Laser-induced hierarchical micro-/nano-structures for controlled cell adhesion at implants

Información del proyecto

LaserImplant

Identificador del acuerdo de subvención: 951730

DOI

10.3030/951730

Proyecto cerrado

Fecha de la firma de la CE 17 Junio 2020

Fecha de inicio 1 Enero 2021

Fecha de finalización 31 Marzo 2023

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 1 945 797,50

Aportación de la UE

€ 1 945 797,50

Coordinado por

UNIVERSITAT LINZ
Austria

Periodic Reporting for period 2 - LaserImplant (Laser-induced hierarchical micro-/nano-structures for controlled cell adhesion at implants)

Período documentado: 2022-01-01 hasta 2023-03-31

Resumen del contexto y de los objetivos generales del proyecto

A large application area of medical implants are currently dental prostheses – a rapidly growing market in ageing societies. These implants consisting of Ti or a Ti-alloy should provide good and fast osseointegration into the jaw bone. Opposed to that demand, in other applications (for instance for bone screws and plates), the implants may have to be removed after some months or several years and shall, therefore, not be completely overgrown by the bodies’ cells. Hence, a one-step laser-based surface functionalization of implant materials for controlling the cell growth is strongly desired.

The goal of the EIC Pathfinder pilot (FET-Proactive) project LaserImplant is the cooperation between academia, research centers, laser-processing device developers and implant producers for future development of smart medical implants addressing wide-spread patient’s needs in the fields of dental prostheses and screws and plates for bone regeneration. The project activities include to pave the pathway for commercialization of laser-functionalized implants, the exploration of industrial up-scaling strategies (including a demonstrator of implant functionalization machines to be conceived and built at the end of the project), and the dissemination and exploitation of the results.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

The adhesion of bone-forming osteoblast cells on Ti-based substrates can be controlled by a combination of laser-based surface functionalization and electrochemical anodization: either the cells can be activated or cell adhesion can be hindered.

In our publication (M. Muck et al., Nanomaterials 2021, 11, 1342), we were able to prove that microstructures and nanostructures can be used to reduce the adhesion of these cells on medical implants. While experiments with osteoblasts had shown that femtosecond laser-processing followed by electrochemical anodization resulted in an activation of the cells with increased production of extracellular matrix material, significant reduction of cell adhesion for osteoblasts was only obtained at pre-anodized surfaces. It could be demonstrated that this functionalization by means of femtosecond laser-processing can result in bone screws that hinder the adhesion of osteoblasts.

Laser-induced periodic surface structures (LIPSS) can influence the differentiation of human mesenchymal stem cells (hMSC) into the osteogenic direction. As is demonstrated in our recent publication (M. Maalouf at al., Nanomaterials 2022, 12, 1619), the best results are obtained by Radial LIPSS generated on Ti-based surfaces by azimuthal polarized femtosecond laser light. This fine tuning of ultrashort pulse lasers may be a promising new route for the functionalization of medical implants.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

Our vision is to develop smart medical implants, to pave the pathway for commercialization of laser-functionalized implants, and to implement a demonstrator of implant functionalization machines.

Cell-repellent Ti-based bone screw with blue pre-anodization; laser-processed rings shown in black.

Fluorescence staining; hMSC; Ti surfaces; LIPSS; adapted from M. Maalouf at al., Nanomaterials 2022