocytopenia I found that a vaccine vector binds to platelets in the setting of intravenous injection resulting in platelet activation and their removal by spleen macrophages (Nicolai et al., Blood 2022). Moreover, I have established an intravital microscopy setup as well as bioinformatic analysis approach to dissect platelet-leukocyte interactions by calcium imaging (Mehari et al, Science signaling, accepted)
The current understanding of the factors leading to thrombosis and its relapse are summarized in my recent review article in Nature Reviews Cardiology (Stark et al., Nat Rev Cardiol. 2021)