Switchable rhodOpsins in Life Sciences

Project Information

SOL

Grant agreement ID: 951644

DOI

10.3030/951644

EC signature date 7 February 2021

Start date 1 September 2022

End date 31 August 2028

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 7 971 388,75

EU contribution

€ 7 971 388,75

Coordinated by

PAUL SCHERRER INSTITUT
Switzerland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Open Research Data Pilot (1)

Data Management Plan (DMP)

Creation and curation of a DMP as prerequisite for taking part in the ORD pilot.

Publications

Peer reviewed articles (18)

Active state structures of a bistable visual opsin bound to G proteins

Author(s): Oliver Tejero, Filip Pamula, Mitsumasa Koyanagi, Takashi Nagata, Pavel Afanasyev, Ishita Das, Xavier Deupi, Mordechai Sheves, Akihisa Terakita, Gebhard F. X. Schertler, Matthew J. Rodrigues, Ching-Ju Tsai
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-53208-2

Retinal to Retinal Energy Transfer in a Bistable Microbial Rhodopsin Dimer

Author(s): Ivo H. M. van Stokkum, Jakub Dostal, Thanh Nhut Do, Lifei Fu, Gregor Madej, Christine Ziegler, Peter Hegemann, Miroslav Kloz, Matthias Broser, John T. M. Kennis
Published in: Journal of the American Chemical Society, Issue 147, 2025, Page(s) 14468-14480, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.5c01276

Beyond Lux: methods for species and photoreceptor-specific quantification of ambient light for mammals

Author(s): Richard J. McDowell, Altug Didikoglu, Tom Woelders, Mazie J. Gatt, Finn Moffatt, Saba Notash, Roelof A. Hut, Timothy M. Brown, Robert J. Lucas
Published in: BMC Biology, Issue 22, 2024, ISSN 1741-7007
Publisher: BioMed Central
DOI: 10.1186/s12915-024-02038-1

Spectral Tuning in Mammalian Melanopsins

Author(s): Richard J McDowell, Mazie J Gatt, Saba Notash, Robert J Lucas
Published in: Molecular Biology and Evolution, Issue 42, 2025, ISSN 0737-4038
Publisher: Oxford University Press
DOI: 10.1093/molbev/msaf158

Light-induced FTIR Spectroscopy of Visual Rhodopsin Microcrystals Grown in Lipidic Cubic Phase

Author(s): Yosuke Mizuno, Valérie Panneels, Yuji Furutani, Gebhard F.X. Schertler, Hideki Kandori, Kota Katayama
Published in: Journal of Molecular Biology, Issue 437, 2025, Page(s) 169487, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2025.169487

Activating an invertebrate bistable opsin with the all-trans 6.11 retinal analog

Author(s): Matthew J. Rodrigues, Oliver Tejero, Jonas Mühle, Filip Pamula, Ishita Das, Ching-Ju Tsai, Akihisa Terakita, Mordechai Sheves, Gebhard F. X. Schertler
Published in: Proceedings of the National Academy of Sciences, Issue 121, 2024, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2406814121

Study of Photoselectivity in Linear Conjugated Chromophores Using the XMS-CASPT2 Method

Author(s): Saumik Sen, Xavier Deupi
Published in: ACS Physical Chemistry Au, 2024, ISSN 2694-2445
Publisher: American Chemical Society
DOI: 10.1021/acsphyschemau.4c00065

Development of visible light‐sensitive human neuropsin (<scp>OPN5</scp>) via single amino acid substitution

Author(s): Yusuke Sakai, Richard J. McDowell, Robert J. Lucas
Published in: FEBS Letters, Issue 599, 2025, Page(s) 2612-2619, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.70130

A multiscale screening platform for rapid GPCR variant profiling reveals color-tuned JSR1 mutants

Author(s): Jonas Mühle, Deborah Walter, Marielouise Griebl, Gebhard F.X. Schertler
Published in: Biophysical Journal, 2025, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2025.10.034

Optogenetic interrogation of cell signalling: human neuropsin (hOPN5) represents a potent tool for controlling the Gq pathway with light

Author(s): Siri, Leemann; Sonja, Kleinlogel; Franziska, Schneider-Warme
Published in: Pflügers Archiv - European Journal of Physiology, Issue Volume 474, 2022, Page(s) 1217–1219, ISSN 0031-6768
Publisher: Springer Verlag
DOI: 10.1007/s00424-022-02765-w

Tool antibody fragments reveal multiple conformations of the rhodopsin-Gi signaling complex

