GUT VIrus BRain Axis Technology In OrgaNoid Science

Informations projet

GUTVIBRATIONS

N° de convention de subvention: 953201

DOI

10.3030/953201

Projet clôturé

Date de signature de la CE 18 Février 2021

Date de début 1 Avril 2021

Date de fin 31 Mars 2025

Financé au titre de

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies – Nanotechnologies

Coût total

Aucune donnée

Contribution de l’UE

€ 6 061 375,00

Coordonné par

STICHTING AMSTERDAM UMC
Netherlands

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (15)

Protocol for establishing the gut-epithelial model in STACKS [M6]. A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided. [R] [PU]

Protocol for establishing the gutepithelial model in STACKS M6 A standard protocol for the transfer of gut epithelial monolayer model from Transwell to STACKS will be provided R PU

Report on diffusion properties through the Biosilk matrix [M12]. Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported. [R] [PU]

Report on diffusion properties through the Biosilk matrix M12 Diffusion properties of fluorochromes in the Biosilk network with and without cells will be reported R PU

Definition of standard values for specific immune response parameters in the gut epithelial-immune cell co-culture model [M24]. A report on the measured standard values for immune markers will be available. [R] [PU]
Manuscript on the effect of fibroblasts on gut epithelial differentiation [M24]. The role of fibroblasts within the Biosilk network to effect gut differentiation in vitro will be elucidated. [R] [PU]
Manuscript describing viral infection of the gut mucosa model [M42] [R] [PU]
Protocol for cytotoxicity assay in gut mucosa model [M30] [Report on the best method for cytotoxicity assessment] [R] [PU]
Manuscript on antiviral testing in complex gut/brain models [M48] [R] [PU]
Protocol for iPSC-derived BBB model [M24] [A standard protocol for establishing a BBB model will be provided]. [R] [PU]
Data Management Plan [M6, M30, M48] Regular updates of DMP is uploaded to the website. [R] [PU]
Manuscript on the method to co-culture gut-epithelial model with immune cells [M24]. [R] [PU]
Report on the properties of the Biosilk network [M24]. The complete properties of the Biosilk network such as tensile and cell motility will be characterised. [R] [PU]
Characterisation panel for iPSC-derived BBB model. [M24] [Report on standard range of parameters for BBB model] [R] [PU]
Manuscript describing viral infection of the BBB-brain model [M42] [R] [PU]
Report on the properties of the Biosilk nanomembrane [M24]. The complete properties of the Biosilk nanomembrane such as porosity and mass transport will be characterised. [R] [PU]
"Protocol for patterning ""crypt-villi"" features on Biosilk [M9]. A standard protocol for embossing or casting ""crypt-villi"" structures will be delivered. [R] [PU]"

Protocol for patterning cryptvilli features on Biosilk M9 A standard protocol for embossing or casting cryptvilli structures will be delivered R PU

Demonstrators, pilots, prototypes (2)

Demonstrator of BBB-brain model [M36] A co-culture of BBB-brain is established [DEM] [PU]
Demonstrator of infection along the gut-brain axis [M48]. As a proof-of-principle application, the viral infection will be shown in the assembled gut-brain axis OoC. [DEM] [PU]

Open Research Data Pilot (1)

Open Research Data Pilot

Other (1)

GUTVIBRATIONS Website [M1]. Public release of the website [OTHER] [PU]

Publications

Peer reviewed articles (16)

What is a neurotropic virus: Discrepancies in terminology between clinical and basic science

Auteurs: Pamela E. Capendale, Katja C. Wolthers, Dasja Pajkrt
Publié dans: Med, Numéro 4, 2024, Page(s) 660-663, ISSN 2666-6340
Éditeur: Elsevier BV
DOI: 10.1016/j.medj.2023.07.001

A quantitative meta-analysis: Is organ on a chip cell cultures better than static cell cultures?

Auteurs: Martin Dufva
Publié dans: Scientific Reports, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.21203/rs.3.rs-2208798/v1

Parechovirus infection in human brain organoids: host innate inflammatory response and not neuro-infectivity correlates to neurologic disease

Auteurs: Pamela E. Capendale, Inés García-Rodríguez, Anoop T. Ambikan, Lance A. Mulder, Josse A. Depla, Eline Freeze, Gerrit Koen, Carlemi Calitz, Vikas Sood, Renata Vieira de Sá, Ujjwal Neogi, Dasja Pajkrt, Adithya Sridhar, Katja C. Wolthers
Publié dans: Nature Communications, Numéro 15, 2024, ISSN 2041-1723
Éditeur: Nature Publishing Group
DOI: 10.1038/s41467-024-46634-9

Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures

Auteurs: Giulia Moreni, Hetty van Eijk, Gerrit Koen, Nina Johannesson, Carlemi Calitz, Kimberley Benschop, Jeroen Cremer, Dasja Pajkrt, Adithya Sridhar, Katja Wolthers
Publié dans: Viruses, Numéro 15, 2025, Page(s) 1823, ISSN 1999-4915
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v15091823

Tissue Like Density Sheets of Hepatoblastoma Cultures Obtained with Thin Suspended Hydrogels Mimicking In Vivo Inter‐Capillary Distances

Auteurs: Alexander Jönsson, Hakan Gürbüz, Louise Wildfang, Pernille Rose Jensen, Martin Dufva
Publié dans: Advanced Materials Technologies, 2024, ISSN 2365-709X
Éditeur: Advanced Materials
DOI: 10.1002/admt.202401204

Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022

Auteurs: Nikita Narayan Naik, Bharadwaja Vadloori, Suresh Poosala, Pratima Srivastava, Sandra Coecke, Adrian Smith, Aysha Akhtar, Clive Roper, Sridhar Radhakrishnan, Balaji Bhyravbhatla, Madhujit Damle, Venkat Koushik Pulla, Johannes Hackethal, Reyk Horland, Albert P. Li, Falguni Pati, Manu Smriti Singh, Paola Occhetta, Rohit Bisht, Prajakta Dandekar, Krishna Bhagavatula, Dasja Pajkrt, Michael Johnson, Til
Publié dans: Alternatives to Laboratory Animals, Numéro 51, 2025, Page(s) 263-288, ISSN 0261-1929
Éditeur: Fund for the Replacement of Animals in Medical Experiments
DOI: 10.1177/02611929231180428

Assessment of the broad-spectrum host targeting antiviral efficacy of halofuginone hydrobromide in human airway, intestinal and brain organotypic models.

Auteurs: Inés García-Rodríguez, Giulia Moreni, Pamela E. Capendale, Lance Mulder, Ikrame Aknouch, Renata Vieira de Sá, Nina Johannesson, Eline Freeze, Hetty van Eijk, Gerrit Koen, Katja C. Wolthers, Dasja Pajkrt, Adithya Sridhar, Carlemi Calitz
Publié dans: Antiviral Research, Numéro 222, 2024, Page(s) 105798, ISSN 0166-3542
Éditeur: Elsevier BV
DOI: 10.1016/j.antiviral.2024.105798

Enhanced biocompatibility of 3D printed resin parts <i>via</i> wet autoclave postprocessing: implications for stem cell organ-on-a-chip culture

Auteurs: Alexander Jönsson, Antonia Iatrou, Louise Wildfang, Dana J. Neumann, Hakan Gürbüz, Carina A. A. Schoenmaker, Marlene Danner Dalgaard, Pernille Rose Jensen, Martin Dufva
Publié dans: Materials Advances, 2025, ISSN 2633-5409
Éditeur: Materials Advances
DOI: 10.1039/d4ma01191k

A beginner’s guide on the use of brain organoids for neuroscientists: a systematic review

Auteurs: Lance A. Mulder, Josse A. Depla, Adithya Sridhar, Katja Wolthers, Dasja Pajkrt, Renata Vieira de Sá
Publié dans: Stem Cell Research &amp; Therapy, Numéro 14, 2023, ISSN 1757-6512
Éditeur: BioMed Central
DOI: 10.1186/s13287-023-03302-x

Editorial: Human organoid technology for virus research

Auteurs: Dasja Pajkrt, Veronica Krenn, Joana Rocha-Pereira
Publié dans: Frontiers in Cellular and Infection Microbiology, Numéro 13, 2023, ISSN 2235-2988
Éditeur: Frontiers Media S. A.
DOI: 10.3389/fcimb.2023.1155252

Understanding neurotropic enteric viruses: routes of infection and mechanisms of attenuation

Auteurs: Valeria Lulla, Adithya Sridhar
Publié dans: Cellular and Molecular Life Sciences, Numéro 81, 2024, ISSN 1420-682X
Éditeur: Birkhauser Verlag
DOI: 10.1007/s00018-024-05450-6

A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures

Auteurs: Martin Dufva
Publié dans: Scientific Reports, Numéro 13, 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-023-35043-5

Evaluation of 3D Human Intestinal Organoids as a Platform for EV-A71 Antiviral Drug Discovery

Auteurs: Fatma Masmoudi, Nanci Santos-Ferreira, Dasja Pajkrt, Katja C. Wolthers, Jeroen DeGroot, Maria L. H. Vlaming, Joana Rocha-Pereira, Ludovico Buti
Publié dans: Cells, Numéro 12, 2023, Page(s) 1138, ISSN 2073-4409
Éditeur: MDPI AG
DOI: 10.3390/cells12081138

Current and Future Antiviral Strategies to Tackle Gastrointestinal Viral Infections

Auteurs: Nanci Santos-Ferreira, Jana Van Dycke, Johan Neyts, Joana Rocha-Pereira
Publié dans: Microorganisms, Numéro 9, 2024, Page(s) 1599, ISSN 2076-2607
Éditeur: Microorganisms
DOI: 10.3390/microorganisms9081599

Human milk inhibits some enveloped virus infections, including SARS-CoV-2, in an intestinal model

Auteurs: Ikrame Aknouch, Adithya Sridhar, Eline Freeze, Francesca Paola Giugliano, Britt J van Keulen, Michelle Romijn, Carlemi Calitz, Inés García-Rodríguez, Lance Mulder, Manon E Wildenberg, Vanesa Muncan, Marit J van Gils, Johannes B van Goudoever, Koert J Stittelaar, Katja C Wolthers, Dasja Pajkrt
Publié dans: Life Science Alliance, Numéro 5, 2024, Page(s) e202201432, ISSN 2575-1077
Éditeur: Frontiers
DOI: 10.26508/lsa.202201432

Amino acid variation at VP1-145 of enterovirus A71 determines the viral infectivity and receptor usage in a primary human intestinal model

Auteurs: Ikrame Aknouch, Inés García-Rodríguez, Francesca Paola Giugliano, Carlemi Calitz, Gerrit Koen, Hetty van Eijk, Nina Johannessson, Sjoerd Rebers, Lieke Brouwer, Vanesa Muncan, Koert J. Stittelaar, Dasja Pajkrt, Katja C. Wolthers, Adithya Sridhar
Publié dans: Frontiers in Microbiology, Numéro 14, 2023, ISSN 1664-302X
Éditeur: Frontiers Media
DOI: 10.3389/fmicb.2023.1045587