Author(s): Filip Pamula, Oliver Tejero, Jonas Mühle, Ralf Thoma, Gebhard F.X. Schertler, Jacopo Marino, Ching-Ju Tsai
Published in: Biophysical Journal, 2025, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2025.09.044

Structural Insights Into the Opening Mechanism of C1C2 Channelrhodopsin

Author(s): Matthias Mulder, Songhwan Hwang, Matthias Broser, Steffen Brünle, Petr Skopintsev, Caspar Schattenberg, Christina Schnick, Sina Hartmann, Jonathan Church, Igor Schapiro, Florian Dworkowski, Tobias Weinert, Peter Hegemann, Han Sun, Jörg Standfuss
Published in: Journal of the American Chemical Society, Issue 147, 2025, Page(s) 1282-1290, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.4c15402

Ultrafast terahertz Stark spectroscopy reveals the excited-state dipole moments of retinal in bacteriorhodopsin

Author(s): Jia Zhang, Poonam Singh, Dieter Engel, Benjamin P. Fingerhut, Matthias Broser, Peter Hegemann, Thomas Elsaesser
Published in: Proceedings of the National Academy of Sciences, Issue 121, 2024, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2319676121

Multiple retinal isomerizations during the early phase of the bestrhodopsin photoreaction

Author(s): Spyridon Kaziannis, Matthias Broser, Ivo H. M. van Stokkum, Jakub Dostal, Wayne Busse, Arno Munhoven, Cesar Bernardo, Miroslav Kloz, Peter Hegemann, John T. M. Kennis
Published in: Proceedings of the National Academy of Sciences, Issue 121, 2024, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2318996121

Experimental Assessment of the Electronic and Geometrical Structure of a Near-Infrared Absorbing and Highly Fluorescent Microbial Rhodopsin

Author(s): Matthias Broser, Tadeusz Andruniów, Anastasia Kraskov, Riccardo Palombo, Sagie Katz, Miroslav Kloz, Jakub Dostál, César Bernardo, John T. M. Kennis, Peter Hegemann, Massimo Olivucci, Peter Hildebrandt
Published in: The Journal of Physical Chemistry Letters, Issue 14, 2023, Page(s) 9291-9295, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.3c02167

Diversity of rhodopsin cyclases in zoospore-forming fungi

Author(s): Matthias Broser; Wayne Busse; Anika Spreen; Maila Reh; Yinth Andrea Bernal Sierra; Songhwan Hwang; Tillmann Utesch; Han Sun; Peter Hegemann
Published in: Proceedings of the National Academy of Sciences, Issue Vol. 120 | No. 44, 2023, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2310600120

Graph-based algorithms to dissect long-distancewater-mediated H-bond networks for conformationalcouplings in GPCRs

Author(s): Eva Bertalan, Matthew Joseph Rodrigues, Gebhard F. X. Schertler, Ana-Nicoleta Bondar
Published in: British Journal of Pharmacology, 2024, ISSN 0007-1188
Publisher: Wiley-Blackwell
DOI: 10.1111/bph.16387

Multistep 11-<i>cis</i> to All-<i>trans</i> Retinal Photoisomerization in Bestrhodopsin, an Unusual Microbial Rhodopsin

Author(s): Matthias Broser, Spyridon Kaziannis, Ivo H.M. van Stokkum, Atripan Mukherjee, Jakub Dostal, Wayne Busse, Arno Munhoven, Cesar Bernardo, Peter Hegemann, Miroslav Kloz, John T.M. Kennis
Published in: Journal of the American Chemical Society, Issue 147, 2025, Page(s) 25571-25583, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.5c06216

Other (3)

Development of visible light-sensitive human OPN5 via single amino acid substitution

Author(s): Yusuke Sakai, Richard J. McDowell, Robert J. Lucas
Published in: bioRxiv, 2025
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.05.19.654910

Active state structures of a bistable visual opsin bound to G1proteins

Author(s): Oliver Tejero, Filip Pamula, Mitsumasa Koyanagi, Takashi Nagata, Pavel Afanasyev, Ishita Das, Xavier Deupi, Mordechai Sheves, Akihisa Terakita, Gebhard F.X. Schertler, Matthew J. Rodrigues, Ching-Ju Tsai
Published in: bioRxiv, 2024
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.04.09.588704

Beyond Lux: Methods for Species and Photoreceptor-Specific Quantification of Ambient Light for Mammals

Author(s): Richard J McDowell, Altug Didikoglu, Tom Woelders, Mazie J Gatt, Roelof A Hut, Timothy M Brown, Robert J Lucas
Published in: bioRxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.08.25.554794